Super-resolution Workshop 2017
Designed to talk about the current challenges in developing and using super-resolution microscopy with lots of time for discussion, this year the workshop will focus on the topic of labelling. Thoughts and ideas are encouraged to help define what is good/best practice around these challenging techniques, and give an insight into future potential developments.
Registration will close on Friday 23 June.
- 08:30 Registration
- 09:00 DNA Paint - Ralf Jungmann, Max Planck, Munich
- 09:30 Developing sample preparation, labelling and imaging protocols for using Affimers in dSTORM - Ruth Hughes, University of Leeds
- 09:50 Multimodal super-resolution imaging of chromatin organisation - Lothar Schermelleh, University of Oxford
- 10:20 Coffee Break
- 10:45 Iterative expansion microscopy - Jae-Byum Chang, MIT, USA
- 11:15 Improved 3D localization with supercritical angle fluorescence emission - Sandrine Leveque-Fort, CNRS Paris Sud
- 11:45 Argolight Techno Bite
- 11:50 Cairn Research Ltd Techno Bite
- 11:55 Nikon Techno Bite
- 12:00 Lunch
- 13:00 Labeling and quantitative imaging of complex biological samples for super-resolution microscopy - Christian Soeller, University of Exeter
- 13:30 Nanoscale organisation of cell surface receptors - Caron Jacobs, UCL
- 13:50 Investigating bacterial cell division using Super-resolution Microscopy - Victoria Lund, University of Sheffield
- 14:10 Oxford Nanoimaging Techno Bite
- 14:15 Photometrics and Qimaging Techno Bite
- 14:20 Coffee Break
- 14:40 Extracting and analysing localisation data, using palm3d - Alistair Curd, University of Leeds
- 15:00 When super-resolution meets model-based analysis - Romain Laine, University of Cambridge
- 15:20 NanoJ-SQUIRREL - is your image nuts? - Sian Culley, UCL
- 15:40 Localisation microscopy – should seeing be believing? - Susan Cox, King's College London
- 16:10 Wrap up and final discussion
- 16:30 End of Workshop
Prof Michelle Peckham
Executive Honorary Secretary
University of Leeds
Michelle Peckham is Professor of Cell Biology in the Faculty of Biological Sciences. She obtained a BA in Physiology of Organisms at the University of York, and a PhD in Physiology at University College London. She moved to King's College London, and started to use a specialised form of light microscopy (birefringence) to investigate muscle crossbridge orientation. She then worked at UCSF, San Francisco for a year, where she used fluorescence polarisation to investigate muscle crossbridges. She moved back to the UK, to the University of York, to work on insect flight muscle. In 1990 she was awarded a Royal Society University Research fellowship, based at King's College London, and began working on the cell and molecular biology of muscle development, and started to use live cell imaging to investigate muscle cell behaviour in cultured cells, and confocal microscopy to investigate their cytoskeleton. She collaborated with Graham Dunn to use Digitally Recorded Interference Microscopy with Automatic Phase Shifting (DRIMAPS) to investigate cell crawling behaviour. She moved to Leeds in 1997 as a Lecturer, and has continued to use a wide range of both light and electron microscopy approaches to investigate the molecular motors and the cytoskeleton.
Dr Susan Cox
Light Microscopy Representative infocus Editorial Board
King's College London
Susan is a Royal Society University Research Fellow in the Randall Division of Biophysics at King's College London. Following a PhD in transmission electron microscopy at Cambridge, she spent three years at the National High Magnetic Field Laboratory at Los Alamos looking at the behaviour of the low temperature phases of strongly correlated electron systems. Her current primary interest is the development of new super-resolution localisation microscopy techniques, both through the development of optical systems and the creation of novel image analysis algorithms. She uses these techniques to investigate the behaviour of the cytoskeleton in live cells at the nanoscale. In 2015, Susan was awarded the RMS Medal for Light Microscopy and the President's Medal of the Society of Experimental Biology for the Cell Section.
Dr Jae-Byum Chang
Dr Sian Culley
Siân Culley is a postdoc in the Quantitative Imaging and Nanobiophysics group at the MRC Laboratory for Molecular Cell Biology at UCL. After doing an MSci project with Prof. Jonathan Ashmore in two-photon imaging of calcium signalling in inner hair cells, she moved into the field of super-resolution microscopy for her PhD with Dr Angus Bain investigating photophysical processes in CW-STED microscopy. In 2014 she joined Ricardo Henriques’ group, and her current research interests lie in developing open source hardware and analytics for live cell super-resolution microscopy. She also has an active interest in promoting women in microscopy.
Dr Alistair Curd
University of Leeds
Dr Ruth Hughes
University of Leeds
Prof Ralf Jungmann
Max Planck Institute of Biochemistry
Ralf Jungmann studied Physics at Saarland University, followed by a one-year diploma research stay with Paul Hansma at UC Santa Barbara where he worked on functional imaging of bone ultrastructure using Atomic Force Microscopy and High-Speed-Photography.
In 2007, Jungmann joined Prof. Friedrich C. Simmel’s lab at TU München as a Ph.D. student and was among the first researchers in Germany to apply and extend the DNA origami technique. During his Ph.D., Jungmann applied single-molecule fluorescence techniques to DNA Nanotechnology, constructing the first nanoscopic DNA origami “rulers” for super-resolution microscopy. He also pioneered a novel type of super-resolution microscopy, termed DNA-PAINT, that uses programmable DNA molecules as imaging probes.
After receiving his Ph.D. at TUM, he moved to the labs of Prof. Peng Yin and Prof. William M. Shih at the Wyss Institute for Biologically Inspired Engineering at Harvard University as an Alexander von Humboldt fellow. At Harvard, Jungmann worked on applications of DNA-PAINT for multiplexed cellular imaging.
