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Location:
NIMR, UK
Date:
June 2007
Contact:
Event Organiser
Tel:
01865 248768
Fax:
01865 791237
Email:
events@rms.org.uk
Website:
none
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WORKSHOP OUTLINE
Modern light microscopy is highly quantitative and involves collection, storage and analysis of digital images. The recent revolution in domestic digital imaging means that many people are now familiar with digital imaging and some will have experience of basic image processing and image manipulation.
The aim of this one-day RMS workshop meeting is to cover the underlying principles of digital image processing and allow the advanced user of light microscopy to make the very best use of their imaging data. The types of image vary from those of high spatial resolution obtained using confocal microscopy to lower resolution video and multidimensional data obtained using advanced optical techniques (like FRET, FRAP, multiphoton).
This workshop course consists of two lectures that introduce the basic concepts of digital image processing and image analysis. The main focus of the day is for delegates to participate in a hands-on, computer-based workshop in which digital image analysis will be learnt by example. Workshop materials will be provided in advance of the meeting in the form of a CDROM and worksheets.
The small group, workshop format, will enable people to progress at their own pace and work through the basics of signal processing and data analysis using Excel spreadsheets and progressing to image processing and image analysis using ImageJ. The course will be supported by expert demonstrators.
The aims of the course are firstly to advance the understanding and use of digital image processing and image analysis tools and secondly to initiate a network of interactions between young UK researchers that will be mediated and supported by the Light Microscopy Section of the RMS.
Topics that will be covered:
Nature of digital images; storing and handling raw images - image formats; data compression - problems and advantages; loading, saving and printing images; scales (time and space); frames/second, nanometres/pixels; the nature of data obtained from digital cameras versus video cameras; concepts of binning, interlacing, frame transfer, field transfer frame; grabbers - analogue versus digital.
Event Programme
Outline Programme (subject to change)
09:45 Coffee and registration
10:00 Welcome from Justin Molloy
10:05 David Holburn, University of Cambridge
An introduction to digital image processing and image analysis
10:45 Practical workshop on data smoothing and signal processing of 1 and 2 dimensional datasets
12:30 - 13:30 Lunch and RMS Light Microscopy Section AGM
13:30 Paul Sheppard, Imaging Associates
The principles and methods of image processing and image analysis as applied to optical microscopy
Lecture on video microscopy and multidimensional images
14:10 Practical workshop on dealing with 2D and 3D datasets - more on smoothing, filtering and object recognition and rejection
15:30 Tea
15:50 Workshop continues on specialist techniques - image arithmetic, Fourier transform, kernals and convolution, B/W binary data, image statistics
5:30 FINISH
