Cryo EM Course 2018
The popular course in Cryo Electron Microscopy will return for 2018. Using both lectures and practical demonstrations, this residential course is ideal for anyone new to Cryo EM or just needing to brush up on their existing skills.
This intensive five day course covers sample preparation and cryo-microscopy techniques for both scanning and transmission electron microscopy, and is comprised of tutorials, demonstrations and hands on practical sessions. The course is aimed at life sciences however the techniques can be applied to other fields so participants from any area of academia or industry who are interested in learning cryo-microscopy skills or updating their knowledge are invited to attend. During the course you will have the opportunity to practise the techniques on your own samples so we highly recommend bringing some samples relevant to your research.
Online registration has now closed as the course is full.
If you would like to be added to a waiting list, please email Debbie Hunt.
- 19:00Welcome dinner at Prezzo
- 10:00Introductions and Welcome
- 10:15Course arrangements / AOB - Rebecca Lauder
- 10:30Tea and Coffee Break
- 10:45High Pressure Freezing - Kent McDonald
- 12:00Tour of the BioImaging Unit - Rebecca Lauder
- 13:30Cryo Stage Demonstration
- 14:30High Pressure Freezing
- 16:00Tea and Coffee Break
- 16:15Freeze Substitution - Adrian Hughes
- 17:45End of Day
- 18:00Pub Quiz and Fish and Chip Supper
- 09:00Cryoultramicrotomy - 0 Gareth Jackson
- 10:00Cryosectioning - Gareth Jackson
- 11:00Tea and Coffee Break
- 11:15Cryosectioning (Continued) - Gareth Jackson
- 14:00Rapid Cooling - James Streetley
- 15:15Tea and Coffee Break
- 15:30Techno Bite
- 16:00Rapid Cooling (Continued) - James Streetley
- 17:00Keynote Speech - Bram Koster
- 18:00End of Day
- 09:00CryoSEM - Judith Mantell
- 10:00Tea and Coffee Break
- 10:30CryoTEM - James Streetley
- 11:30CryoTEM applications - James Streetley
- 13:30CryoTEM applications (Continued) - James Streetley
- 15:00CryoSEM applications - Andy Yarwood, Judith Mantell and Dave Bhella
- 15:45Tea and Coffee Break
- 16:00CryoSEM applications (Continued) - Andy Yarwood, Judith Mantell and Dave Bhella
- 18:00End of Day
- 09:00Supervised practice - All Lecturers
- 11:00Tea and Coffee Break
- 11:30Supervised practice with provided or own samples - All Lecturers
- 13:45Plenary Lecture - Kent McDonald
- 15:00Supervised practice with provided or own samples (Continued) - All Lecturers
- 15:45Tea and Coffee Break
- 16:00Techno Bite
- 16:30Supervised practice with provided or own samples (Continued) - All Lecturers
- 17:30End of day
- 19:00Course Dinner at the Luton Hoo
- 09:00Common Artefacts - Rebecca Lauder
- 09:30Review results with delegate participation and open discussion - All Lecturers
- 11:00Tea and Coffee Break
- 11:15Presentation of Certificates - Rebecca Lauder
- 11:45End of Course
Dr Rebecca Lauder
Rebecca Lauder is the electron microscopist in Rothamsted Research Bioimaging. She obtained her PhD in 2011 from the University of Glasgow under the supervision of Dr David Bhella. Following her PhD she joined Dr Frazer Rixon’s group at the MRC-University of Glasgow Centre for Virus Research as a post doc working on characterising the structure and interactions of Herpes Simplex Virus 1 inside whole cells. This involved a variety of techniques including cryoEM, cryo tomography, HVEM, and correlative light-electron microscopy. In 2015 she started at Rothamsted where she provides EM imaging for a variety of projects as well as training users in EM and sample preparation techniques
Prof Bram Koster
Leiden University Medical Center
The research and method developments of the Koster group are focused on the development of methods combining high resolution fluorescence light-microscopy with ultra-structural imaging with electron microscopy. His group develops methods and technologies enabling the identification and visualization of specific macromolecular arrangements and/or changes in the cellular context. Bram Koster is one of the pioneers in the development of 3D electron microscopic imaging using electron tomography and worked on life sciences and materials sciences applications. He obtained his PhD Applied Physics in 1989 (Delft University, the Netherlands). After post-doctoral positions at UCSF (San Francisco) and the Max-Planck-Institute for Biochemistry (Martinsried, Germany) he returned to the Netherlands in 1997 (Utrecht University). In 2006 he relocated to his current position at the Leiden University Medical Center in the In 2014 he was appointed director of the Netherlands Center of Electron Nanoscopy (NeCEN), also in Leiden.
