Frontiers in BioImaging 2016
Scientific Organisers: Dr Susan Cox, Dr Ian Dobbie, Dr Maddy Parsons and Dr Theresa Ward
Registration for this event has now closed.
Frontiers in BioImaging 2016 will focus on the latest developments in applications of optical microscopy and image analysis across a range of biomedical fields. Sessions will cover both technical developments and applications of these microscopy-based approaches to key cell and molecular biology questions. The meeting will cover the key challenges in microscopy today: super-resolution imaging, phototoxicity and light sheet based methods, detection of in situ protein interactions and new tools for fluorescence visualisation and analysis.
This is an ideal meeting for both new and established researchers to engage with a broad range of imaging approaches and to make valuable contacts with leading groups in the field. There will be an exhibition held alongside this meeting, where the tea, coffee, lunch and posters will all be held. We are very grateful to the exhibitors and their support.
- 09:30 Registration, Exhibition and Coffee
- 10:30 Welcome and Introduction
SESSION ONE - IMAGING INTERACTIONS
Imaging strategies for protein interaction monitoring in live cells - Dr Simon Ameer-Beg
- 11:10 Frontiers in biochemical imaging: multiplexed FRET sensors and Optogenetics - Alessandro Esposito
- 11:25 mScarlet, a novel high quantum yield (71%) monomeric red fluorescent protein with enhanced properties for FRET- and super resolution microscopy - Prof Dorus Gadella
- 11:55 3D Visualisation of Oviductal Sperm Selection - Tania Mendonca
- 12:10 Flash Talk: Neuroscience Imaging enabled by new highly tunable and high peak power femtosecond lasers - Newport Spectraphysics
- 12:15 RMS Light Microscopy Section AGM
- 12:25 RMS Life Sciences Section AGM
- 12:35 Lunch, Exhibition and Posters
SESSION TWO - LOWER PHOTO-TOXICITY IMAGING
Light Sheet-based illumination provides the basis for highly corrected, sensor-based and fully automated microscopy - Prof Ernst Stelzer
- 14:35 Fast live-cell conventional fluorpohore nanoscopy with ImageJ through Super-Resolution Radial Fluctuations - Sîan Culley
- 14:50 Dreaming of a Digital Ocean:Integrative 3D imaging bottom up - Dr Emmanuel Reynaud
- 15:20 Stage-scanning oblique plane microscopy applied to three-dimensional time-lapse imaging of 3D cell cultures in 96-well plates - Vincent Maioli
- 15:35 Tea, Coffee, Exhibiton and Posters
SESSION THREE - SUPER-RESOLUTION
Super-resolution fluorescence imaging by dSTORM - Dr Markus Sauer
- 16:35 Determining the architecture of inactive EGF receptor oligomers on cells with 5nm resolution - Selene Roberts
- 16:50 Super-resolution imaging of the cytoskeleton - Prof Michelle Peckham
- 17:20 Confocal and super-resolution reflectance imaging of intracellular metal oxide nanoparticles - Emily Guggenheim
- 17:35 Flash Talk: Cameras for Life Science - Ruediger Bader, Hamamatsu Photonics
- 17:40 KEYNOTE SPEAKER: Imaging approaches to the immunological synapse- a key to immunotherapy - Prof Mike Dustin
- 18:30 Drinks, Buffet Dinner, Posters and Exhibition
SESSION FOUR - IMAGE ANALYSIS
Localisation Microscopy & Image Processing Challenges - Dr Bernd Rieger
- 09:30 Expanding the limits: combining submillisecond single-molecule nanoscale imaging and cellular level systems modelling - Helen Miller
- 09:45 Information in localisation microscopy - Dr Susan Cox
- 10:15 Mapping poxvirus architecture using super-resolution microscopy and single-particle analysis - Robert Gray
- 10:30 Tea, Coffee, Exhibition and Posters
SESSION FIVE - INFORMATION OVER DIFFERENT LENGTH SCALES
3D optical mesoscopy with the Mesolens - Prof Gail McConnell
- 11:30 Imaging Cancer Progression and response to Therapy at the Molecular Level in vivo - Kurt Anderson
- 11:45 Super-accurate CLEM:3D super-resolution light microscopy inside an electron microscope - Dr Lucy Collinson
- 12:15 Morphological changes in dendritic epidermal gd T cells in Hbs1l knockout mice investigated by super-resolution microscopy - Dmitry Ushakov
- 12:30 Flash Talk: Scientific Cameras and Light Sources for High End Microscope Systems - David Gibson, Photon Lines
- 12:35 Lunch, Exhibition and Posters
SESSION SIX - NEW PROBES AND LABELLING STRATEGIES
New tools to study beta-cells by CLEM - Dr Carsten Schultz
- 14:30 Split GFP - visualization of real time delivery of a functional peptide cargo into living cells - Pamela Riester
- 14:45 Engineering allosteric networks to image and control signaling in vivo - Dr Klaus Hahn
- 15:15 Single Molecule Imaging of FRET with fluorescence Anisotropy - Viviane Devauges
- 15:30 Concluding Remarks
- 16:00 Tea and Coffee
Registration for this meeting has now closed
It is expected registration on the day will open from 9.30 am on Thursday with the meeting starting at 10.30 am and the programme will finish by 5.00 pm on Friday.
