Imaging ONEWORLD covers topics including sample preparation, labelling strategies, experimental workflows, and ‘how-to’ image and analyse. The webinars examine inspiring new scientific ideas and facilitate collaborations, with speakers also available for questions and answers. The organisers, core facility staff from the University of Cambridge, Gurdon Institute, MRC-LMB and the ICR/Royal Marsden Trust are also able to continue the discussion and provide advice on your imaging projects.
If you would like to speak at a future meeting in this series or have a suggestion for a speaker, please fill in the suggestion form. For any questions in regards to the Imaging ONEWORLD series, please contact Jessica Cole.
Scientific Organisers
Alex Sossick
Alex heads the Imaging Facility at the Gurdon Institute, which includes a variety of microscopy techniques including confocal, high throughput and deconvolution. He is keen to raise the level of microscopy understanding and application, and runs and takes part in various microscopy courses.
Stefanie Reichelt
Stefanie Reichelt, PhD has been head of the light microscopy facility at the CRUK Cambridge Institute. The core provides state-of-the-art imaging resources, training courses for scientists and students and develop new imaging systems as well as user-friendly analysis and acquisition tools for specific research applications. Stefanie is now Public Engagement Manager for the Biomedical Schools and teaches academically at Cambridge University, in scientific workshops and out-reach events. (http://cargocollective.com/StefanieReichelt)
- Email:
- [email protected]
Alessandro Esposito
Dr Alessandro Esposito obtained a PhD in Biophysics in 2006 working at the University of Utrecht and the European Neuroscience Institute in Goettingen for which he was awarded the ‘Sergio Ciani’ award by the Italian Society of Pure and Applied Biophysics. At the University of Cambridge, he then developed novel analytical tools contributing to redefining models of red blood cells homeostasis infected by P. falciparum (malaria). In recognition of his early work, in 2009 Alessandro was awarded a Life Science Interface fellowship by the EPSRC to establish foster the development of heavily multiplexed biochemical imaging. Soon after he moved to the MRC Cancer Unit where he lead the ‘Systems Microscopy initiative’ and retrained in cancer biology. During these years, Alessandro’s work developed into two research streams: i) the study of cellular responses to DNA damage and mutations in signalling pathways and ii) the innovation of biochemical imaging technologies. His team contributed to revealing the vast cell-to-cell variability in stress responses of genetically identical cells, a feature of biological systems that hinder the efficacy of disease management and therapeutic efficacy. Since 2019, Alessandro leads a transdisciplinary research programme at the MRC Cancer Unit in Cambridge devoted to understanding how DNA damage and mutations in KRAS derange homeostatic programmes leading to cancer. His group combines multi-omics data with single-cell biochemical imaging techniques aiming to achieve a deeper understanding of cancer phenotypes during the earliest stages of carcinogenesis, with particular attention to cell-to-cell variability of non-genetic origin and cell-to-cell communication.
Kirti Prakash
Kirti Prakash is a computer scientist by training (Bachelors and Masters degree) but a biologist at heart (PhD degree). Kirti aspires to be an inventor and develop new imaging tools for cell biology and neuroscience. Kirti did his Masters in Computer Science from Aalto University (Finland) and PhD in Biology from Heidelberg University (Germany). During his PhD, he developed a new method to image DNA which led to the first high-resolution images of the epigenetic landscape of meiotic chromosomes and mechanisms behind chromosome condensation. The doctoral research earned him several awards including Springer Best PhD Thesis Prize. After his PhD, he did a couple of postdocs at Carnegie Institution for Science (USA) and University of Cambridge (UK). The primary highlights of his research here were laser-free superresolution microscopy and development of a high-content imaging pipeline to quantify single-cell gene expression. Formerly at the National Physical Laboratory (NPL), and currently working at the Institute for Cancer Research (ICR) and Royal Marsden Trust, he is working on microscope development and image analysis.
Series Schedule
You can register for an individual meeting by clicking on the corresponding link below.
If you would like to sign up for the whole series, please register for the next occurence and select the 'Enrol for Whole Series' box during registration.
Upcoming Lectures
Please note, all meetings begin at 13:00 UK time.
18 July 2022 - Anna Kreshuk, Group Leader at EMBL Heidelberg, Germany - Lecture TBA
1 August - 5 September 2022 - No Imaging ONEWORLD - Summer break
12 September 2022 - 'Designing a Rigorous Quantitative Imaging Experiment: Image Correction and Validation' by Jennifer Waters, Director of the Nikon Imaging Center, Harvard Medical School, MA, USA
19 September 2022 - Kristina Ganzinger from AMOLF, Netherlands - Lecture TBA
26 September 2022 - 'Real Time Widefield TCSPC based Fluorescence Lifetime Imaging' by Dr Graham Hungerford from Horiba Scientific, UK
Previous Lectures
Many for the Imaging ONEWORLD lectures have been recorded and uploaded to our YouTube channel.