Dr James Streetley
James Streetley is a post-doctoral research assistant in Dr David Bhella’s group at the MRC-University of Glasgow Centre for Virus Research. His research uses cryo-EM to image the replication machinery of respiratory syncytial virus and he maintains the EM facilities at the CVR, supporting other research projects requiring cryo-EM and tomography. In April, these facilities will become part of The Scottish Centre for Macromolecular Imaging as we take delivery of a new 300kV automated cryo-microscope.
James has been using cryo-EM since he was a masters student with Prof Elena Orlova at Birkbeck College, London before moving across the city for a PhD with Dr Peter Rosenthal at the MRC National Institute for Medical Research doing cryo-electron tomography of vitrified endothelial cells. He has been in Glasgow since January 2015.
Dr Kent McDonald
University of California, Berkeley
McDonald received his Ph.D. in Botany from the University of California, Berkeley in 1972. Following a year of teaching at UCLA, he took a Post-doctoral position with J.D. Pickett-Heaps at the University of Colorado, Boulder to study the ultrastructure of mitosis in algae. In 1975, he joined the laboratory of J.R. McIntosh in Boulder to re-focus his research on the cell biology of mitosis. This led him back to Berkeley in 1979 to work on correlative light and electron microscopy of PtK cells and diatoms in the lab of W.Zacheus Cande. In 1987 he returned to Boulder to join the High Voltage EM Lab begun by Keith Porter and subsequently run by J.R. McIntosh. It was at this time that the importance of cryofixation by high pressure freezing became a compelling interest that persists to this day. In 1993 he moved back to Berkeley to assume the Directorship of the campus Electron Microscope Laboratory, a teaching and research resource for UC Berkeley and other Bay Area institutions. His current research interest is improving the procedures for biological specimen preparation. Starting with high pressure frozen material he has recently developed methods for freeze substitution and subsequent resin embedding that takes only 6 hours or less to complete using simple, inexpensive equipment. Work to improve on-section immunolabeling efficiency is another recent activity.
Mr Gareth Jackson
Gareth started his career in a clinical EM department in the early nineties. Since then he has worked for Olympus Microscopes and Leica Microsystems. Gareth is an active member of the committee and has been involved in many RMS courses over the years including the EM Summer School, Rothamsted Research Cryo Course and the Cell Imaging Techniques course at Oxford Brookes University.
Mr Andy Yarwood
Mr Dennis Fitzpatrick
Venue for the course
The Cryo Electron Microscopy Course takes place at Rothamsted Research. The full address is West Common, Harpenden, AL5 2JQ. For more information about Rothamsted Research, please visit www.rothamsted.ac.uk.
Transport to the venue and accommodation
If you are flying to the UK, we recommend that you travel to Luton Airport.
The accommodation package includes 5 nights’ en-suite, bed and breakfast accommodation.
Breakfast will be provided as part of the accommodation package, including on the morning of check out.
All buffet lunches, tea / coffee and biscuits are included in your registration fee. The evening meals will be held in the following locations:
- Sunday’s evening meal will be held at a restaurant in St Albans. (for delegates that have selected the accommodation package).
- Monday’s evening meal will be a fish and chip supper, followed by a pub quiz at the Pavilion at Rothamsted Research (all delegates and speakers are invited to attend).
- Tuesday there is no evening meal provided, delegates have a free evening.
- Wednesday there is no evening meal provided, delegates have a free evening.
- Thursday’s evening meal will be held at the Luton Hoo Hotel and Golf Club (all delegates and speakers are invited to attend).
Please note that Tuesday and Wednesday are free evenings, where no evening meals are provided.
Registration will be taking place on Monday 18 April from 09.00 – 09.15 in the foyer of Rothamsted Research.
Dietary and Access requirements
The RMS is committed to our delegate’s health and wellbeing. Therefore if you have any dietary or access requirements or questions please contact Debbie Hunt.
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