Crick EM Symposium: Focus on Correlative Imaging Across Scales
Continuing on from our 2017 Crick EM Symposium that celebrated the opening of the EM facilities, we will hold a two-day symposium exploring the impact of correlative imaging on biomedical research across scales. The symposium will include talks from research and technology leaders in imaging, from the macromolecular through to the whole organism scales, with a particular emphasis on how these new techniques can be applied to biomedical research. We will also hear about cutting edge developments in correlative imaging and big microscopy data. There will be a large interactive exhibition from our commercial collaborators.
Please note, this is a provisional programme and may still be subject to change
- 09:30 Arrival and Registration
- 09:50 Welcome: Lucy Collinson and Raffaella Carzaniga
Session 1: Molecules in Cells
Chair: Peter Rosenthal (Francis Crick Institute, UK)
- 10:00 "Adventures in Automation for CryoEM" - Bridget Carragher (New York Structural Biology Center, USA)
- 10:30 "Molecular strategies for regulating microtubule dynamics" - Carolyn Moores (Institute of Structual and Molecular Biology, Birkbeck College, UK)
- 11:00 "Structural biology across spatial scales: atomic structures to cellular function" - Tanmay Bharat (Oxford University, UK)
- 11:30 "New opportunities for cryo tomography by cryo-STEM tomography" - Michael Elbaum (Weitzmann, Israel)
- 12:00 Lunch and Exhibition
Session 2: Molecules in Cells
Chair: Raffaella Carzaniga (Francis Crick Institute, UK)
- 13:30 "Beauty and benefit of Cryo-LM & Cryo-EM: our achievements with method development and the Type 7 Secretion System in M. tuberculosis" - Peter Peters (Maastricht University, The Netherlands)
- 14:00 "Towards a mechanistic understanding of â€¨ciliary assembly with CLEM and cryo-EM" - Gaia Pigino (MPI Dresden, Germany)
- 14:30 "Imaging complement activation using phase-plate cryoET; from initiation to pore formation" - Thomas Sharpe (Leiden University Medical Centre, The Netherlands)
- 15:00 Tea/Coffee and Exhibition
Session 3: Cells
Chair: Paul Verkade (Bristol University, UK)
- 16:00 "Deciphering the Role of Infection Associated Macropinosomes by Large Volume CLEM" - Jost Enninga (Institut Pasteur, France)
- 16:30 Title TBC - Lorna Hodgson (Bristol University, UK)
- 17:00 Title TBC - Jeremy Carlton (Francis Crick Institute, London, UK)
- 17:30 "Trafficking pathways to the lysosome" - Clare Futter (UCL Institute of Ophthalmology, UK)
- 18:00 Drinks Reception
- 09:30 Arrival and Registration
Session 4: Tissues
Chair: Yannick Schwab (EMBL, Heidelberg, Germany)
- 10:00 "From gene expression to specialised morphology: Exploring the evolution of cell types using correlative volume EM" - Rachel Templin (EMBL, Heidelberg, Germany)
- 10:30 Title TBC - Eva Frickel (Francis Crick Institute, London, UK)
- 11:00 "Adding enhanced localization information to 3D EM volumes" - Christian Stigloher (Imaging Core Facility, Biocenter, University of Würzburg)
- 11:30 "3D EM Reveals the Connectivity of Modulatory Neurons in the Adult Brain" - Graham Knott (EPFL, Lausanne, Switzerland)
- 12:00 Lunch and Exhibition
Session 5: New Frontiers - Correlative Imaging
Chair: Thea Stewart (King's College London, UK)
- 13:30 "High-throughput 3D CLEM with integrated and faster electron microscopes" - Jacob Hoogenboom (TU Delft, The Netherlands)
- 14:00 "Correlative X-ray phase contrast, X-ray fluorescence and EM to explore tissues and cells in 3D at the nanoscale" - Alexandra Pacaneau (ESRF, France)
- 14:30 Title TBC - Martin Jones (Francis Crick Institute, UK)
- 15:00 Tea/Coffee and Exhibition
Session 6: New Frontiers - Modelling and Image Analysis
Chair: Martin Jones (Francis Crick Institute, London, UK)
