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NOV issue of Journal of Microscopy online now!

In this issue: A Review of Resolution & optical sectioning in the confocal microscope. Take a closer look...

invited Review

Resolution and optical sectioning in the confocal microscope
T. WILSON

In this short review, we present a self-contained discussion of the image formation properties of the fluorescent confocal microscope. The optical sectioning or depth discrimination property is discussed in detail and new analytic formulae are presented, which relate the optical sectioning strength to the wavelength, numerical aperture and pinhole aperture size in a particularly simple fashion.

Original Articles

Journal Sept 2011

The feasibility of Cryo In-SEM Raman microspectroscopy
J. HAZEKAMP, M. G. REED, C.V. HOWARD, A. A. VAN APELDOORN and C. OTTO

Experiments and quantitative analysis of frequency division multiplexing confocal fluorescence microscopy with UV excitation J. ZHENG, Y. JIANG, Y. ZHANG, P. TANG, A. HUANG and S. ZHUANG

Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversity
D. DÉBARRE, T. VIEILLE and E. BEAUREPAIRE

Distinguishing the parallel and vertical orientations and optic axis characteristics determination of azobenzene mesogen by conoscopic polarized microscopy
J. LIU, M. WANG, M. DONG, L. GAO and J. TIAN

Imaging of Bernal stacked and misoriented graphene and boron nitride: experiment and simulation
R. ZAN, U. BANGERT, Q. RAMASSE and K.S. NOVOSELOV

Figure of merit for environmental SEM and its implications
G. D. DANILATOS

Atom probe trajectory mapping using experimental tip shape measurements
D. HALEY, T. PETERSEN, S.P. RINGER and G.D.W. SMITH

A novel method for acquiring large-scale automated scanning electron microscope data
A.R. SHIVELEY, P.A. SHADE, A.L. PILCHAK, J.S. TILEY and R. KERNS

Quantitative detection of gold nanoparticles on individual, unstained cancer cells by scanning electron microscopy
L. HARTSUIKER, P. VAN ES, W. PETERSEN, T.G. VAN LEEUWEN, L.W.M.M. TERSTAPPEN and C. OTTO

Automated and semi-automated cell tracking: addressing portability challenges
A. KAN, R. CHAKRAVORTY, J. BAILEY, C. LECKIE, J. MARKHAM and M.R. DOWLING

On the sampling of three-dimensional polycrystalline microstructures for distribution determination
J. LUAN, G. LIU, H. WANG and A. ULLAH

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