infocus Library

8 March 2022 March 2022

2021 Summer Studentship Report: Localisation and distribution of the activity regulated cytoskeleton associated protein (Arc) in the mammalian brain

During this internship, I learnt important techniques in sample preparation of brain tissue for microscopy and the use of widefield as well as confocal microscopes to visualise the anatomical distribution of proteins in the brain.

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8 March 2022 March 2022

Microscopy in the Third Age – You are never too old to Learn!

For as long as I can remember I’ve been involved with microscopy in one way or another. As a teacher I enjoyed the opportunity of being able to encourage youngsters of all ages to effectively use microscopes in the classroom setting.

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8 March 2022 March 2022

Lynne Joyce interview

In this issue, infocus speaks to biologist Dr Lynne Joyce about her career in science and microscopy – including her time at the Lord Rank Research Centre and Agar Scientific Ltd.

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8 March 2022 March 2022

Microscopy approaches in zebrafish

In many scientific fields, research is reliant on microscopy to acquire, describe, and model fundamental processes. With this need for microscopy and multitudes of microscopy techniques existing it can be challenging to identify the most suitable technique for a specific purpose. We here examine microscopy techniques to study cell fate control, vision development, and neuronal circuits covering various temporal, spatial, and computational scales in zebrafish.

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8 March 2022 March 2022

2021 Summer Studentship Report: Image processing and analysis methods for the detection of islets in Mesolens images of whole mouse pancreas specimens

The aim of this project was to develop an image processing and analysis pipeline to identify and quantify islets in confocal fluorescence Mesolens images of whole mouse pancreas specimens.

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8 March 2022 March 2022

Atomic Force Microscopy combined with Fluorescence Lifetime Imaging Microscopy (AFM + FLIM): a powerful approach to explore the structure and dynamics of biological membranes

Atomic force microscopy (AFM) and fluorescence lifetime imaging microscopy (FLIM) are two powerful techniques that are often used in the biophysical community as a means of characterising biological membranes.

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8 March 2022 March 2022

2021 Summer Studentship Report: High throughput imaging and analysis for studying feeding and digestion in a marine invertebrate model

The “Multiscope” (also known as the Rapid Access Parallel microscope) system enables multiple fields of view to be relayed to the same detector providing applications in high-throughput imaging.

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8 March 2022 March 2022

At the interdisciplinary heart of the matter: The RMS Engineering and Physical Sciences Committee

The versatility of advanced microscopy and spectroscopy is demonstrated by the wide range of applications that span across the Materials Science/Life Science boundary.

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8 December 2021 December 2021

UV transmission microscopy – optical imaging without glass

Ultraviolet (UV) transmission microscopy is a technique which has been around for over 100 years. Originally developed in the pursuit of resolution, it has become less used over time due to the technical complexities involved and the rise of other methods.

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8 December 2021 December 2021

LED microscopy illumination: a win-win for performance and sustainability

LED illumination systems offer significant performance advantages compared to traditional metal halide and mercury lamps, but did you know they are also much better for the environment? This is largely down to two factors: saving energy and avoiding mercury.

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