This intensive course included teaching sample preparation and cryo-microscopy techniques for both scanning and transmission electron microscopy. Participants from both academia and industry looking to learn new skills or update their knowledge attended.
The Bioimaging Unit at Rothamsted Research is a state-of-the-art facility for cryo electron microscopy in partnership with Gatan UK, Leica UK and Jeol UK. It is equipped with both scanning and transmission electron microscopes.
Speakers:
Plenary speaker – Kent MacDonald (University of California Berkeley)
Cryofixation – Roger Wepf (ETH Zurich, EMEZ)
Cryo sectioning – Helmut Gnaegi (Diatome)
CryoSEM – Kim Findlay (John Innes Centre)
CryoTEM – Dave Bhella (University of Glasgow)
Freeze substitution and sample processing - Paul Verkade (University of Bristol)
Optimising camera and software variables - Neil Wilkinson (Gatan)
Common artifacts - Mailyn Carey (Gatan)
Further Information
Topic: Techniques covered will include the Tokuyasu method, rapid freezing of samples including high pressure freezing, freeze substitution, cryo-sectioning, thin film TEM and cryo-transfer and freeze fracture cryo-SEM. Attendees will have the opportunity to use state of the art equipment and instrumentation from a range of manufacturers. Format: Tutorials, demonstrations and hands-on practical sessions will be led by technical experts from Gatan, Leica, Diatome and Jeol. Keynote speakers will supplement the techniques taught.
Location/Airport: London-Luton airport is 10 mins away by frequent train and offers low cost flights from most European countries.
Transportation: Rothamsted Research has excellent rail and road links.Central London is 25 minutes by frequent train and the M1 motorway (exit J9) is within 5 miles.
Accommodation: Student style accommodation with shared bathrooms is available on-site at Rothamsted Manor. Please note that the rooms are not en-suite and very basic.
Click here to download a PDF of the 2010 Programme.
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