Accommodation is not included in the event registration. We recommend you book your accommodation as early as possible.
On Thursday 14 July there will be a buffet dinner at the venue, this is included in the registration fee for the Meeting.
An email will be sent to you three weeks before the event with final details.
15 Hatfields, London SE1 8DJ
The nearest tube stations are:
Prof Michael Dustin
The Kennedy Institute of Rheumatology, University of Oxford
Dr Simon Ameer-Beg
King's College London
Simon Ameer-Beg is a Senior Lecturer in the Cancer Cell Biology & Imaging at King’s College, London. His research interests include: Protein-protein interactions, Multiphoton Fluorescence Lifetime Imaging, Single molecule imaging; FRET, and Fluorescence anisotropy.
Dr Lucy Collinson
EM Section Chair
The Francis Crick Institute
Lucy is Head of Electron Microscopy at The Francis Crick Institute in London. Her degree and PhD were in Microbiology, followed by a post-doctoral position in Cell Biology using light and electron microscopy to investigate membrane trafficking pathways at University College London. Following that she ran biological EM facilities, first at UCL and then at the Cancer Research UK London Research Institute, which became part of the new Francis Crick Institute in 2015. Her microscopy interests cover 3D EM, Correlative Light and EM, X-ray microscopy, image analysis, and microscope design and prototyping.
Dr Susan Cox
Light Microscopy Representative infocus Editorial Board
King's College London
Susan is a Royal Society University Research Fellow in the Randall Division of Biophysics at King's College London. Following a PhD in transmission electron microscopy at Cambridge, she spent three years at the National High Magnetic Field Laboratory at Los Alamos looking at the behaviour of the low temperature phases of strongly correlated electron systems. Her current primary interest is the development of new super-resolution localisation microscopy techniques, both through the development of optical systems and the creation of novel image analysis algorithms. She uses these techniques to investigate the behaviour of the cytoskeleton in live cells at the nanoscale. In 2015, Susan was awarded the RMS Medal for Light Microscopy and the President's Medal of the Society of Experimental Biology for the Cell Section.
Prof Dorus Gadella
University of Amsterdam
Dr Klaus Hahn
University of Carolina, USA
Dr. Hahn obtained a B.S in biochemistry from the University of Pennsylvania, followed by a doctorate in Organic Chemistry from the University of Virginia. He next focused on biosensors and microscopy as a postdoctoral fellow working with D. L. Taylor and A. Waggoner at the Center for Fluorescence Research at Carnegie Mellon University, and ultimately became an Associate Professor of Cell Biology at the Scripps Research Institute. He moved to UNC-Chapel Hill Medical School in 2004, where he is now the Thurman Distinguished Professor of Pharmacology and Director of the UNC-Olympus Imaging Center. Dr. Hahn is a recipient of an NIH Transformative Grant, the NIH’s James Shannon Director’s Award, and is a fellow of the AAAS. His lab’s work on biosensors was named one of the “10 Breakthroughs of the Decade” by Nature Reviews Molecular Cell Biology. Dr. Hahn’s lab focuses on molecular tools to visualise and control signalling in living cells, and on questions re the spatio-temporal dynamics of signalling in blood cells, currently emphasising immune receptors, platelet production, and macrophage motility. He is working on novel biosensor designs that minimally perturb signalling, and engineering allosteric networks in proteins to confer control by light or small molecules.