From all corona-born seminar series I've attended, together with the Neubias Academy I enjoy this here the most! Thank you so much to all the organizers and speakers.
2022 Lectures
20 June 2022 - 'Data fusion in localization based super-resolution microscopy' by Professor Dr Bernd Rieger, Department of Imaging Physics at the Faculty of Applied Sciences, Delft University of Technology, The Netherlands. Lecture available on YouTube
13 June 2022 - '3D Imaging at different scales using single-objective light-sheet microscopy' by Dr. Jean-Baptiste Sibarita, Group Leader at the Centre national de la recherche scientifique, France. Lecture available on YouTube
6 June 2022 - 'Correlative Light and Electron Microscopy (CLEM): Combining Ultrastructural and Molecular Imaging' by Jesse Aaron, Applications Scientist from the Advanced Imaging Center, HHMI Janelia Research Campus, VA, United States. Lecture available of YouTube
30 May 2022 - 'Overcoming physical resolution limits of fluorescence microscopes with sparse deconvolution' by Professor Liangyi Chen from the Peking University in Beijing, China. Lecture available on YouTube
23 May 2022 - 'Imaging how bacteria sense and repair DNA damage at the single-molecule level' by Professor Stephan Uphoff from the Department of Biochemistry at Oxford University, UK. Lecture available on YouTube
9 May 2022 - 'Super-resolution microscopy in biomedical research: Challenges and potentials' By Dr Christian Eggeling from the University of Göttingen, Germany. Lecture available on YouTube
25 April 2022 - 'Quantitative imaging of the spatio-angular architecture without label' by Dr. Shalin Mehta from Chan Zuckerberg Biohub in San Francisco. Lecture available on YouTube
4 April 2022 - 'Molecular memories in C. elegans nematodes' by Professor Oded Rechavi from Tel Aviv University, Israel. Lecture not available.
28 March 2022 - 'Looking ever deeper – an introduction to three-photon microscopy' by Dr Christian Wilms from Scientifica Ltd, UK. Lecture available on YouTube
21 March 2022 - 'Correlative super-resolution microscopy technique to probe living Neuronal synapses' by Dr V. V. G. Krishna Inavalli from the Center for Cancer Immunology (CCI), University of Southampton, UK. Lecture available on YouTube
28 February 2022 - 'Microscopy reproducibility for the real world: building interactive tools to document light microscopy experiments based on community specifications' by Dr. Caterina Strambio-De-Castillia from the University of Massachusetts Medical School, MA, USA. Lecture available on YouTube
21 February 2022 - 'Imaging cytoskeletal dynamics in invading cancer cells' by Professor Maddy Parsons from the King's College London, UK. Lecture available on YouTube
14 February 2022 - 'Super-resolution expansion microscopy' by Professor Markus Sauer from the University of Würzburg, Germany. Lecture available on YouTube
7 February 2022 - 'Metal and grahene induced energy transfer imaging' by Professor Jörg Enderlain from the Georg August University, Germany. Lecture available on YouTube
31 January 2022 - 'Pictures at an exhibition: why and how you should publish your image data' - Dr Matthew Hartley of EMBL-EBL, UK. Lecture available on YouTube
2021 Lectures
13 December 2021 - 'Smart Microscopy for Everyone: the OpenFlexure Microscope' - Dr Richard Bowman of the University of Bath. Lecture available on YouTube
29 November 2021 - Illuminating the Biochemical Activity Architecture of the Cell - Dr Jin Zhang, UC San Diego
22 November 2021 - 'Organization of translating secretome mRNAs on endoplasmic reticulum' - Dr Heejun Choi, Janelia Research Campus. Lecture available on YouTube
15 November 2021 - 'From images to information: enhancing resolution and improving accuracy' - Dr Susan Cox, King's College London. Lecture available on YouTube
8 November 2021 - 'Quantitative 4D microscopy gives unique insights into immune cell function, cellular infection and cell death mechanisms' - Dr Kelly Rogers, Walter and Eliza Hall Institute of Medical Research. Lecture available on YouTube
1st November 2021 - 'Modelling and determining 3D supramolecular structure from single-molecule localisation data, despite many missing localisations.' - Dr Alistair Curd. Lecture available on YouTube
25 October 2021 - 'Publishing methodological developments in imaging and microscopy' - Rita Strack, Nature Methods
18 October 2021 - 'Motion of single molecular tethers reveals dynamic subdomains at organelle contact sites' - Dr Chris Obara, Janelia Research Campus. Lecture available on YouTube