In 2014, Jungmann received an Emmy Noether Fellowship from the German Research Foundation (DFG) and since then heads the research group “Molecular Imaging and Bionanotechnology” at the MPI of Biochemistry and the LMU Munich. In 2015, Jungmann co-founded Ultivue, a US-based company commercializing imaging reagents for DNA-PAINT super-resolution microscopy.
In 2016 he received an ERC Starting Grant to bring DNA-based super-resolution imaging from single molecules to whole cells and tissues. Since August 2016, he is Professor of Physics at the LMU Munich.
Dr Romain Laine
University of Cambridge
Romain studied Natural Sciences in at the ESPCI, Paris before moving to Imperial College London for a MSci in Optics and Photonics. He then carried on with an MRes in Chemical biology of health and disease and PhD at the Photonics group of Imperial specialising in advanced FLIM microscopy applied to cellular biology, with Prof. Paul French. He then moved to Cambridge to join Prof. Clemens Kaminski at the Laser Analytics Group and studies virus ultra-structure by applying state-of-the-art super-resolution microscopy and analytical methods.
Institut des Sciences Moléculaires d'Orsay
Sandrine Lévêque-Fort is a CNRS Researcher Director at the Institute of molecular science in Orsay (ISMO). She obtained her PhD on the development of a new acousto-optic imaging approach for imaging through scattering media in the Optical Lab of ESPCI. She then became a postdoctoral fellow in the physics department of Imperial College, where she started to develop time resolved fluorescence microscopy. She joined the CRNS in 2001, when she started to develop different strategies to improve fluorescence microscopy such as plasmonics. Since 2009, her research focus on supercritical angle fluorescence microscopy and super-resolution microscopy techniques.
Dr Victoria Lund
University of Sheffield
Dr Lothar Schermelleh
University of Oxford
2003 PhD at the Ludwig Maximilians University Munich (Advisor: Prof. Thomas Cremer)
2003-2011 Postdoctoral Researcher / Lecturer (Epigenetics & Bioimaging); Faculty of Biology, Ludwig Maximilians University Munich (Advisor: Prof. Heinrich Leonhardt).
2005-2007 Visiting Scientist with Prof. John W. Sedat, University of California, San Francisco.
Since 2011 Micron Senior Research Fellow / Principle Investigator at the Department of Biochemistry, University of Oxford
Prof Christian Soeller
University of Exeter
Prof. Christian Soeller obtained his PhD at the Max Planck Institute for Fluid Mechanics in Goettingen. He conducted postdoctoral research at the University of London where he developed and applied multi-photon microscopy to study the cell biology of cardiac contraction. He then took up a faculty position in the School of Medicine at the University of Auckland, New Zealand, where he continued his use of advanced microscopy techniques. As early adopters of super-resolution microscopy his group was able to see arrays of receptors in muscle cells with near molecular resolution for the first time. In 2012 he moved to the University of Exeter, UK, where he is a Professor of Physical Cell Biology in the interdisciplinary Living Systems Institute and continues his research into the use and advancement of super-resolution approaches to enable new biological insight.
Cairn is an Independent research-orientated company specialising in microscopy solutions for the life sciences. From our origins as a provider of turnkey optical systems for fast intracellular ion measurements we have maintained close links with our academic customer base enabling us to develop a broad range of products and solutions to meet their expanding needs. Our strength lies in our understanding of both the requirements of the research microscopy community and of the advances in technology from which they can benefit. We have a “can do”, informal, and friendly attitude and are always willing to give advice. The company has evolved organically to encompass four distinct, but interrelated areas.
We are instrument designers, manufacturers and vendors of both Illumination and Detection products. Our illumination products encompass a wide range of light sources, adapters and specialist launch systems; typically used in conjunction with a third-party, or Cairn-built, microscope or macrosope. On the detection side our primary focus is on multi-channel imaging using one or more third-party cameras. These products are largely based around optical techniques, most importantly, fluorescence, optogenetics, photolysis and transmitted light imaging. Although fully independent we are closely allied with Chroma Technology and a large number of our illumination and detection products make use of their high performance interference filters.
To find out more about GATTAquant GmbH click here.
Institute of Physics Biological Physics Group
Nikon UK Ltd
Nikon Instruments is a leading manufacturer in light microscopy and metrology solutions. With over 100 years’ experience in optical design and a strong core technology group, Nikon has become a truly iconic brand for all types of imaging.
Nikon’s philosophy is to meet needs and exceed expectations. We specialise in the development of optical products, building an unbeatable reputation for lens technology and precision optics. We pride ourselves on providing high contrast, high definition, and aberration free images.
Oxford NanoImaging Limited
Oxford Nanoimaging Limited is a company originating in the Clarendon Laboratory, Department of Physics at the University of Oxford. Professor Achillefs Kapanidis and PhD student, Bo Jing, lead a collaborative, inter-disciplinary team that has pioneered innovative technologies to produce an elegant benchtop super-resolution microscope. The Nanoimager has a footprint of just 21 cm x 21 cm yet packs the capability of a much larger, conventional microscopy platform delivering super-resolution and single-molecule performance. With a significantly lower cost of entry, researchers will now be able to obtain benchtop nanoscale imaging at a fraction of the price of earlier systems without the need for a large laboratory and skilled operators. As Professor Kapanidis says, “I wish I had this when I was a graduate student.”
Founded in 1978, Photometrics is the world’s premier designer and manufacturer of high-performance CCD and EMCCD cameras for the life sciences.
The original architect of the world’s 1st scientific grade microscopy EMCCD camera, researchers across the globe rely on Photometrics’ state-of-the-art imaging instrumentation, including its popular CoolSNAP™, Cascade®, QuantEM® and Evolvecameras to meet their most demanding application requirements.
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