Frontiers in BioImaging 2018
Scientific Organisers: Susan Cox, King's College London; Sian Culley, University College London; Gail McConnell, University of Strathclyde; Brian Patton, University of Strathclyde; Alex Sossick, University of Cambridge and Imogen Sparkes, University of Bristol
Frontiers in BioImaging 2018 will focus on the latest developments in applications of optical microscopy, mesoscopy and image analysis across a range of biological fields. Sessions will cover technical developments and applications of these microscopy-based approaches to key cell and molecular biology questions. The meeting will cover the key challenges in microscopy today: super-resolution imaging, phototoxicity and light sheet based methods, detection of in situ protein interactions and new tools for fluorescence visualisation and analysis.
This is an ideal meeting for both new and established researchers to engage with a broad range of imaging approaches and to make valuable contacts with leading groups in the field. There will be an exhibition held alongside this meeting, where the tea, coffee, lunch and posters will all be held. We are very grateful to the exhibitors and their support.
Registration has now closed for this meeting.
- 09:30 Registration, Tea and Coffee
- 10:30Welcome and Introduction
- 10:40 Session One
Imaging actin dynamics and interactions - Maddy Parsons, King's College London
- 11:10Can the measurement of fluorescence lifetime impact on the lifetime of cancer patients? - Paul Barber, UCL Cancer Institute
- 11:25 Invited Speaker:
Elastic properties of fibrous proteins and tissues probed Brillouin microspectroscopy - Francesca Palombo, University of Exeter
- 11:55Development of a high speed fluorescence lifetime imaging system for pathological screening - Simon Poland, King's College London
- 12:10 Flash Talk:
sCMOS technology in life sciences - Photon Lines
- 12:15RMS Light Microscopy Section Annual General Meeting
- 12:30 Lunch, Exhibition and Posters
- 13:45 Session Two:
Democratising live-cell high-speed super-resolution microscopy - Ricardo Henriques, University College London
- 14:15Graphene Oxide Films Provide 10-100 Contrast Enhancement Factors in Super-Resolution Fluorescence Imaging (STORM) - Thomas Waigh, University of Manchester
- 14:30Single-molecule light-sheet and 3D super-resolution imaging of resting lymphocytes - Aleks Ponjavic, University of Cambridge
- 14:45Diamond embedded electrospun nanofibers for widefield quantum sensing in biological systems - Joshua Price, Keele University
- 15:00 Flash Talk:
Fast, Lifetime Contrast Imaging with Leica SP8 FALCON - Leica Microsystems
- 15:05 Tea, Coffee, Exhibition and Posters
- 15:35 Session Three:
Strategies for mesoscale imaging of non-transparent samples- Sebastian Munck, VIB Bio Imaging Core KU Leuven
- 16:05Acousto Optic Lens Microscopy and its extension to large area 2-photon microscopy for Neuroscience applications - Antoine Valera, University College London
- 16:20Clearing the Haze A Fast Optical Sectioning Method for Widefield Mesoscopy on the Mesolens Using HiLo Microscopy - Jan Schniete, University of Strathclyde
- 16:35 Flash Talk:
The development of scientific CMOS cameras for low light and large field of view imaging - Photometrics UK
- 16:40 Keynote Speaker:
Multiphoton imaging across millimeter length scales with subcellular and subsecond resolution - Spencer Smith, University of North Carolina- Chapel Hill
- 17:25 Drinks, Posters and Exhibition
- 19:00 Conference Dinner at the Drygate
- 09:30 Session Four:
In vivo STED microscopy - Katrin Willig, University Medical Center Göttingen
- 10:00Development of photo-acoustics based microscopy for in vivo and in vitro studies of biological models- Elena Tcarenkova, University of Turku
- 10:15Unraveling the nanoscale architecture of podosomes by combined fluorescence super-resolution and atomic force microscopy - Liisa Hirvonen, King's College London
- 10:30Super-resolution solid immersion lens single molecule localisation microscopy: from room to cryogenic temperature - Lin Wang, Rutherford Appleton Laboratory
- 10:45 Tea, Coffee, Exhibition and Posters
- 11:15 Session Five:
How chromatin organises in mammalian cells - lessons from 3D super-resolution microscopy - Lothar Schermelleh, University of Oxford
- 11:45Artefact free high density localisation microscopy - Richard Marsh, King's College London
- 12:00Environmental Microscopy for Plant Root Phenotyping - Mike MacDonald, University of Dundee