11 October 2021 - 'Finding the needle in the haystack with 3D correlative light and electron microscopy' - Dr Lucy Collinson, Francis Crick Institute. Lecture available on YouTube
4 October 2021 - 'Using Affimers and finding patterns in super-resolution images' - Professor Michelle Peckam, Leeds University. Lecture available on YouTube
27 September 2021 - 'How do human cells divide? Developing microscopy methods to extend the frontiers of imaging photosensitive samples.' - Professor Viji Mythily Draviam, Queen Mary, University of London
20 September 2021 - New single molecule methods to study protein dynamics on membranes - Professor Petra Schwille, Max Planck Institute of Biochemistry. Lecture available on YouTube
6 September 2021 - "Exploring molecular organization and orientation in living cells using fluorescence anisotropy imaging" - Professor Jitu Mayor, National Centre for Biological Sciences
13 September 2021 - "High-speed 3D imaging with Multifocus Microscopy" - Asst Professor Sara Abrahamsson and PhD Candidate Eduardo Hirata, Baskin School of Engineering, University of California
26 July 2021 - Quantification of cell shape and intracellular flows based on DIC object detection - Dr Chaitanya Athale, Indian Institute of Science Education and Research
19 July 2021 - Deep Learning in Microscopy - Professor Udo Birk, Institute for Photonics and ICT
12 July 2021 - Cryo Super-Resolution Fluorescence Microscopy for Correlation with Cryo-EM - Professor Rainer Kauffman, University of Hamburg
28 June 2021 - Enhanced FIB-SEM: Large Volume Whole Cells and Tissues Imaging at Fine Resolutions - Dr Shan Xu, Janelia Research Campus
21 June 2021 - Discerning how actin regulatory proteins give rise to filopodia using multi-channel timelapse imaging and quantification - Dr Jenny Gallop, Cambridge University
14 June 2021 - Adaptive optics for microscopy: the past, the present and the future - Professor Martin Booth, University of Oxford. Lecture available on YouTube
7 June 2021 - 'Horses for the courses: strategic adaptation of super-resolution microscopy modalities to solve the structural basis of cell signalling' - Dr Izzy Jayasinghe, The University of Sheffield
24 May 2021 - 'Spatially Modulated Illumination Microscopy: Application Perspectives in Nuclear Nanostructure Analysis' - Professor Christoph Cremer, Ruprecht-Karls-University, Heidelberg
17 May 2021 - 'Novel Molecular and Computational Tools for Studying Organelle Dynamics' - Uri Manor, Salk Institute for Biological Studies'
10 May 2021 - 'Using the Mesolens for optical mesoscopic imaging with sub-cellular resolution' - Professor Gail McConnell, University of Strathclyde
26 April 2021 - 'Automatic whole cell organelle segmentation in volumetric electron microscopy' - Aubrey Weigel, Janelia Research Campus
19 April 2021 - 'How do you build a wall? Using and developing advanced live cell fluorescence microscopy to understand how bacteria divide' - Seamus Holden, Newcastle University
12 April 2021 - 'Emerging imaging technologies to study cell architecture, dynamics and function' - Jennifer Lippincott-Schwartz, Janelia Research Campus
22 March 2021 - "Gaia: ESA's Two Billion Star Map of the Milky Way" - Nicholas Walton, University of Cambridge
15 March 2021 - '3D Imaging of Cells by FIBSEM with Correlation to Cryo Fluorescence Microscopy' - Harald Hess, Janelia Research Campus
8 March 2021 - 'Protein-protein interactions at the cellular interface: Biophotonics approaches to quantitative FRET measurements' - Simon Ameer-Beg, King's College London
1 March 2021 - 'Biological Discovery from Large-Scale Imaging Data' Heba Sailem, University of Oxford
22 February 2021 - 'GPU-accelerated 3D image processing for everyone' - Robert Haase, Technische Universitat Dresden
15 February 2021: 'Primed Conversion: The New Kid on the Block for Photoconversion' - Periklis Pantazis, Imperial College London
8 February 2021: 'DNA Based, Monofunctionalized quantum dots for single particle BioImaging of proteins in living cells' - Dhiraj Devidas Bhatia, Indian Institute of Technology
1 February 2021: 'The Power in your Pocket - Your Smartphone as a Super-Resolution Microscope' - Benedict Diederich, Leibniz Institute for Photonic Technology
25 January 2021: "Deep learning-based point scanning super resolution imaging" - Linjing Fang, Salk Institute for Biological Sciences