- 16:00 Title TBC - Stephan Preibisch (MDC Berlin, Germany)
- 16:30 "Automated cell segmentation at scale" - Anna Kreshuk (EMBL, Heidelberg, Germany)
- 17:00 "Untangling abnormal retinal vascular tufts" - Katie Bentley (King's College London / Francis Crick Institute, London)
- Session 7: The Future of Correlative Imaging
- 17:30 "Imaging Across Scales & Institutes" - Andreas Walter (BioImaging Austria - CMI)
- 18:00 Drinks Reception
Dr Lucy Collinson
EM Section Chair
The Francis Crick Institute
Lucy is Head of Electron Microscopy at The Francis Crick Institute in London. Her degree and PhD were in Microbiology, followed by a post-doctoral position in Cell Biology using light and electron microscopy to investigate membrane trafficking pathways at University College London. Following that she ran biological EM facilities, first at UCL and then at the Cancer Research UK London Research Institute, which became part of the new Francis Crick Institute in 2015. Her microscopy interests cover 3D EM, Correlative Light and EM, X-ray microscopy, image analysis, and microscope design and prototyping.
Francis Crick Institute, UK
Prof Paul Verkade
University of Bristol
Paul Verkade has been involved in electron microscopy for over 20 years now and during that time has developed a particular interest in technology development, mainly in the field of Correlative Light Electron Microscopy. He loves organising training events and as such has been involved in many courses. For the RMS he is involved in the Rothamsted Research Cryo Course and was a member of the organising committee for emc2012.
Molecules in Cells
- Bridget Carragher (NYCSB, USA)
- Carolyn Moores (Birkbeck, UK)
- Tanmay Bharat (Oxford University, UK)
- Michael Elbaum (Weitzmann, Israel)
- Peter Peters (Maastricht University, The Netherlands)
- Gaia Pigino (MPI Dresden, Germany)
- Thom Sharpe (LUMC, The Netherlands)
- Jost Enninga (Institut Pasteur, France)
- Lorna Hodgson (Bristol University, UK)
- Jeremy Carlton (Francis Crick Institute, UK)
- Clare Futter (Institute of Ophthalmology, UK)
- Eva Frickel (Francis Crick Institute, UK)
- Christian Stigloher (University of Wurzburg, Germany)
- Graham Knott (EPFL, Switzerland)
- Rachel Templin (EMBL, Germany)
New Frontiers - Correlative Imaging
- Jacob Hoogenboom (TU Delft, The Netherlands)
- Alexandra Pacaneau (ESRF, France)
- Martin Jones (Francis Crick Institute, UK)
New Frontiers - Modelling and Image Analysis
- Katie Bentley (Francis Crick Institute, UK)
- Anna Kreshuk (EMBL, Germany)
- Stephan Preibisch (MDC Berlin, Germany)
The Future of Correlative Imaging
- Andreas Walther (BioImaging Austria)
Please note, there is an early-bird offer available until Sunday 2 June. After this date, the registration fee will raise by £20.
The symposium will be immediately followed by the 2019 UK Correlative Light and Electron Microscopy Course, from Wednesday 17th to Friday 19th July. The hands-on CLEM course will cover three correlative workflows - 3D CLEM, Integrated Light and EM, and cryo CLEM - using a range of techniques and equipment including Near Infrared Branding, micro CT, Serial Block Face SEM, Focused Ion Beam SEM, high pressure freezing, in-resin fluorescence, ultraLM, integrated light and SEM (SECOM), plunge freezing and cryo-fluorescence. There will also be a CLEM image analysis tutorial session. There are limited places available on the CLEM course.
Please contact Raffaella Carzaniga to express interest.
Location: Francis Crick Institute, London, NW1 1ST
If you are interested in sponsoring this meeting, please contact Kate Jermey.
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Labtech International Ltd
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