- 12:15Tracking electroporated proteins in 3D within bacteria - Helen Miller, University of Oxford
- 12:30The hard X-ray Nanoprobe at Diamond Light Source - Fernando Cacho-Nerin, Diamond Light Source
- 12:45 Lunch, Exhibition and Posters
- 14:00 Session Six:
Quantitative imaging of structure and dynamics in biological transport networks - Mark Fricker, University of Oxford
- 14:30The encapsulation of conjugated polymers for use as biological imaging agents - Struan Bourke, King's College London
- 14:45Hybrid Optical Gating Allows 4D Imaging and Quantification in the Developing Zebrafish Heart - Chas Nelson, University of Glasgow
- 15:00 Flash Talk:
Appropriate Dichroic Selection - Laser 2000
- 15:05Lower cost, open source optical projection tomography and enhanced performance using structured illumination - Samuel Davis, Imperial College London
- 15:20Light sheet microscopy for high content 3-D imaging of 3-D tissue cultures in a 96-well plate format - Hugh Sparks , Imperial College London
- 15:35Concluding Remarks
- 15:45 Conference Close
The winners of the poster prize competition, sponsored by Zeiss, are as follows:
- 1st Prize - Conor Treacy, King's College London - Mechanosensitive protein-protein interactions in nascent focal adhesions determined by FRET sensing using multiphoton fluorescence lifetime imaging.
- 2nd Prize - Liam Rooney, University of Strathclyde - Applying the Mesolens to Microbiology Investigating the Structural Organisation of Bacterial Biofilms
From confocal microscope to virtual reality; technical and educational considerations – Craig Daly, University of Glasgow
The spatial distribution of cardiac ryanodine receptor phosphorylation investigated using super-resolution microscopy – Carl Harrison, University of Exeter
Characterisation and calibration of a 3D super-resolution microscopy system – Lucas Herdly, University of Strathclyde
Nanodiamonds and adaptive optics for deep tissue super-resolution microscopy – Graeme Johnstone, University of Strathclyde
Single-Molecule Instantaneous Velocity Measurement with a sCMOS Camera Operated in the Ultrafast Double-Exposure Mode – Mirella Koleva, King’s College London
Attenuation-compensation techniques for overcoming losses in light-sheet microscopy – Jonathan Nylk, University of St Andrews
New insights into one/two-photon properties of mScarlet fluorescent protein, its versatile use in living and fixed cells and tissues and in organoids – Ralf Palmisano, OICE
Applying the Mesolens to Microbiology Investigating the Structural Organisation of Bacterial Biofilms – Liam Rooney, University of Strathclyde
Elucidation of Bacterial Motility Dynamics using Interference Reflection Microscopy - Liam Rooney, University of Strathclyde
A computational method for extracting 3D information from standing wave images of red blood cells – Ross Scrimgeour, University of Strathclyde
The development of an OPTIMUM microscope for the applications in cellular biology and life sciences – Tiehan Shen, University of Salford
Development of a flexible light sheet fluorescence microscope for cardiac cell imaging applications – Hugh Sparks, Imperial College
Automated interpretation of time-lapse quantitative phase image by machine learning – Lenka Štrbková – Central European Institute of Technology
Standing wave microscopy of red blood cell membrane morphology with high temporal resolution – Peter William Tinning, University of Strathclyde
Mechanosensitive protein-protein interactions in nascent focal adhesions determined by FRET sensing using multiphoton fluorescence lifetime imaging – Conor Treacy, King’s College London
Entertaining (with) E. coli O157 - Kath Wright, The James Hutton Institute
Democratising live-cell high-speed super-resolution microscopy
Dr Ricardo Henriques
University College London
Dr Ricardo Henriques is a group leader since 2013 at both the University College London and Francis Crick Institute in the UK. His group undergoes research in optical and computational biophysics, with a special interest in super-resolution microscopy and host-pathogen interactions. He graduated in Physics, specialising in biophotonics and robotics. He finished his PhD In 2011 on the topic of advancing super-resolution microscopy technologies (Musa Mhlanga lab). He then pursued postdoc research at Institut Pasteur Paris, studying HIV-1 T-cell infection through nanoscale imaging (Christophe Zimmer lab).
