Cryo Electron Microscopy Course 2020
Course Organiser: Dr Eudri Venter
The popular Cryo Electron Microscopy course will return for 2020, with additional new content not included previously. Covering both theoretical and practical aspects of sample preparation and cryo EM techniques, this residential course is ideal for anyone new to cryo EM, but can also be attended by those wanting to refresh their existing skills.
This intensive five day course sees numerous topic experts covering a variety of sample preparation techniques (including high pressure freezing, rapid cooling and cryo sectioning), as well as cryo-microscopy operation for both scanning and transmission EMs (including specialist techniques such as freeze fracturing and cryo-STEM tomography). Each of the topics will include tutorials and demonstrations, although most the time will go into hands-on practical sessions. The course is aimed at life sciences, however the techniques can be applied to other fields, and we therefore invite participants from any area of academia or industry to attend. We also recommend that participants bring their own samples along, as a large portion of the course is dedicated to giving attendees the opportunity to apply the taught techniques to own research.
Previous course details
- 09:00 Registration
- 10:00 Introductions, Welcome, Course Arrangements
- 10:30 Tea/ Coffee/ Lab Tour
- 11:00 L:HPF-FS
- 12:15 TB: Linkam
- 12:45 Lunch in Cafeteria
Session A: P: HPF-FS
Session B: P: Rapid Cooling
- 15:45 Tea/Coffee
Session A: P: Rapid Cooling
Session B: P: HPF-FS
- 18:00 Fish and Chip Supper
- 09:00 L: Cryoultramicrotomy
Session A: P: Cryosectioning
Session B: P: CryoTEM
- 11:15 Tea/ Coffee
Session A: P: Cryosectioning (Continued)
Session B: P: CryoTEM (Condition)
- 12:45 Lunch in the Cafeteria
Session A: P: CyroTEM
Session B: P: Cryosectioning
- 15:00 Tea/ Coffee
- 15:15 TB: Quorum
Session A: P: CryoTEM (Continued)
Session B: P: Cryosectioning (Continued)
- 17:00 L: Keynote Lecture - New Advances in Cryo-EM
- 18:00 Free Evening
- 09:00 L: CryoSEM
- 10:00 Tea/ Coffee
- 10:15 L: CryoTEM
Session A: P: CryoSTEM Tomography
Session B: P: CryoSEM
- 12:30 Lunch in the Cafeteria
Session A: CryoSTEM Tomography (Continued)
Session B: P:CryoSEM (Continued)
- 14:45 TB: Gatan
Session A: P: CryoSEM
Session B: P: CryoSTEM Tomography
- 16:30 Tea/ Coffee
Session A: P: CryoSEM (Continued)
Session B: P: CryoSTEM Tomography (Continued)
- 18:00 Free Evening
- 09:00 P: Supervised Practice with Provided or Your Own Samples
- 11:00 Tea/ Coffee/ Photo
- 11:30 P: Supervised Practice with Provided or Your Own Samples
- 12:45 Lunch in the Cafeteria
- 13:45 L: Plenary Lecture - Cryo-STEM Tomography
- 15:00 P: Supervised Practice with Provided or Your Own Samples
- 15:45 Tea/ Coffee
- 16:00 TB: JEOL UK
- 16:30 P: Supervised Practice with Provided or Your Own Samples
- 18:00 Luton Hoo Dinner
- 09:00 L: Common Artefacts
- 09:30 P: Review Results with Delegate Participation and Open Discussion
- 11:00 Tea/ Coffee
- 11:15 L: Presentation of Certificates
Dr Eudri Venter
Eudri Venter completed his PhD in viral genetics at the University of Pretoria (UP), South Africa in 2014, focusing on applying an integrated microscopy approach towards studying virus morphogenesis in different cell types. Following this, and a companion post-doc, he joined the Laboratory for Microscopy and Microanalysis at UP, where he worked in a versatile research environment. His areas of expertise include scanning and transmission electron microscopy; light, widefield fluorescence and confocal/super resolution microscopy, as well as micro analytical techniques such as energy dispersive x-ray spectroscopy, electron backscatter diffraction and atomic force microscopy. Currently he is the resident electron microscopist at Rothamsted Research Bioimaging, applying a multidisciplinary approach to a variety of agricultural research areas.
Mr Dennis Fitzpatrick
Dr Roland Fleck
King's College London
Roland is Reader and Director of the King's Centre for Ultra Structural Imaging
Mr Gareth Jackson
Gareth started his career in a clinical EM department in the early nineties. Since then he has worked for Olympus Microscopes and Leica Microsystems. Gareth is an active member of the EM committee and has been involved in many RMS courses over the years including the EM Summer School, Rothamsted Research Cryo Course and the Cell Imaging Techniques course at Oxford Brookes University.
