Central Laser Facility- Octopus Cluster

The Central Laser Facility (CLF) is a partnership between its staff and the large number of members of UK and European universities who use the specialised laser equipment provided to carry out a broad range of experiments in physics, chemistry and biology. The OCTOPUS imaging cluster offers a range of imaging techniques including multidimensional single molecule microscopy, confocal microscopy, super resolution, optical tweezers, Bayesian Data Analysis.


Imaging Platforms

Keywords - Light Microscopy: Confocal microscopy | Superresolution microscopy | Widefield microscopy | Laser microdissection | TIRF | Live cell imaging | Multi-photon microscopy | Gated-STED | PALM | STORM | TCSPC-FLIM | FCS/FCCS | Atomic Force Microscopy | Structured Illumination Microsocpy | Raman imaging
Keywords - Electron Microscopy: Transmission EM | Scanning Transmission EM
Keywords - X-Ray Microscopy: MicroCT

Multidimensional TIRF single molecule microscopy, confocal, multiphoton microscopy, super resolution (STORM, PALM, SIM, STED), optical tweezers, Bayesian Data Analysis
Further access to TEM, SEM, Raman microscopy, AFM, MicroCT


Keywords - Biological: Cell Biology | Plant Biology| Microbiology | Bio-materials | Food
Keywords - Physical Sciences: Bioengineering and biomaterials | Electron Optics | Functional oxides | Semiconductors and optoelectronics | Nanomaterials (nanoparticles, 2D nanomaterials, nanowires and nanotubes) | Geology / mineralogy | Optical and photonic materials and devices | Catalysts | Polymers and organic electronics | Ionic conductors, fuel cells and batteries

Molecular biology, chemical sceince, materials, catalysis

Sample Preparation

Keywords - Biological: Resin embedding | Ultrathin sectioning | Serial sectioning | Cryosectioning | Immunolabelling | Enzyme histochemistry | Live Cell

Class I &II Biolabs, Chemical labs, optics rooms and microscopy stations

Data Analysis

Keywords - Software: ImageJ | FIJI | Huygens | ImagePro | HR-STEM simulation packages (various) | Other Image simulation for crystals | Custom software
Keywords - Hardware: e-Science at RAL for large data storage

Bayesian Data Analysis, Huygens software for deconvolution


Access via facility access panel review and discussions with link scientists and programme area managers

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