Cryo EM Course 2018
The popular course in Cryo Electron Microscopy will return for 2018. Using both lectures and practical demonstrations, this residential course is ideal for anyone new to Cryo EM or just needing to brush up on their existing skills.
This intensive five day course covers sample preparation and cryo-microscopy techniques for both scanning and transmission electron microscopy, and is comprised of tutorials, demonstrations and hands on practical sessions. The course is aimed at life sciences however the techniques can be applied to other fields so participants from any area of academia or industry who are interested in learning cryo-microscopy skills or updating their knowledge are invited to attend. During the course you will have the opportunity to practise the techniques on your own samples so we highly recommend bringing some samples relevant to your research.
Online registration has now closed as the course is full.
If you would like to be added to a waiting list, please email Debbie Hunt.
- 19:00 Welcome dinner at Prezzo
- 09:00 Registration
- 10:00 Introductions and Welcome
- 10:15 Course arrangements / AOB - Rebecca Lauder
- 10:30 Tea and Coffee Break
- 10:45 High Pressure Freezing - Kent McDonald
- 12:00 Tour of the BioImaging Unit - Rebecca Lauder
- 12:30 Lunch
- 13:30 Cryo Stage Demonstration
- 14:30 High Pressure Freezing
- 16:00 Tea and Coffee Break
- 16:15 Freeze Substitution - Adrian Hughes
- 17:45 End of Day
- 18:00 Pub Quiz and Fish and Chip Supper
- 09:00 CryoSEM - Judith Mantell
- 10:00 Tea and Coffee Break
- 10:30 CryoTEM - James Streetley
- 11:30 CryoTEM applications - James Streetley
- 12:30 Lunch
- 13:30 CryoTEM applications (Continued) - James Streetley
- 15:00 CryoSEM applications - Andy Yarwood, Judith Mantell and Dave Bhella
- 15:45 Tea and Coffee Break
- 16:00 CryoSEM applications (Continued) - Andy Yarwood, Judith Mantell and Dave Bhella
End of Day
- 09:00 Supervised practice - All Lecturers
- 11:00 Tea and Coffee Break
- 11:30 Supervised practice with provided or own samples - All Lecturers
- 12:45 Lunch
- 13:45 Plenary Lecture - Kent McDonald
- 15:00 Supervised practice with provided or own samples (Continued) - All Lecturers
- 15:45 Tea and Coffee Break
- 16:00 Techno Bite
- 16:30 Supervised practice with provided or own samples (Continued) - All Lecturers
- 17:30 End of day
- 19:00 Course Dinner at the Luton Hoo
- 09:00 Common Artefacts - Rebecca Lauder
- 09:30 Review results with delegate participation and open discussion - All Lecturers
- 11:00 Tea and Coffee Break
- 11:15 Presentation of Certificates - Rebecca Lauder
- 11:45 End of Course
Dr Rebecca Lauder
Rebecca Lauder is the electron microscopist in Rothamsted Research Bioimaging. She obtained her PhD in 2011 from the University of Glasgow under the supervision of Dr David Bhella. Following her PhD she joined Dr Frazer Rixon’s group at the MRC-University of Glasgow Centre for Virus Research as a post doc working on characterising the structure and interactions of Herpes Simplex Virus 1 inside whole cells. This involved a variety of techniques including cryoEM, cryo tomography, HVEM, and correlative light-electron microscopy. In 2015 she started at Rothamsted where she provides EM imaging for a variety of projects as well as training users in EM and sample preparation techniques
Prof Bram Koster
Leiden University Medical Center
The research and method developments of the Koster group are focused on the development of methods combining high resolution fluorescence light-microscopy with ultra-structural imaging with electron microscopy. His group develops methods and technologies enabling the identification and visualization of specific macromolecular arrangements and/or changes in the cellular context. Bram Koster is one of the pioneers in the development of 3D electron microscopic imaging using electron tomography and worked on life sciences and materials sciences applications. He obtained his PhD Applied Physics in 1989 (Delft University, the Netherlands). After post-doctoral positions at UCSF (San Francisco) and the Max-Planck-Institute for Biochemistry (Martinsried, Germany) he returned to the Netherlands in 1997 (Utrecht University). In 2006 he relocated to his current position at the Leiden University Medical Center in the In 2014 he was appointed director of the Netherlands Center of Electron Nanoscopy (NeCEN), also in Leiden.
Dr James Streetley
James Streetley is a post-doctoral research assistant in Dr David Bhella’s group at the MRC-University of Glasgow Centre for Virus Research. His research uses cryo-EM to image the replication machinery of respiratory syncytial virus and he maintains the EM facilities at the CVR, supporting other research projects requiring cryo-EM and tomography. In April, these facilities will become part of The Scottish Centre for Macromolecular Imaging as we take delivery of a new 300kV automated cryo-microscope.
James has been using cryo-EM since he was a masters student with Prof Elena Orlova at Birkbeck College, London before moving across the city for a PhD with Dr Peter Rosenthal at the MRC National Institute for Medical Research doing cryo-electron tomography of vitrified endothelial cells. He has been in Glasgow since January 2015.