Dr Smita Kurup
Mrs Judith Mantell
University of Bristol
Judith Mantell is the Electron Microscopy Specialist for the Wolfson Bioimaging Facility at the University of Bristol, working with Dr Paul Verkade. The facility houses 2 TEMs, complete with cryo facilities and a FEG SEM with cryo attachment. Although starting out as a Physics graduate and working in materials research, Judith has since worked for Oxford Instruments as Sales and Applications specialist in their EM division (now owned by Gatan), and previously for Philips Electron Optics (now FEI) as a TEM Application specialist both in Eindhoven and Cambridge.
Dr Kent McDonald
University of California, Berkeley
Kent McDonald received his Ph.D. in Botany from the University of California, Berkeley in 1972. Following a year of teaching at UCLA, he took a Post-doctoral position with J.D. Pickett-Heaps at the University of Colorado, Boulder to study the ultrastructure of mitosis in algae. In 1975, he joined the laboratory of J.R. McIntosh in Boulder to re-focus his research on the cell biology of mitosis. This led him back to Berkeley in 1979 to work on correlative light and electron microscopy of PtK cells and diatoms in the lab of W.Zacheus Cande. In 1987 he returned to Boulder to join the High Voltage EM Lab begun by Keith Porter and subsequently run by J.R. McIntosh. It was at this time that the importance of cryofixation by high pressure freezing became a compelling interest that persists to this day. In 1993 he moved back to Berkeley to assume the Directorship of the campus Electron Microscope Laboratory, a teaching and research resource for UC Berkeley and other Bay Area institutions. Kent retired from UC Berkeley in 2017. His current research interest is improving the procedures for biological specimen preparation. Starting with high pressure frozen material he has recently developed methods for freeze substitution and subsequent resin embedding that takes only 6 hours or less to complete using simple, inexpensive equipment. Work to improve on-section immunolabeling efficiency is another recent activity.
Dr Sharon Wolf
Weizmann Institute of Science
Sharon Grayer Wolf is a Senior Research Fellow and Head of the Electron Microscopy Unit at the Weizmann Institute of Science in Rehovot, Israel. Sharon received her MSc and PhD under Prof. Leslie Leiserowitz at the Weizmann Institute, studying lipid systems at the air-water interface by surface X-ray techniques. Sharon performed her postdoctoral training with the late Dr. Kenneth H Downing at Lawrence Berkeley Laboratories, and together with fellow postdoc Eva Nogales, solved the first high resolution structure of tubulin by electron crystallography. Sharon returned to the Weizmann Institute in 1998. In 2014, Sharon and collaborators Prof. Michael Elbaum and Dr. Lothar Houben introduced the technique of cryo-STEM tomography (CSTET) and in-situ chemical characterization of vitrified cells. Sharon is continuing development of CSTET as a tool for structural cell biology. Sharon was recently awarded with the Shimon and Golde Picker Endowed Chair in Advanced Electron Microscopy.
Mr Andy Yarwood
If you are interested in sponsoring this course, please contact Debbie Hunt.
Gatan is the industry leader in the research, development and manufacturing of SEM and TEM products.
For TEM, Gatan provides an unrivalled range of high performance digital imaging and analytical systems, especially EELS spectrometers and energy filters (EFTEM). For SEM, Gatan are world leaders in cathodoluminescence (CL) technology and complement this with cooling stages and EBIC products.
Gatan is striving for continuous product innovations to increase users' productivity and make electron microscopes more effective and powerful tools. Visit the website for complete information on Gatan's extensive range of products for electron microscopy.
Find out more about Gatan
JEOL (UK) Ltd
JEOL is proud to be celebrating over 60 years of high end instrument sales and support worldwide.
JEOL UK offer sales, support and applications training from our office in Welwyn Garden City, Hertfordshire.
Our instrumentation includes scanning electron microscopes (SEM), scanning probe microscopes, & transmission electron microscopes (TEM). As one of the world’s leading suppliers of scientific equipment, we are proud to offer our customers the highest level of sales and technical support maximising their investment in JEOL products.
Find out more about Jeol UK
Linkam Scientific Instruments
Linkam develops and manufactures a broad range of heating and freezing stages for both OEM and end users to visualize and explore materials properties. Used in conjunction with light microscopes and other forms of spectroscopy, Linkam stages are found in thousands of laboratories worldwide with the most successful microscope heating stage, the THMS600, selling over 4,000 units alone. Linkam is the market leader in temperature controlled microscopy.
Quorum Technologies are global leaders in electron microscopy (EM) coating and cryogenic preparation providing high performance sample preparation equipment worldwide.
Driven by the demands of the market Quorum specialises in innovating new technology to develop instruments and techniques which have a positive impact on its customers’ lives. Key products in Quorum’s portfolio include the market leading Q Series of vacuum coaters and PP3010T cryo preparation system for SEM and FIB/SEM – market-led genuine ground-up designs that have earned Quorum the respect of the industry and truly set the standard within the specimen preparation field.
Find out more about Quorum Technologies
Book a place
- £1295 Member rate
- £1770 Member rate Incl. Accommodation
- £1395 Non-Member rate
- £1870 Non-member rate Incl. Accommodation
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Location University of Sheffield