Central Laser Facility- Octopus Cluster

The Central Laser Facility (CLF) is a partnership between its staff and the large number of members of UK and European universities who use the specialised laser equipment provided to carry out a broad range of experiments in physics, chemistry and biology. The OCTOPUS imaging cluster offers a range of imaging techniques including multidimensional single molecule microscopy, confocal microscopy, super resolution, optical tweezers, Bayesian Data Analysis.



Expertise

Imaging Platforms

Keywords - Light Microscopy: Confocal microscopy | Superresolution microscopy | Widefield microscopy | Laser microdissection | TIRF | Live cell imaging | Multi-photon microscopy | Gated-STED | PALM | STORM | TCSPC-FLIM | FCS/FCCS | Atomic Force Microscopy | Structured Illumination Microsocpy | Raman imaging
Keywords - Electron Microscopy: Transmission EM | Scanning Transmission EM
Keywords - X-Ray Microscopy: MicroCT

Multidimensional TIRF single molecule microscopy, confocal, multiphoton microscopy, super resolution (STORM, PALM, SIM, STED), optical tweezers, Bayesian Data Analysis
Further access to TEM, SEM, Raman microscopy, AFM, MicroCT

Applications

Keywords - Biological: Cell Biology | Plant Biology| Microbiology | Bio-materials | Food
Keywords - Physical Sciences: Bioengineering and biomaterials | Electron Optics | Functional oxides | Semiconductors and optoelectronics | Nanomaterials (nanoparticles, 2D nanomaterials, nanowires and nanotubes) | Geology / mineralogy | Optical and photonic materials and devices | Catalysts | Polymers and organic electronics | Ionic conductors, fuel cells and batteries

Molecular biology, chemical sceince, materials, catalysis

Sample Preparation

Keywords - Biological: Resin embedding | Ultrathin sectioning | Serial sectioning | Cryosectioning | Immunolabelling | Enzyme histochemistry | Live Cell

Class I &II Biolabs, Chemical labs, optics rooms and microscopy stations

Data Analysis

Keywords - Software: ImageJ | FIJI | Huygens | ImagePro | HR-STEM simulation packages (various) | Other Image simulation for crystals | Custom software
Keywords - Hardware: e-Science at RAL for large data storage

Bayesian Data Analysis, Huygens software for deconvolution

Funding

Access via facility access panel review and discussions with link scientists and programme area managers



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