Prof Gail McConnell
Light Microscopy Section Deputy Chair
University of Strathclyde
Gail McConnell is Chair of Biophotonics at the Strathclyde Institute of Pharmacy and Biomedical Sciences at the University of Strathclyde. Following a first degree in Laser Physics and Optoelectronics (1998) and PhD in Physics from the University of Strathclyde (2002), she obtained a Personal Research Fellowship from the Royal Society of Edinburgh (2003) and a Research Councils UK Academic Fellowship (2005), securing a readership in 2008. Since 2004, Gail has received over £9M of research funding from a range of sources including EPSRC, MRC, BBSRC, EU and industry. The work in Gail’s group involves the design, development and application of linear and nonlinear optical instrumentation for biomedical imaging, from the nanoscale to the whole organism. She is a Fellow of the Institute of Physics, and a Fellow of the Royal Microscopical Society
Prof Michelle Peckham
University of Leeds
Michelle Peckham is Professor of Cell Biology in the Faculty of Biological Sciences. She obtained a BA in Physiology of Organisms at the University of York, and a PhD in Physiology at University College London. She moved to King's College London, and started to use a specialised form of light microscopy (birefringence) to investigate muscle crossbridge orientation. She then worked at UCSF, San Francisco for a year, where she used fluorescence polarisation to investigate muscle crossbridges. She moved back to the UK, to the University of York, to work on insect flight muscle. In 1990 she was awarded a Royal Society University Research fellowship, based at King's College London, and began working on the cell and molecular biology of muscle development, and started to use live cell imaging to investigate muscle cell behaviour in cultured cells, and confocal microscopy to investigate their cytoskeleton. She collaborated with Graham Dunn to use Digitally Recorded Interference Microscopy with Automatic Phase Shifting (DRIMAPS) to investigate cell crawling behaviour. She moved to Leeds in 1997 as a Lecturer, and has continued to use a wide range of both light and electron microscopy approaches to investigate the molecular motors and the cytoskeleton.
Dr Emmanuel Reynaud
University College Dublin
Dr Emmanuel G. Reynaud is a Lecturer in Cell Biology at University College Dublin. After a PhD in Life Sciences from the University of Paris XI/Orsay, he received an EMBO Long Term Fellowship and moved to the European Molecular Biology Laboratory in Heidelberg Germany where he developed new methods in Cell Biology including laser nanosurgery approaches to study the Golgi biogenesis. He was later involved in the development of the Light Sheet based Fluorescence Microscopy as a member of the Light Microscopy Group headed by Ernst H.K. Stelzer. His laboratory is combining an R&D prototyping space and a cell biology laboratory to investigate the functions of epithelia in healh and diseases using a wide range of model systems from coral reefs to single vesicle. In 2014, he was awarded the Chevalier (Knight) of the Ordre des Palmes Académiques, one of the highest civilian honours bestowed on academics and educators by the French state.
Dr Bernd Rieger
Delft University of Technology
Dr Markus Sauer
University of Würzburg
Markus Sauer studied Chemistry at the University Heidelberg where he received his Diploma in 1991. He finished his PhD 1995 in Physical Chemistry under the supervision of Prof. Dr. Jürgen Wolfrum. 1998 he has been awarded the BioFuture Prize for Detection, Analysis and Handling of Single Molecules, which allowed him to establish his own group for single-molecule fluorescence detection and single-molecule DNA sequencing. Since 2009 he is Professor and Chair of the Department of Biotechnology and Biophysics at the Julius Maximilian University Würzburg. His research interests are single-molecule fluorescence spectroscopy and imaging with a particular focus on super-resolution fluorescence imaging by direct stochastic optical reconstruction microscopy (dSTORM) and its applications in neurobiology and immunology. He has published more than 250 journal papers and coordinates several national super-resolution microscopy projects.
Dr Carsten Schultz
Cell Biology & Biophysics Unit
Prof Ernst Stelzer
Goethe University of Frankfurt
The RMS would like to thank all of the below sponsors of the Frontiers in BioImaging meeting. There will be an exhibtion during the meeting giving delegates a great opportuntity to network along side tea/coffee and lunch.
Exhibtion space is now full. There are still many ways of getting involved through sponsorship opportunities, such as; advertising, promotonal items in the delegate bags or dinner sponsorship. Please contact Chloe Goode for more details.
Andor Technology plc is a global leader in the pioneering and manufacturing high performance scientific imaging cameras, spectroscopy solutions and microscopy systems for research and OEM markets. Andor has been innovating the photonics industry for over 20 years and aims to continue to set the standard for high performance light measuring solutions that allow consumers to perform light measurements previously considered impossible. Through continuous dialogue with customers and strong teamwork, Andor continues to innovate ground-breaking products that improve the world in which we live.
