Bioimaging Facility, University of Edinburgh

The Bioimaging facility is based in the School of Engineering at the University of Edinburgh. We specialise in label-free microscopy methods applied to biomedical and materials research. The facility is equipped with a Bio-AFM, confocal Raman microscope and a multi-photon microscope for CARS/SRS and SHG imaging. We offer fully assisted imaging sessions, advice on experimental design and image analysis. The facility is open to external users from academia and industry.



Expertise

Imaging Platforms

Keywords - Light Microscopy: Atomic Force Microscopy | Widefield Microscopy | Multi-photon Microscopy | Raman Imaging

We currently have three imaging platforms available:

1. Label-free multi-photon microscope
A custom built multiphoton microscope with a picosecond laser system for fast coherent Raman imaging over the 1600-3600 cm-1 range.

Suitable for a combination of imaging modes:
- Coherent anti-Stokes Raman scattering (CARS)
- Stimulated Raman scattering (SRS)
- Second harmonic generation (SHG)
- Two Photon Excitation Fluorescence (TPEF)

2. InVia confocal Raman microscope (Renishaw)
Raman spectroscopy using a 785nm laser for excitation. Raman mapping capability by scanning the sample. Upright brightfield microscope for sample visualisation.

3. AFM Bioscope II (Veeco/ Bruker)
Capable of measurements in air and liquid using contact, tapping and force modulation modes. Suitable for live cell imaging and topography measurements at nanometer resolution. Can be used to measure the mechanical characteristics of the sample (e.g. Young's modulus)

Applications

Keywords – Biological: Cell Biology | Developmental Biology | Plant Biology | Neurobiology | Immunobiology | Drosophila | Bio-materials
Keywords - Physical Sciences: Bioengineering & Biomaterials | Geology/Mineralyogy | Optical & Photonic Materials and Devices | Catalysts | Nanomaterials | Composites | Magnetics | Magnetic Materials & Spintronics

Our facilities have been used to study a wide range of samples such as live stem cells, organoids, Biopsy tissues, Drosophila, engineered tissue scaffolds and fossils.

Sample Preparation

Keywords - Biological: Immunofluorescence | H&E Staining | Live Cell

As the techniques employed are label-free minimal sample preparation steps are required. We can provide tissue culture facilities for live cell imaging projects. Additional sample preparation facilities can be arranged on request.

Data Analysis

Keywords - Software: ImageJ | Fiji | Matlab

The facility manager can provide guidance on data analysis using open source software packages.

Shared Access

Bookings can be made by emailing the facility manager. Microscopes are charged for use per half day.



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