Imaging ONEWORLD - Dr Caterina Strambio-De-Castillia 28 February 2022


Scientific Organisers: Stefanie Reichelt, Alex Sossick, Nick Barry, Alessandro Esposito and Kirti Prakash

The meeting will begin at 13:00BST.

As part of the 'Imaging ONEWORLD' series, the focus of these lectures is on microscopy and image analysis methods and how to apply these to your research. Almost all aspects of imaging such as sample preparation, labelling strategies, experimental workflows, ‘how-to’ image and analyse, as well as facilitating collaborations and inspiring new scientific ideas will be covered. Speakers will be available for questions and answers. The organisers, CRUK CI core facility staff, Gurdon Institute, MRC-LMB, MRC Cancer Unit and NPL will be able to continue the discussion and provide advice on your imaging projects.


Speaker

  • Dr. Caterina Strambio De Castillia

    Assistant Professor in Molecular Medicine at the University of Massachusetts Medical School

    Dr. Strambio-De-Castillia is a faculty member at UMass Medical School and a Chan Zuckerberg Initiative Imaging Scientist. She has multifaceted training spanning cellular biology, biochemistry, and mass spectrometry, virology, electron and light microscopy, computer science, and bio-image informatics. She has an ongoing interest in elucidating the fundamental principles governing the intracellular trafficking of viruses, the dynamic regulation of nucleocytoplasmic transport as it interfaces with nuclear envelope structure and with chromatin organization and function. More recently, she spearheaded several multi-disciplinary bio-image informatics projects and initiatives aimed at promoting scientific rigor, reproducibility, and the FAIR exchange of data through the standardization of metadata guidelines and quality-control procedures, and the development of shared software infrastructure for imaging pipelines.

Abstract

For quality, interpretation, reproducibility and sharing value, microscopy images should be accompanied by detailed descriptions of the conditions that were used to produce them. In this talk we will discuss highly interoperable, open-source software tools that were designed in the context of burgeoning global bioimaging community initiatives to facilitate the documentation of microscopy experiments as specified by the recent 4DN-BINA-OME tiered-system of Microscopy Metadata specifications. In addition to substantially lowering the burden of quality assurance, these tools visual nature of these tools make them well suited for teaching users about the intricacies of image acquisition and how it impacts the results of their experiments.