Imaging ONEWORLD - 'Emerging imaging technologies to study cell architecture, dynamics and function'
12 April 2021
This week will feature Jennifer Lippincott-Schwartz from Janelia Research Campus.
Scientific Organisers: Stefanie Reichelt, Alex Sossick, Nick Barry, Alessandro Esposito and Kirti Prakash
The meeting will begin at 13:00GMT.
As part of the 'Imaging ONEWORLD' series, the focus of these lectures is on microscopy and image analysis methods and how to apply these to your research. Almost all aspects of imaging such as sample preparation, labelling strategies, experimental workflows, ‘how-to’ image and analyse, as well as facilitating collaborations and inspiring new scientific ideas will be covered. Speakers will be available for questions and answers. The organisers, CRUK CI core facility staff, Gurdon Institute, MRC-LMB, MRC Cancer Unit and NPL will be able to continue the discussion and provide advice on your imaging projects.
Janelia Research Campus
Dr. Jennifer Lippincott-Schwartz is a Senior Group Leader at the Howard Hughes Medical Institute’s Janelia Research Campus. She has pioneered the use of green fluorescent protein technology for quantitative analysis and modelling of intracellular protein traffic and organelle dynamics in live cells and embryos. Her innovative techniques to label, image, quantify and model specific live cell protein populations and track their fate have provided vital tools used throughout the research community. Her own findings using these techniques have reshaped thinking about the biogenesis, function, targeting, and maintenance of various subcellular organelles and macromolecular complexes and their crosstalk with regulators of the cell cycle, metabolism, aging, and cell fate determination. She is an elected member of the National Academy of Sciences, the National Academy of Medicine, the American Society of Arts and Sciences and the European Molecular Biology Organization. She is also a Fellow of The Biophysical Society, The Royal Microscopical Society and The American Society of Cell Biology. Her awards include the E.B. Wilson Medal of the American Society of Cell Biology, the Newcomb Cleveland Prize of the American Association for the Advancement of Science, the Van Deenen Medal, the Keith Porter Award of the American Society of Cell Biology, the Feodor Lynen Medal, and the Feulgen Prize of the Society of Histochemistry. She co-authored of the textbook “Cell Biology” and was President of the American Society of Cell Biology. Dr. Lippincott-Schwartz attended Swarthmore College, received her MS from Stanford University, and obtained her PhD in Biochemistry from Johns Hopkins University in 1986.
Powerful new ways to image the internal structures and complex dynamics of cells are revolutionizing cell biology and bio-medical research. In this talk, I will focus on how emerging fluorescent technologies are increasing spatio-temporal resolution dramatically, permitting simultaneous multispectral imaging of multiple cellular components. In addition, results will be discussed from whole cell milling using Focused Ion Beam Electron Microscopy (FIB-SEM), which reconstructs the entire cell volume at 4 voxel resolution. Using these tools, it is now possible to begin constructing an “organelle interactome”, describing the interrelationships of different cellular organelles as they carry out critical functions. The same tools are also revealing new properties of organelles and their trafficking pathways, and how disruptions of their normal functions due to genetic mutations may contribute to important diseases.