Scientific Organisers: Stefanie Reichelt, Alex Sossick, Nick Barry, Alessandro Esposito and Kirti Prakash

The meeting will begin at 13:00GMT.

As part of the 'Imaging ONE WORLD' series, the focus of these lectures is on microscopy and image analysis methods and how to apply these to your research. Almost all aspects of imaging such as sample preparation, labelling strategies, experimental workflows, ‘how-to’ image and analyse, as well as facilitating collaborations and inspiring new scientific ideas will be covered. Speakers will be available for questions and answers. The organisers, CRUK CI core facility staff, Gurdon Institute, MRC-LMB, MRC Cancer Unit and NPL will be able to continue the discussion and provide advice on your imaging projects.


Speaker

  • Pantazis Periklis.jpg

    Periklis Pantazis

    Imperial College London
    Periklis (Laki) Pantazis is a Reader in Advanced Optical Precision Imaging (equiv. Associate Professor) at the Department of Bioengineering at Imperial College London. He studied Biochemistry at the Leibniz University of Hannover, Hannover/Germany followed by a PhD in Biology and Bioengineering at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden/Germany. He pursued then postdoctoral studies at the California Institute of Technology/Pasadena/CA/USA before joining as an Assistant Professor the ETH Zurich Department of Biosystems Science and Engineering in Basel/Switzerland. In 2018/2019, he established his Laboratory of Advanced Optical Precision Imaging at Imperial College London that conceives and applies cutting-edge imaging technologies, assays and reagents for the mechanistic dissecting of development, disease progression and tissue regeneration.

Speaker Abstract

In 2015, a novel way to convert photoconvertible fluorescent proteins was reported that uses the intercept of blue and far-red light instead of traditional violet or near-UV light illumination. In this talk I will describe and contrast this distinct two-step mechanism termed primed conversion with traditional photoconversion. I will provide a comprehensive overview of what is known to date about primed conversion and focus on the molecular requirements for it to take place. I will provide examples of its application to axially confined photoconversion in complex tissues as well as super-resolution microscopy. Further, I will describe why and when it is useful, including its advantages and disadvantages, and give an insight into potential future development in the field.