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Flow Cytometric Analysis of Cell Proliferation and Cell Death
Location:
London Research Institute, Cancer Research UK
Date:
01 December 2008
Contact:
Gemma Bantock
Tel:
01865 254768
Fax:
01865 791237
Email:
gemma@rms.org.uk
Website:
Registration for this meeting is now closed, it is fully subscribed.
Event Description
Scientific Organiser: Derek Davies
This one day course will introduce the basic concepts of using flow cytometry to analyse DNA content in mammalian cells. Starting with the basics of a DNA histogram, there will be lectures on cell cycle kinetics using the bromodeoxyuridine technique, cell proliferation using Hoechst or CFSE, analysis of cell death by apoptosis and combining surface or intracellular staining with DNA content measurement. This course assumes some knowledge of flow cytometry but is of interest to those who wish to expand their expertise or who are embarking on cytometric studies of DNA.
The course will be delivered by Michael Ormerod (Scientific Consultant, Reigate, Surrey) and Derek Davies (Head of the Flow Cytometry Facility at the London Research Institute, Cancer Research UK).
PLEASE NOTE THAT THIS COURSE IS LIMITED TO 35 PARTICIPANTS SO EARLY BOOKING IS VITAL TO SECURE A PLACE.
Event Programme
Cytometric Analysis of Cell Proliferation and Death 2008
Monday 1st December
09.45 - 10.15 Registration and Coffee
10.15 Welcome
(Derek Davies)
10.30 Overview
(Michael G Ormerod)
11.00 DNA Measurement and Cell Cycle Analysis
(Michael G Ormerod)
11.45 Measurement of DNA and Intracellular Antigens
(Derek Davies)
12.20 Lunch and Trade Exhibition
13.30 Assays for Cell Death and Apoptosis
(Derek Davies)
14.10 Measuring Cell Proliferation using BrdUrd/anti-BrdUrd
(Michael G Ormerod)
14.50 Other Methods for Measuring Cell Proliferation
1. Using Hoechst/BrdUrd/PI
2. Dye Dilution
(Michael G Ormerod)
15.30 Coffee
15.50 Multiplexing assays: combining Edu and Fluorescent protein detection
(Lola Martinez)
16.10 Flow cytometric assessment of apoptosis by simultaneous detection of phosphorylated H2AX and loss of cytochrome c
(Laura Bazley)
16.30 Closing Remarks
(Derek Davies)
Timings are subject to small changes
