Imaging ONEWORLD - 'High-speed 3D imaging with Multifocus Microscopy' - Sara Abrahamsson and Eduardo HIrata

Scientific Organisers: Stefanie Reichelt, Alex Sossick, Nick Barry, Alessandro Esposito and Kirti Prakash

The meeting will begin at 1pm BST.

As part of the 'Imaging ONEWORLD' series, the focus of these lectures is on microscopy and image analysis methods and how to apply these to your research. Almost all aspects of imaging such as sample preparation, labelling strategies, experimental workflows, ‘how-to’ image and analyse, as well as facilitating collaborations and inspiring new scientific ideas will be covered. Speakers will be available for questions and answers. The organisers, CRUK CI core facility staff, Gurdon Institute, MRC-LMB, MRC Cancer Unit and NPL will be able to continue the discussion and provide advice on your imaging projects.


  • Assistant Professor Sara Abrahamsson

    University of California, Santa Cruz
    Sara works in optical systems design and diffractive optics nanofabrication with a special interest in developing new imaging systems for super-resolution 3D microscopy in Biology. She has developed the imaging methods aberration-corrected Multifocus Microscopy and MultiFocus Structured Illumination Microscopy which enable live simultaneous 3D imaging at and beyond the diffraction limit of resolution. 

  • Ph.D. candidate Eduardo Hirata

    University of California Santa Cruz 
    Eduardo Hirata is a Ph.D. candidate in the SaraLab working with optical design and image reconstruction in 3D microscopy. Candidate Hirata is currently launching his 25-camera Multifocus Microscope which is employed in functional neuronal imaging.


Speaker's Abstract

Our research group is happy to present the unique imaging systems Multifocus SIM and M25. These optical systems are based on diffractive Fourier optics. Our lab designs and fabricates diffractive optics components with unique capabilities that multiplex and refocus the light from a microscope objective to enable simultaneous imaging of entire 3D volumes. This allows biologists to image dynamic processes such as transcription and neuronal signaling in living specimens in 3D at high speed and with high resolution.