Crick EM Symposium: Focus on Correlative Imaging Across Scales
Continuing on from our 2017 Crick EM Symposium that celebrated the opening of the EM facilities, we will hold a two-day symposium exploring the impact of correlative imaging on biomedical research across scales. The symposium will include talks from research and technology leaders in imaging, from the macromolecular through to the whole organism scales, with a particular emphasis on how these new techniques can be applied to biomedical research. We will also hear about cutting edge developments in correlative imaging and big microscopy data. There will be a large interactive exhibition from our commercial collaborators.
Please note, this is a provisional programme and may still be subject to change
- 09:30 Arrival and Registration
- 09:50 Welcome: Lucy Collinson and Raffaella Carzaniga
Session 1: Molecules in Cells
Chair: Peter Rosenthal (Francis Crick Institute, UK)
- 10:00 "Adventures in Automation for CryoEM" - Bridget Carragher (New York Structural Biology Center, USA)
- 10:30 "Molecular strategies for regulating microtubule dynamics" - Carolyn Moores (Institute of Structual and Molecular Biology, Birkbeck College, UK)
- 11:00 "Structural biology across spatial scales: atomic structures to cellular function" - Tanmay Bharat (Oxford University, UK)
- 11:30 "New opportunities for cryo tomography by cryo-STEM tomography" - Michael Elbaum (Weitzmann, Israel)
- 12:00 Lunch and Exhibition
Session 2: Molecules in Cells
Chair: Raffaella Carzaniga (Francis Crick Institute, UK)
- 13:30 "Beauty and benefit of Cryo-LM & Cryo-EM: our achievements with method development and the Type 7 Secretion System in M. tuberculosis" - Peter Peters (Maastricht University, The Netherlands)
- 14:00 "Towards a mechanistic understanding of â€¨ciliary assembly with CLEM and cryo-EM" - Gaia Pigino (MPI Dresden, Germany)
- 14:30 "Imaging complement activation using phase-plate cryoET; from initiation to pore formation" - Thomas Sharp (Leiden University Medical Centre, The Netherlands)
- 15:00 Tea/Coffee and Exhibition
Session 3: Cells
Chair: Paul Verkade (Bristol University, UK)
- 16:00 "Deciphering the Role of Infection Associated Macropinosomes by Large Volume CLEM" - Jost Enninga (Institut Pasteur, France)
- 16:30 "Correlative imaging of functional synthetic supramolecular structures: Synthetic biology meets cellular biology" - Lorna Hodgson (Bristol University, UK)
- 17:00 "ESCRT-III-dependent nuclear envelope regeneration during mitotic exit" - Jeremy Carlton (Francis Crick Institute, London, UK)
- 17:30 "Trafficking pathways to the lysosome" - Clare Futter (UCL Institute of Ophthalmology, UK)
- 18:00 Drinks Reception
- 09:30 Arrival and Registration
Session 4: Tissues
Chair: Yannick Schwab (EMBL, Heidelberg, Germany)
- 10:00 "From gene expression to specialised morphology: Exploring the evolution of cell types using correlative volume EM" - Rachel Templin (EMBL, Heidelberg, Germany)
- 10:30 "In vivo control of Toxoplasma gondii by zebrafish macrophages" - Eva Frickel (Francis Crick Institute, London, UK)
- 11:00 "Adding enhanced localization information to 3D EM volumes" - Christian Stigloher (Imaging Core Facility, Biocenter, University of Würzburg)
- 11:30 "3D EM Reveals the Connectivity of Modulatory Neurons in the Adult Brain" - Graham Knott (EPFL, Lausanne, Switzerland)
- 12:00 Lunch and Exhibition
Session 5: New Frontiers - Correlative Imaging
Chair: Thea Stewart (King's College London, UK)
- 13:30 "High-throughput 3D CLEM with integrated and faster electron microscopes" - Jacob Hoogenboom (TU Delft, The Netherlands)
- 14:00 "Correlative X-ray phase contrast, X-ray fluorescence and EM to explore tissues and cells in 3D at the nanoscale" - Alexandra Pacureanu (ESRF, France)
- 14:30 "Extracting meaning from Big Data in Electron Microscopy" - Martin Jones (Francis Crick Institute, UK)
- 15:00 Tea/Coffee and Exhibition
Session 6: New Frontiers - Software and Image Analysis
Chair: Martin Jones (Francis Crick Institute, London, UK)