Find out more about BioLegend here
Please find more information on Bitplane here
Cairn is an Independent research-orientated company specialising in microscopy solutions for the life sciences. From our origins as a provider of turnkey optical systems for fast intracellular ion measurements we have maintained close links with our academic customer base enabling us to develop a broad range of products and solutions to meet their expanding needs. Our strength lies in our understanding of both the requirements of the research microscopy community and of the advances in technology from which they can benefit. We have a “can do”, informal, and friendly attitude and are always willing to give advice. The company has evolved organically to encompass four distinct, but interrelated areas.
We are instrument designers, manufacturers and vendors of both Illumination and Detection products. Our illumination products encompass a wide range of light sources, adapters and specialist launch systems; typically used in conjunction with a third-party, or Cairn-built, microscope or macrosope. On the detection side our primary focus is on multi-channel imaging using one or more third-party cameras. These products are largely based around optical techniques, most importantly, fluorescence, optogenetics, photolysis and transmitted light imaging. Although fully independent we are closely allied with Chroma Technology and a large number of our illumination and detection products make use of their high performance interference filters.
Carl Zeiss is an innovative technology leader in the fields of optics, precision engineering and electronic visualisation. Time and time again, we set new, pioneering standards in sophisticated technology for recognising, experiencing, measuring, analysing, structuring and processing a wide spectrum of objects. With professional optics we meet the expectations of even our most critical customers - not only in the fields of research, medicine, industry, but also for use in leisure activities.
Hamamatsu Photonics is a world-leading manufacturer of opto-electronic components and systems and employs over 3000 staff worldwide. The corporate headquarters are based in Hamamatsu City, Japan along with various manufacturing plants and central research laboratories. Since its inception in 1953, Hamamatsu Photonics has expanded to now enjoy a global presence throughout Asia, Europe and North America.
Hamamatsu Photonics’ corporate philosophy stresses the advancement of Photonics through extensive research and development. Hundreds of new opto-electronic products are introduced to the market each year and many Hamamatsu products are regarded as state-of-the-art. Hamamatsu sources, detectors and imaging products are designed to cover the entire optical spectrum, from nuclear radiation, x-ray, Ultraviolet (UV), Visible and Infrared radiation. Hamamatsu devices provide solutions for a wide variety of applications including analytical, industrial and medical instrumentation.
Find out more about Laser Lines here
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LaVision BioTec GmbH
LaVision BioTec supplies advanced microscopy solutions for life sciences. UltraMicroscope II utilizes six light sheets to excite large samples with fluorescence light. Moving the sample through the light sheets generates 3D image stacks at cellular resolution. TriM Scope II is a modular multi-photon microscopy platform combining single- and multi-beam operation for deep in-vivo imaging. Multiple accessories provide customization.
Leica UK Ltd
Leica Microsystems is a leading manufacturer and supplier of high precision optical solutions based on microscopes and related instruments. The company manufactures a comprehensive portfolio of products used in a wide variety of areas requiring vision, measurement and analysis, including applications in the life sciences (such as bio-technology research and medicine) and the material sciences.
Linkam Scientific Instruments
Linkam develops and manufactures a broad range of heating and freezing stages for both OEM and end users to visualize and explore materials properties. Used in conjunction with light microscopes and other forms of spectroscopy, Linkam stages are found in thousands of laboratories worldwide with the most successful microscope heating stage, the THMS600, selling over 4,000 units alone. Linkam is the market leader in temperature controlled microscopy.
LOT Quantum Design
Find out more about LOT Quantum Design here
Mad City Labs
Find out more about Mad City Labs here
Find more information on PCO here
Founded in 1978, Photometrics is the world’s premier designer and manufacturer of high-performance CCD and EMCCD cameras for the life sciences.
The original architect of the world’s 1st scientific grade microscopy EMCCD camera, researchers across the globe rely on Photometrics’ state-of-the-art imaging instrumentation, including its popular CoolSNAP™, Cascade®, QuantEM® and Evolvecameras to meet their most demanding application requirements.
Photon Lines supplies a range of scientific cameras (including CCD cameras, CMOS and sCMOS), lasers and laser components which are optimised for Lightsheet microscopy. We also supply advanced scientific imaging solutions primarily into the area of biophotonics, such as Label-Free Live Cell Imaging Systems and fluorescence microscopy equipment including the products of PhaseView, who have engineered unique 3D imaging technologies for life science microscopy applications. Based on a novel remote focusing principle, PhaseView imaging products allow for high speed 3D imaging which is only limited by camera frame rate, while keeping bio-specimens in a stable position without moving the objective or stage. Using this acquisition principle, PhaseView’s Alpha3 light sheet fluorescence microscope addresses the issue of high temporal resolution along with spatial high-resolution, to achieve 3D imaging of dynamic biological processes.
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