The course will enable students to fully appreciate and utilise the confocal microscope with confidence and help participants realise the potential of confocal imaging, beyond taking pretty pictures. Participants will develop their confocal microscopy background as well as try FRAP, FRET and spectral unmixing themselves. The confocal course will utilise many different sample types and fluorescent probes (DNA stains, classic antibody labels and fluorescent proteins) and will be chosen to best demonstrate particular problems and techniques. The focus will be on the techniques that they enable and the problems they generate, which will be applicable to any sample types. The 2-Days will consist of short tutorials followed by hands-on practice. Day 1 will take the participants through the basic principles of confocal microscopy and then train through hands-on practice how to configure and image multicolour, multidimensional samples using a confocal microscope. Day 2 will build on the experiences of day 1 and enable participants to try FRAP and spectral profiling. This course has been awarded 16 CPD points by the IBMS This course is preceded by a 3 day Light Microscopy Course. Click here for further information on the Light Microscopy Summer School.
|