- 16:00 "Correlative Lightsheet and Electron Microscopy in C.elegans" - Stephan Preibisch (MDC Berlin, Germany)
- 16:30 "Automated cell segmentation at scale" - Anna Kreshuk (EMBL, Heidelberg, Germany)
- 17:00 "SBEMimage and VIME: Open-source acquisition and alignment software for serial block-face imaging" - Benjamin Titze (FMI, Basel, Switzerland)
- Session 7: The Future of Correlative Imaging
- 17:30 "Imaging Across Scales & Institutes" - Andreas Walter (BioImaging Austria - CMI)
- 18:00 Drinks Reception
Dr Lucy Collinson
EM Section Chair
The Francis Crick Institute
Lucy is Head of Electron Microscopy at The Francis Crick Institute in London. Her degree and PhD were in Microbiology, followed by a post-doctoral position in Cell Biology using light and electron microscopy to investigate membrane trafficking pathways at University College London. Following that she ran biological EM facilities, first at UCL and then at the Cancer Research UK London Research Institute, which became part of the new Francis Crick Institute in 2015. Her microscopy interests cover 3D EM, Correlative Light and EM, X-ray microscopy, image analysis, and microscope design and prototyping.
Francis Crick Institute, UK
Prof Paul Verkade
University of Bristol
Paul Verkade has been involved in electron microscopy for over 20 years now and during that time has developed a particular interest in technology development, mainly in the field of Correlative Light Electron Microscopy. He loves organising training events and as such has been involved in many courses. For the RMS he is involved in the Rothamsted Research Cryo Course and was a member of the organising committee for emc2012.
Molecules in Cells
- Bridget Carragher (NYCSB, USA)
- Carolyn Moores (Birkbeck, UK)
- Tanmay Bharat (Oxford University, UK)
- Michael Elbaum (Weitzmann, Israel)
- Peter Peters (Maastricht University, The Netherlands)
- Gaia Pigino (MPI Dresden, Germany)
- Thom Sharp (LUMC, The Netherlands)
- Jost Enninga (Institut Pasteur, France)
- Lorna Hodgson (Bristol University, UK)
- Jeremy Carlton (Francis Crick Institute, UK)
- Clare Futter (Institute of Ophthalmology, UK)
- Eva Frickel (Francis Crick Institute, UK)
- Christian Stigloher (University of Wurzburg, Germany)
- Graham Knott (EPFL, Switzerland)
- Rachel Templin (EMBL, Germany)
New Frontiers - Correlative Imaging
- Jacob Hoogenboom (TU Delft, The Netherlands)
- Alexandra Pacureanu (ESRF, France)
- Martin Jones (Francis Crick Institute, UK)
New Frontiers - Modelling and Image Analysis
- Benjamin Titze (FMI, Basel, Switzerland)
- Anna Kreshuk (EMBL, Germany)
- Stephan Preibisch (MDC Berlin, Germany)
The Future of Correlative Imaging
- Andreas Walther (BioImaging Austria)
Thermo Fisher Scientific's Amira Software Workshop
Join Thermo Fisher Scientific on July 15-16 for a workshop on the Thermo Scientific™ Amira™ Software. Their Software Expert will provide you with an introduction to the software, including an interactive hands-on session during which you will be able to perform data visualization, segmentation, quantification and surface generation. This session is meant for new users and beginners.
The workshop will take place at the Francis Crick Institute on:
- Monday 15 July 12.00 - 13.30
- Tuesday 16 July 12.00 - 13.30
To find out more and register for this workshop, click here.
Join Vironova on July 15-16 for workshops on new approaches to sub-visible particle characterization with the MiniTEM System, a compact, low voltage transmission electron microscope. Their application specialist will provide you with an introduction to the MiniTEM software, including automated image acquisition and particle classification to obtain high quality images and accurate quantitative data. The sessions are meant for new and expert users.
The workshop will take place at the Francis Crick Institute on:
- Monday 15 July 12.00 - 13.30 and 15:00 – 15.30
- Tuesday 16 July 12.00 - 13.30 and 15.00 – 15.30
To find out more and register for you 30 mins slot please send an email to email@example.com. We will be in touch to confirm it.