11 January 2021: 'Monitoring CRISPR-Cas target search in live bacteria using single-molecule localisation microscopy' - Johannes Holbein, Wageningen U&R
2020 Lectures
14 December 2020: 'A deep learning strategy to segment nuclei with only 3 annotated images' - Thierry Pecot, Medical University of South Carolina. Lecture available on YouTube
7 December 2020: "Decrypting MINFLUX and laser-free super-resolution microscopy" - Kirti Prakash, National Physical Laboratory. Lecture available on YouTube
30 November 2020: "Biological imaging using mass spectrometry – new concepts for a National Capability at the Rosalind Franklin Institute" - Ian Gilmore, National Physical Laboratory. Lecture available on YouTube
23 November 2020: "Chemical Microscopy - Making the Invisible Visible and Vice Versa" Sapun Parekh, The University of Texas at Austin. Lecture available on YouTube
9 November 2020: 'Oblique Plane Microscopy' - Chris Dunsby, Imperial College, London, UK. Lecture available on YouTube
2 November 2020: 'Unfinished tales: three short stories interrupted by the pandemic' - Hari Shroff, Laboratory of High Resolution Optical Imaging (HROI), NIBIB, Bethesda MD, USA
26 October 2020: 'High-Resolution, Large Field-of-View, and Multi-View Single Objective Light-Sheet Microscopy' - Loic Royer, Chan Zuckerberg Biohub, San Francisco, CA, USA
19 October 2020: 'Direct Optical Nanoscopy with Axially Localized Detection' - Sandrine Lévêque-Fort, CNRS/ Univ. Paris Sud, France
12 October 2020: 'Metabolic Index” measurements using the Phasor/FLIM method' - Michelle Digman, Department of Biomedical Engineering 3103 Natural Sciences II Irvine, CA
5 October 2020: "Novel nanoscopes (MoNaLISA, Smart RESOLFT, Adaptive STED) with the use of new molecular switchers (rsFusionRed)' - Ilaria Testa, KTH Royal Institute of Technology, STOCKHOLM, Sweden
28 September 2020: 'High-speed bio-imaging: Visualizing fast dynamics in biology and neuroscience' - Robert Prevedel, EMBL. Heidelberg
21 September 2020: 'Mapping microscopic viscosity and temperature using molecular rotors and FLIM' - Marina Kuimova, Imperial College, London
14 September 2020: 'How thin is your light sheet? Tips to survive the data apocalypse.' Emmanuel G. Reynaud, University College Dublin, Republic of Ireland
7 September 2020: 'Superresolution microscopy for structural cell biology' - Jonas Ries, EMBL, Heidelberg, Germany
27 July 2020: 'How to stay focused in 3D microscopy' - Reto Fiolka, UT Southwestern, Dallas TX, USA. Lecture available on University of Cambridge Streaming Media Service
20 July 2020: 'Tissue-Scale Cell Biology with Axially Swept Light-Sheet Microscopy' - Kevin Dean, Assistant Professor, and Director of the Microscopy Innovation Lab, UT Southwestern Medical Center, Dallas, TX, USA. Lecture available on University of Cambridge Streaming Media Service
13 July 2020: "Computational Methods for Visualization, Simulation, and Restoration Microscopy Data' - Martin Weigert, EPFL, Lausanne, Switzerland. Lecture available on University of Cambridge Streaming Media Service
6 July 2020: 'Dynamic integration of metabolism and growth factor signaling in single cells' - John Albeck, UC Davis, Davis, USA. Lecture available on University of Cambridge Streaming Media Service
29 June 2020: 'Generic Deconvolution' Rainer Heintzmann, IPC, Friedrich-Schiller-Universität & Leibniz Institute of Photonic Technology, Jena, Germany. Lecture available on University of Cambridge Streaming Media Service
22 June 2020: 'Towards Decentralised Life-Science Imaging Data Resources - Large Scale Data Ecosystem for Single Cell Imaging.' - Aleksandra Tarkowska, Wellcome Sanger Institute, Cambridge, UK. Lecture available on University of Cambridge Streaming Media Service
15 June 2020: 'Expansion Microscopy' - Ed Boyden, MIT Medialab & McGovern Institute, Cambridge, MA, USA. Lecture available on University of Cambridge Streaming Media Service
8 June 2020: 'Imaging Brain Organoids' - Madeline Lancaster, MRC LMB, Cambridge, UK. Lecture available on University of Cambridge Streaming Media Service
1 June 2020: '3D super-resolution microscopy of chromatin ‘blobs’' - Lothar Schermelleh, MICRON, Oxford, UK. Lecture available on University of Cambridge Streaming Media Service
18 May 2020: 'Imaging and analysing large, living samples with light-sheet microscopy' - Kate McDole, MRC-LMB, Cambridge, UK
11 May 2020: 'Probing and controlling cell biochemistry by light: FRET/FLIM & Optogenetics' - Alessandro Esposito, MRC Cancer Unit, Cambridge, UK