Quantitative imaging of structure and dynamics in biological transport networks
Dr Mark Fricker
Department of Plant Sciences, University of Oxford
Mark Fricker started as a plant physiologist with Colin Willmer in Stirling on dissecting signal transduction pathways in stomatal physiology, and then quantitative imaging of Ca2+ in Edinburgh with Tony Trewavas and Nick Read. He continued with in vivo imaging of Ca2+, pH and redox dynamics in plant and then fungal systems after the move to Oxford sometime last century, which evolved into the current interest in signalling and transport in networked systems, and an IgNobel prize in 2010. Experimental investigations cover a range of scales including confocal ratio imaging on a micron scale, radiolabel scintillation imaging at an intermediate scale, and network analysis and mathematical modelling to predict behaviour across all scales. As part of this work, he has been developing image analysis methods to quantify network architecture, dynamics and internal flows at different organisational scales, including sub-cellular ER networks, and macroscopic networks, such as fungi, slime molds, and leaf veins. The resultant fully-weighted network graphs then provide the input to predictive biophysical models to probe the mechanisms leading to the emergence of self-organised, adaptive behaviour.
Strategies for mesoscale imaging of non-transparent samples
Dr Sebastian Munck
VIB Bio Imaging Core KU Leuven
I started my carrer in Munich, where I studied Biology and later obtained a Ph.D at the BioImaging Center of the Ludwig-Maximilians-University. Later I worked as a Product Manager for Till Photonics, in Germany, between 2003 and 2004. After that I moved to Innsbruck as a Postdoc at the Medical University, from 2004 to 2006. I became Staff Scientist at the VIB in 2007 and in 2013 consequently independent group leader as Expert Technologist. In this function I also established the Leuven part of the VIB Bio Imaging Core and the Departmental imaging facility at the Center for Brain and Disease research. I was appointed Assistant Professor (part-time) at Faculty of Medicine KU Leuven in 2015.
Elastic properties of fibrous proteins and tissues probed Brillouin microspectroscopy
Dr Francesca Palombo
University of Exeter
My research is focused on the development of FTIR, Raman and Brillouin spectroscopy methods for applications to the biomedical sciences. I am particularly interested in the physical and chemical aspects of biological systems at a molecular level, as well their implications in disease. Previously, I developed the application of ATR-FTIR imaging to atherosclerosis in small animal models. I applied both ultrafast OHD-OKE and THz Raman scattering to elucidate the dynamics, structure and interactions in ionic solutions. My PhD was focused on H-bonding properties of octanols from the liquid to supercritical fluid phase using FTIR and Raman spectroscopy.