ZEISS ZEN Connect Workshop
- Monday 15 July 12.30 - 13.00
- Tuesday 16 July 12.30 - 13.00
Zeiss will be presenting a 30 minute talk on a new software module which links and references images captured from any source together.
The speaker will be Dr. Alexandra Elli.
Please note, there is an early-bird offer available until Sunday 2 June. After this date, the registration fee will raise by £20.
The symposium will be immediately followed by the 2019 UK Correlative Light and Electron Microscopy Course, from Wednesday 17th to Friday 19th July. The hands-on CLEM course will cover three correlative workflows - 3D CLEM, Integrated Light and EM, and cryo CLEM - using a range of techniques and equipment including Near Infrared Branding, micro CT, Serial Block Face SEM, Focused Ion Beam SEM, high pressure freezing, in-resin fluorescence, ultraLM, integrated light and SEM (SECOM), plunge freezing and cryo-fluorescence. There will also be a CLEM image analysis tutorial session. There are limited places available on the CLEM course.
Please contact Raffaella Carzaniga to express interest.
Location: Francis Crick Institute, London, NW1 1ST
If you are interested in sponsoring this meeting, please contact Kate Jermey.
Agar Scientific. Microscopy, Lab and Medical supplies at your fingertips
A leading international supplier of scientific instruments and accessories for over 40 years, Agar Scientific specialises in consumables and equipment supporting all forms of microscopy.
Agar Scientific provides a fast, efficient online and offline ordering service, allowing customers to purchase with ease from our extensive range of laboratory and medical supplies and equipment.
Our technical support staff has a wealth of experience in specimen preparation and microscopy techniques.
Our online catalogue of microscopy products includes:
TEM Grids - Apertures - Calibration standards - SEM stubs - Specimen preparation equipment - Filaments - Tweezers - Slides & cover glasses - Vacuum coating - Fixatives and resins - EM support films - Magnifiers - Specimen storage
We welcome enquiries regarding the use of any of our products, along with any suggestions for additions to our range.
Orders ship from our UK headquarters in Stansted, Essex, and through our established network of agents and distributors who provide technical and applications support worldwide.
Agar Scientific maintains a documented quality plan specifying our manufacturing and distribution processes and is approved by SGS to ISO9001:2008.
Find out more about Agar Scientific
Web: www.agarscientific.com LinkedIn: Agar Scientific Twitter: @agarscientific
Find out more about Arivis here.
Find out more here.
DELMIC products are focused on high performance, user friendly, integrated, solutions.
Correlative microscopy combines functional information from fluorescence microscopy with structural information from the electron microscope. The SECOM platform integrates a fluorescence microscope with a scanning electron microscope. This combination makes it possible to do fast correlative microscopy with high overlay precision. The system is easy to use for both optical and electron microscopists and makes correlation as simple as drag-and-drop.
Delmic’s second product line provides a platform for novel experiments in nanophotonics. The SPARC system is used for high-performance cathodoluminescence detection. It gives maximum collection efficiency through the precise alignment of the mirror with respect to the electron beam combined with top-of-the-line detectors. This makes it possible to probe structures with very low photon yield. The SPARC system also offers the unique feature of doing angle resolved measurements, enabling momentum spectroscopy.
Learn more about the SECOM and SPARC platforms on the website here.
Gatan is the industry leader in the research, development and manufacturing of SEM and TEM products.
For TEM, Gatan provides an unrivalled range of high performance digital imaging and analytical systems, especially EELS spectrometers and energy filters (EFTEM). For SEM, Gatan are world leaders in cathodoluminescence (CL) technology and complement this with cooling stages and EBIC products.
Gatan is striving for continuous product innovations to increase users' productivity and make electron microscopes more effective and powerful tools. Visit the website for complete information on Gatan's extensive range of products for electron microscopy.
Find out more about Gatan
The ISS Group is a provider of electron optical systems, solutions and related technologies to visualise and characterise materials from the macro down to the nano scale. Whether this is the supply of the latest instrumentation technology or through training and service support of older systems, ISS offers more than 30 years of experience serving the UK and overseas markets. We represent a number of prestigious manufacturers in the UK and Ireland and continue to grow all aspects of the business.
