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The Practicalities of Flow Cytometry
Location:
Newcastle Upon Tyne
Date:
11 February 2009
Contact:
Ian Dimmick or Rebecca Stewart
Tel:
Fax:
Email:
ian.dimmick@ncl.ac.uk
Website:
none
Event Description
Venue: The Chandelier Suite, The Assembly Rooms, Fenkle Street, Newcastle-Upon-Tyne, NE1 5XU.
This meeting is designed in the first part to teach a generic understanding of the basics of flow cytometry, focusing on instrument daily maintenance troubleshooting and quality control.
The issues of experimental set up and considerations of differing instrument architecture and their impact on sensitivity, fluorochrome choice and opportunities with relatively new reagents such as nano particles and new fluorochromes will be explored.
One aspect of basic flow cytometry that deserves special consideration which will be dealt with in a little more depth is colour compensation, as well as data analysis and the basics of cell sorting.
Within a techniques and applications session we will deal with more specific subject areas including Telomere length, Apoptosis and a clinical application of cell sorting .
This meeting is well sponsored therefore we can keep the registration fee to a nominal 25 .
In addition for those delegates who wish to there is a complimentary Flo Jo seminar the day prior to this meeting at 4.30pm within the Institute of Human Genetics.This is timed so that anyone travelling and arriving in Newcastle the day before the meeting is most welcome to attend.
To apply for a registration form please email Ian.dimmick@ncl.ac.uk or R.Stewart@ncl.ac.uk
Event Programme
0900-0920 Refreshments
0920-0930 Welcome
R Stewart (Newcastle University)
Introduction to instrumentation
0930-1000 Becton Dickinson instrumentation and quality control
(Instrumentation layout, fluidics, filters and photodetectors)
M Dyson (Becton Dickinson)
1000-1030 Beckman Coulter instrumentation and quality control (Instrumentation layout, fluidics, filters and photodetectors)
M Cheetham (Beckman Coulter)
1030-1100 Coffee and trade exhibition
Experimental setup and multicolour flow cytometry
1100-1130 Fluorescence, fluorochromes and isotype controls
(Fluorescence explained, fluorochrome choice, antibody titration, isotype controls)
I Dimmick (Newcastle University)
1130-1200 Multicolour flow cytometry and compensation
(Spectral overlap explained, the dos and donts of compensation, problems and solutions)
R Stewart (Newcastle University)
Fluorescence-activated cell sorting
1200-1245 Cell sorting: An overview
J Lawry (Becton Dickinson)
1245-1400 Lunch and trade exhibition
Data analysis
1400-1430 Flow cytometric data analysis
C Vallan (Flowjo, Treestar Inc.)
1430-1500 FCS File Structure & Analysis Using VenturiOne
P Nobes (Applied Cytometry)
1500-1530 Coffee and trade exhibition
Techniques and applications
1530-1600 Flow Cytometric analysis fo the cell cycle and cell proliferation
Annemieke Walker(Invitrogen)
1600-1630 Measurement of telomere length in white blood cells by flow cytometry
I Spyridopoulos (Cardiology, Newcastle University)
1630-1700 Cytometric assessment of apoptotic cell death
D Davies (CRUK)
1700-1730 Flow cytometry and fluorescence-activated cell sorting of the human dermis
M Haniffa (Academic Haematology, Newcastle University)
1730-1745 Closing remarks
R Stewart (Newcastle University)