Imaging actin dynamics and interactions
Prof Maddy Parsons
RMS Honorary Secretary Biological Science
King's College London
Maddy Parsons is Professor of Cell Biology at King’s College London. Maddy completed her PhD in Biochemistry within the Department of Medicine at University College London in 2000. During her PhD she analysed the role of mechanical forces in dermal scarring. She then moved to Cancer Research UK laboratories in London for a 4-year postdoctoral position where she used advanced microscopy techniques including FRET/FLIM to dissect adhesion receptor signaling to the actin cytoskeleton and how this controlled directed cell invasion. Based on these achievements, Maddy was awarded a Royal Society University Research Fellowship in 2005 to establish her own group within the Randall Division of Cell and Molecular Biophysics at King’s College London. Following completion of her fellowship, Maddy was appointed Reader at King’s in 2013 and Professor of Cell Biology in 2015. Maddy has established collaborations with developmental biologists and clinical researchers to study adhesion receptor signalling in skin blistering, wound healing, inflammation and cancer. She works closely with physicists, biophysicists and other world-leading cell migration groups in the field to develop and apply new imaging technologies to dissect spatiotemporal cytoskeletal signalling events in live cells, tissues and whole organisms. As a result of her interest and applications of advanced microscopy, Maddy developed a strong working partnership with Nikon, which subsequently led to the establishment of the state-of-the-art, world-class Nikon Imaging Centre at King’s College London of which she is Director. Maddy also currently works alongside other biotech and pharmaceutical companies to develop and apply advanced imaging approaches to basic mechanisms that underpin drug discovery.
How chromatin organises in mammalian cells - lessons from 3D super-resolution microscopy
Dr Lothar Schermelleh
University of Oxford
2003 PhD at the Ludwig Maximilians University Munich (Advisor: Prof. Thomas Cremer)
2003-2011 Postdoctoral Researcher / Lecturer (Epigenetics & Bioimaging); Faculty of Biology, Ludwig Maximilians University Munich (Advisor: Prof. Heinrich Leonhardt).
2005-2007 Visiting Scientist with Prof. John W. Sedat, University of California, San Francisco.
Since 2011 Micron Senior Research Fellow / Principle Investigator at the Department of Biochemistry, University of Oxford
Multiphoton imaging across millimeter length scales with subcellular and subsecond resolution
Dr Spencer Smith
University of North Carolina - Chapel Hill
Spencer LaVere Smith earned is his undergraduate degree in physics and mathematics, and his Ph.D in neuroscience and neuroengineering. After postdocs at UCLA and University College London, he started his own lab at the University of North Carolina in 2011. Smith’s research uses state-of-the-art imaging, electrophysiology, and quantitative behavior to reverse engineer the neuronal activity dynamics that encode stimuli and guide behavior. His lab (slslab.org, labrigger.com) has developed novel multiphoton imaging instrumentation to measure neuronal activity across multiple brain areas simultaneously with subcellular resolution. His awards include a McKnight Technological Innovation Award (2015), a Klingenstein-Simons Fellowship (2013), and a Human Frontier Science Program Career Development Award (2012).
In vivo STED microscopy
Dr Katrin Willig
Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen
Katrin Willig studied Physics at Würzburg University and the University of New Mexico, Albuquerque, before joining Stefan W. Hell’s research team at the Max Planck Institute for Biophysical Chemistry in Göttingen in 2002. She graduated with a PhD from Heidelberg University in 2006 with a thesis on STED microscopy in the visible range. Since 2014 she has been leading her own junior research group at the Göttingen Cluster of Excellence and DFG Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB) located the Max Planck Institute of Experimental Medicine in Göttingen. She pioneered the use of fluorescent proteins for nanoscale imaging of living cells and has developed STED microscopy for imaging tissue inside living organs. She has demonstrated the strength of these technologies by in vivo imaging of the tiny protrusions (dendritic spines) on nerve cell dendrites found in the synapses inside a living mouse brain with unprecedented detail.
Admittance to this event is for registered and authorised attendees. Unfortunately we cannot permit access to visitors or allow non-registered persons to enter the meeting or exhibition areas. If you have any questions, please contact the RMS contact for this event.
Registration has now closed.
RMS Member £320
On Wednesday 27 June there will be a conference dinner at a local restaurant called the Drygate. The dinner is included in the registration fee for the Meeting.
An email will be sent to you two to three weeks before the event with final details.
Frontiers in BioImaging will take place at the Technology & Innovation Centre (TIC), University of Strathclyde, Glasgow. For travel information please refer to the webpage
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