Dr Kent McDonald
University of California, Berkeley
McDonald received his Ph.D. in Botany from the University of California, Berkeley in 1972. Following a year of teaching at UCLA, he took a Post-doctoral position with J.D. Pickett-Heaps at the University of Colorado, Boulder to study the ultrastructure of mitosis in algae. In 1975, he joined the laboratory of J.R. McIntosh in Boulder to re-focus his research on the cell biology of mitosis. This led him back to Berkeley in 1979 to work on correlative light and electron microscopy of PtK cells and diatoms in the lab of W.Zacheus Cande. In 1987 he returned to Boulder to join the High Voltage EM Lab begun by Keith Porter and subsequently run by J.R. McIntosh. It was at this time that the importance of cryofixation by high pressure freezing became a compelling interest that persists to this day. In 1993 he moved back to Berkeley to assume the Directorship of the campus Electron Microscope Laboratory, a teaching and research resource for UC Berkeley and other Bay Area institutions. His current research interest is improving the procedures for biological specimen preparation. Starting with high pressure frozen material he has recently developed methods for freeze substitution and subsequent resin embedding that takes only 6 hours or less to complete using simple, inexpensive equipment. Work to improve on-section immunolabeling efficiency is another recent activity.
Mr Gareth Jackson
Gareth started his career in a clinical EM department in the early nineties. Since then he has worked for Olympus Microscopes and Leica Microsystems. Gareth is an active member of the committee and has been involved in many RMS courses over the years including the EM Summer School, Rothamsted Research Cryo Course and the Cell Imaging Techniques course at Oxford Brookes University.
Mr Andy Yarwood
Mr Dennis Fitzpatrick
Venue for the course
The Cryo Electron Microscopy Course takes place at Rothamsted Research. The full address is West Common, Harpenden, AL5 2JQ. For more information about Rothamsted Research, please visit www.rothamsted.ac.uk.
Transport to the venue and accommodation
If you are flying to the UK, we recommend that you travel to Luton Airport.
The accommodation package includes 5 nights’ en-suite, bed and breakfast accommodation.
Breakfast will be provided as part of the accommodation package, including on the morning of check out.
All buffet lunches, tea / coffee and biscuits are included in your registration fee. The evening meals will be held in the following locations:
- Sunday’s evening meal will be held at a restaurant in St Albans. (for delegates that have selected the accommodation package).
- Monday’s evening meal will be a fish and chip supper, followed by a pub quiz at the Pavilion at Rothamsted Research (all delegates and speakers are invited to attend).
- Tuesday there is no evening meal provided, delegates have a free evening.
- Wednesday there is no evening meal provided, delegates have a free evening.
- Thursday’s evening meal will be held at the Luton Hoo Hotel and Golf Club (all delegates and speakers are invited to attend).
Please note that Tuesday and Wednesday are free evenings, where no evening meals are provided.
Registration will be taking place on Monday 18 April from 09.00 – 09.15 in the foyer of Rothamsted Research.
Dietary and Access requirements
The RMS is committed to our delegate’s health and wellbeing. Therefore if you have any dietary or access requirements or questions please contact Debbie Hunt.
Diamond Knives - Development, Manufacturing & Customer Service since 1970.
We offer technical assistance in all fields of ultramicrotomy, free sectioning tests for all types of samples, many years of experience in perfecting our knives. With a telephone call or an email we can inform you of any details you require. We are convinced that our future developments will allow diamond knives to be used for many new applications.
Gatan is the industry leader in the research, development and manufacturing of SEM and TEM products.
For TEM, Gatan provides an unrivalled range of high performance digital imaging and analytical systems, especially EELS spectrometers and energy filters (EFTEM). For SEM, Gatan are world leaders in cathodoluminescence (CL) technology and complement this with cooling stages and EBIC products.
Gatan is striving for continuous product innovations to increase users' productivity and make electron microscopes more effective and powerful tools. Visit the website for complete information on Gatan's extensive range of products for electron microscopy.
JEOL (UK) Ltd
JEOL is proud to be celebrating over 60 years of high end instrument sales and support worldwide.
JEOL UK offer sales, support and applications training from our office in Welwyn Garden City, Hertfordshire.
Our instrumentation includes scanning electron microscopes (SEM), scanning probe microscopes, & transmission electron microscopes (TEM). As one of the world’s leading suppliers of scientific equipment, we are proud to offer our customers the highest level of sales and technical support maximising their investment in JEOL products.
Leica Microsystems GmbH
Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Ever since the company started as a family business in the nineteenth century, its instruments have been widely recognized for their optical precision and innovative technology. It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes.
Leica Microsystems has seven major plants and product development sites around the world. The company is represented in over 100 countries, has sales and service organizations in 20 countries, and an international network of distribution partners. Its headquarters are located in Wetzlar, Germany.
Linkam Scientific Instruments
Linkam develops and manufactures a broad range of heating and freezing stages for both OEM and end users to visualize and explore materials properties. Used in conjunction with light microscopes and other forms of spectroscopy, Linkam stages are found in thousands of laboratories worldwide with the most successful microscope heating stage, the THMS600, selling over 4,000 units alone. Linkam is the market leader in temperature controlled microscopy.
Quorum Technologies are global leaders in electron microscopy (EM) coating and cryogenic preparation providing high performance sample preparation equipment worldwide.
Driven by the demands of the market Quorum specialises in innovating new technology to develop instruments and techniques which have a positive impact on its customers’ lives. Key products in Quorum’s portfolio include the market leading Q Series of vacuum coaters and PP3010T cryo preparation system for SEM and FIB/SEM – market-led genuine ground-up designs that have earned Quorum the respect of the industry and truly set the standard within the specimen preparation field.