 Provisional Programme
Tuesday, 17th April:
15:00- 17:00 Arrival and registration
Opening Session
17:00-17:45 Tony Wilson University of Oxford
Making light work in microscopy
17.45- 18.30 Bas Ploem, Leiden University, The Netherlands
Early developments in fluorescence microscopy
19.00 - Late Welcome drinks reception and buffet
Wednesday, 18th April:
Session 1 New Application/Technologies
08:50 - 09:20 Ernst Stelzer, Cell Biology & Biophysics Unit, EMBL, Germany
Light-sheet based fluorescence microscopy (SPIM) supports a modern approach to a three-dimensional cell biology
09:20 - 09:50 Peter Kner, Department of Biochemistry and Biophysics, UCSF, USA
Recent Advances in Microscopy at UCSF
09:50 - 10:10 Kate Poole, JPK Instruments AG, Berlin, Germany
Fully integrated atomic force and optical microscopy- combining nanoscale measurements with far field optics
10:10 - 10:30 Jim Swoger, Centre for Genomic Regulation, Barcelona, Spain
Optical Projection Tomography (OPT): Multidimensional Imaging of Intact Embryos & Organs
10:30 - 11:00 Coffee/tea break
Session 2 Live cell imaging
11:00 - 11:30 Erik Manders, Centre for Advanced Microscopy, University of Amsterdam, The Netherlands
Controlled light exposure microscopy (CLEM) for prolonged live-cell imaging
11:30 – 11:50 Ian Dobbie, Genome Stability Laboratory, Galway, Ireland
Fluorescence correlation spectroscopy and fluorescent proteins: measuring diffusion rates, concentrations and protein-protein interactions
11:50 - 12:10 Kota Miura, Centre for Molecular and Cellular Imaging, EMBL, Germany
Fluorescence Images as the Initial Condition for Stochastic Simulations - studies on the effect of intracellular geometry on the biochemical parameters of ER exit sites
12:10 - 12:30 Adriaan Houtsmuller, Department of Pathology, Josephine Nefkens Institute, Rotterdam, The Netherlands
Simultaneous FRAP and FRET reveal compartmentalisation of androgen receptor protein-protein interactions in living cells
12:30 – 12:50 Spencer Shorte, Institut Pasteur, Paris, France
A system and methodology for high-content visual screening of individual intact living cells in suspension
13:00-14:00 Buffet lunch
14:00-15:30 Workshops
15:30-16:00 Coffee/tea break
16:00-17:30 Workshops
Session 3 High content imaging
17:35 - 18:05 Mike White, Department of Biological Sciences, University of Liverpool, UK
Use of live cell microscopy for analysis of the NF-kB system
18:05 - 18:25 Heimo Wolinski, Institute of Molecular Biosciences, University Graz, Austria
High-content cell-based profiling of peroxisome characteristics in yeast mutants
18:25 - 18:45
19:00-20:30 Dinner
21:00-23:00 Poster session
Thursday, 19th April:
Session 4 Flourescent proteins
08:50 - 09:20 Alberto Diaspro, Department of Physics, University of Genoa, Italy
Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging
09:20 - 09:50 John Runions, School of Life Sciences, Oxford Brookes University, UK
Photoactivation of GFP for quantification of intramembrane protein dynamics
09:50 - 10:10 Arne Seitz, Advanced Light Microscopy Facility, EMBL, Germany
Quantitative measurement of the YFP to CFP photoconversion
10:10 - 10:30 Trygve Bergeland, Department of Molecular Biosciences, University of Oslo, Norway
Cell cycle regulated binding kinetics for early endosomal coat proteins
10:30-11:00 Coffee/tea break
Session 5 Sub cellular signalling/Molecular imaging
11:00 - 11:30 Paul French, Department of Physics, Imperial College London, UK
Multidimensional Fluorescence Imaging
11:30 - 12:00 Erik Manders, Centre for Advanced Microscopy, University of Amsterdam, The Netherlands
Phototoxicity in live cell imaging
12:00 - 12:30 Philipp Kukura, ETH Zurich, Laboratory of Physical Chemistry, Switzerland
Detecting and visualizing the motion of single nano-objects
12:30 – 13:00 Carsten Schultz, EMBL
Imaging novel aspects of growth factor signaling
13:00-14:00 Buffet lunch
14:00-15:30 Workshops
15:30-16:00 Coffee/tea break
16:00-17:30 Workshops
Session 6 Correlative imaging
17:35 - 18:05 Paul Monaghan, Bioimaging, IAH, Pirbright, UK
18:05 - 18:25 Mark Coles, Immunology and Infection Unit, Department of Biology and HYMS, University of York, UK
Imaging the early phase immune response to Pneumoniae infection
18:25 - 18:45 Paul Verkade, School of Medical Sciences, University of Bristol, UK
Moving EM: Correlative microscopy with high time resolution and optimal structural preservation
19:30-late Conference dinner (National Railway Museum)
Friday, 20th April:
Session 7 Single molecule studies/High resolution microscopy
08:50 - 09:20 Justin Molloy, MRC National Institute for Medical Research, London, UK
Single molecule mechanical and optical studies: in vitro and inside live mammalian cells
09:20 - 09:50 Rainer Heintzmann, Randall Division, King’s College London, UK
Fluorescence microscopy with the help of computational spectacles
09:50 - 10:10 Mark Leake, Department of Physics, University of Oxford, UK
Monitoring number and turnover of stator units in functional rotary flagellar motors of living bacterial cells in real-time using single-molecule fluorescence microscopy
10:10 - 10:30 Yury Belyaev, Advanced Light Microscopy Facility, EMBL, Germany
A variable incidence angle total internal reflection system with uniform illumination intensity
10:30-11:00 Coffee/tea break
11:00-13:00 Workshops
13:00-14:00 Buffet lunch
Session 8 Analysis/Stereology
14:00 - 14:30 Sinan Yuruker, Department of Histology, Hacettepe University, Ankara, Turkey
Stereology
14:30 – 14:50 Jeremy Adler, Department of Cell Biology, Stockholm University, Sweden
Replicate Based Noise Corrected Correlation (RBNCC) for Accurate Measurements of Colocalization
14:50 – 15:10 Timo Zimmermann, Advanced Light Microscopy Unit, Centre de Regulació Genňmica, Barcelona, Spain
Multiparameter analysis of sensitized emission FRET data
15:10 – 15:30 Mathieu Marchand, Institut Pasteur, Paris & Rockefeller University, New York
The Pasteur Platform Management System (PPMS): A database orientated web-interfaced software for technical research facility management
Concluding remarks
Please visit the ELMI website for further information.
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