Imaging ONEWORLD - 'Exploring molecular organization and orientation in living cells using fluorescence anisotropy imaging' - Professor Satyajit Mayor
Scientific Organisers: Stefanie Reichelt, Alex Sossick, Nick Barry, Alessandro Esposito and Kirti Prakash
The meeting will begin at 1pm BST.
As part of the 'Imaging ONEWORLD' series, the focus of these lectures is on microscopy and image analysis methods and how to apply these to your research. Almost all aspects of imaging such as sample preparation, labelling strategies, experimental workflows, ‘how-to’ image and analyse, as well as facilitating collaborations and inspiring new scientific ideas will be covered. Speakers will be available for questions and answers. The organisers, CRUK CI core facility staff, Gurdon Institute, MRC-LMB, MRC Cancer Unit and NPL will be able to continue the discussion and provide advice on your imaging projects.
Satyajit Mayor serves as director of the National Centre for Biological Sciences- TIFR, Bangalore. Mayor is the former director of the Institute for Stem Cell Biology and Regenerative Medicine (inStem) at Bangalore which has a focus on the study of stem cell and regenerative biology.
Mayor studied chemistry at the Indian Institute of Technology Bombay and was awarded his PhD in life sciences from The Rockefeller University, New York. He worked as a post-doctoral fellow at Columbia University, where he developed tools to study the trafficking of membrane lipids and GPI-anchored proteins in mammalian cells using quantitative fluorescence microscopy.
Mayor started his laboratory at NCBS in 1995 after the completion of his post-doctoral research at Columbia University.
"The broad aim of Prof Mayor’s laboratory is to provide an understanding of the organization of membrane and mechanisms of endocytosis at different scales in living metazoan cells. At the molecular scale, his group seeks to uncover the organization of molecules in the membrane and molecular players in endocytic processes at the nanoscale; at the mesoscopic scale research in his laboratory attempts to provide a physical description of cell membrane structure and organization process and its material properties; at the cellular scale the work is aimed at synthesizing a role for membrane organization and endocytosis in cellular signalling and cell surface homeostasis; at the scale of the tissue the group wishes to determine how membrane organization and control of endocytosis impinges on many developmental programs in tissue morphogenesis (http://www.ncbs.res.in/mayor)."
Professor Mayor is the recipient of several national and international awards and fellowships such as the Wellcome Trust International Senior Research Fellowship, Shanti Swarup Bhatnagar Award and the JC Bose Fellowship from the DST, as well as the Margdarshi Fellowship from the DBT-Wellcome Trust India Alliance. In 2012, Mayor won the Infosys Prize for life sciences for his study of regulated cell surface organization and membrane dynamics, and was elected as a Fellow to EMBO (2013), the US National Academy of Science (2015) and TWAS (2020). He was also conferred Chevalier de l'ordre national du mérite (National Order of Merit, France), in 2016.
The organization of components of the cell has pre-occupied researchers ever since we have been able to visualize the cell and identify its constituents. A living cell is compatible with exposure to electromagnetic radiation in the visible range. With the advent of better optics and more sensitive imaging microscopes, this has allowed the visualization of sub-cellular features at the resolution of visible light in the living cell, and made the imaging of organelles quite accessible. However visualization of molecular organization in the living cell has proven challenging until the advent of super resolution imaging methods in recent years. Here I will describe an imaging modality that we have deployed in my laboratory that utilizes fluorescence emission anisotropy to uncover nano and mesoscale organization as well as molecular orientation of components in a living cell membrane. This has provided fundamental insights into the nature of organization of the living cell membrane.