Our friendly and knowledgeable team is available to help you answer your queries.
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Labtech International Ltd
Since 1993 Labtech International has been a leading supplier of innovative instrumentation into the UK and Ireland research markets. The electron microscopy division continues this tradition offering an expanding range of technology-leading instruments, accessories and consumables for SEM, TEM, FIB, LM and probe microscopy.
Our partners include Micro to Nano, who offer an extensive and growing range of microscopy supplies – including SEM stage and stub adaptors. We also represent DiATOME diamond knives, Kleindiek Nanotechnik nanomanipulators, Safematic sputter/carbon coaters, PIE Scientific RF plasma cleaners and from Tousimis fully automatic critical point dryers.
Labtech actively supports its customers through highly qualified teams of field-based sales, service and application specialists.
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Lambda Photometrics Ltd.
Lambda Photometrics Ltd. is a UK distributor specialising in systems and components for Characterisation, Measurement & Analysis. We offer a wide range of products from Fibre Optics, Instrumentation, Metrology, Photonics, Spectroscopy, Machine Vision suppliers as well as Microscopy products. We have experienced and knowledgeable sales and service engineers to support our customers.
Microscopy systems we supply include desktop scanning electron microscopes with EDX from PhenomWorld, Scanning White Light Microscopes from Zygo, hand-held digital microscopes from Scalar, and other visible microscopes.
Microscope components and accessories include confocal microscopy lenses, configurable and zoom systems from Navitar, light sources and illumination from Schott, electro-optic devices from Conoptics, spectroscopy & Raman systems from B&WTek, system design from Zygo, cameras from Baumer, Imperx and Ximea, Software from Norpix and various optical,lighting and image capture products.
Find out more about Lambda Photometrics Ltd.
Leica Microsystems GmbH
Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Ever since the company started as a family business in the nineteenth century, its instruments have been widely recognized for their optical precision and innovative technology. It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes.
Leica Microsystems has seven major plants and product development sites around the world. The company is represented in over 100 countries, has sales and service organizations in 20 countries, and an international network of distribution partners. Its headquarters are located in Wetzlar, Germany.
Find out more about Leica Microsystems
Linkam Scientific Instruments
Linkam develops and manufactures a broad range of heating and freezing stages for both OEM and end users to visualize and explore materials properties. Used in conjunction with light microscopes and other forms of spectroscopy, Linkam stages are found in thousands of laboratories worldwide with the most successful microscope heating stage, the THMS600, selling over 4,000 units alone. Linkam is the market leader in temperature controlled microscopy.
Quorum Technologies are global leaders in electron microscopy (EM) coating and cryogenic preparation providing high performance sample preparation equipment worldwide.
Driven by the demands of the market Quorum specialises in innovating new technology to develop instruments and techniques which have a positive impact on its customers’ lives. Key products in Quorum’s portfolio include the market leading Q Series of vacuum coaters and PP3010T cryo preparation system for SEM and FIB/SEM – market-led genuine ground-up designs that have earned Quorum the respect of the industry and truly set the standard within the specimen preparation field.
Find out more about Quorum Technologies
Find out more about Stratocore
Thermo Fisher Scientific
At Thermo Fisher Scientific, we are committed to accelerating your science by providing a comprehensive suite of solutions for the analysis of cells and their function. Behind this commitment is an incredible team of scientists developing and supporting our innovative instrumentation and products such as the Invitrogen™ Attune™ NxT Flow Cytometer, Invitrogen™ eBioscience™ flow cytometry antibodies, and Invitrogen™ functional reagents. Our flagship products are designed to deliver high-performance results and save you time in the lab. From our extensive flow cytometry cell health reagent portfolio and everyday standards and controls to our Invitrogen™ eBioscience™ Super Bright antibody conjugates, let us help you find unique ways to support discovery of new biological insights.
Find out more about Thermo Fisher Scientific
Find out more about Vironova
Carl Zeiss is an innovative technology leader in the fields of optics, precision engineering and electronic visualisation. Time and time again, we set new, pioneering standards in sophisticated technology for recognising, experiencing, measuring, analysing, structuring and processing a wide spectrum of objects. With professional optics we meet the expectations of even our most critical customers - not only in the fields of research, medicine, industry, but also for use in leisure activities.
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