Cell Imaging Techniques Course 2012

The Course was of immense value to any life scientist or cell biologist, from research or technical background, from hospitals, research institutes, industry or academia and at any stage in their career, who wants to learn or update their knowledge of current cell imaging techniques, either for routine purposes or for research.


2012 Programme

Monday 23rd July

09.30 - 09.45     Welcome Susan Brooks
09.45 - 10.45     Presentation: General introduction to cell imaging - John Runions 
10.45 - 11.00     Tea and coffee
11.00 - 12.00     Presentation: Introduction to antibodies and to LM immunocytochemistry -Susan Brooks TAB 2
12.00 - 13.00     Practical set up – Affinity labelling of tissue sections and cells grown on coverslips - Susan Brooks / Pippa Hawes
13.00 - 14.00     Lunch (and antibody incubation period)
14.00 - 16.00     Practical continues – Affinity labelling of tissue sections - Susan Brooks / Pippa Hawes
16.00 - 16.30     Tea & coffee
16.30 - 17.30    Presentation: Introduction to light and fluorescence microscopy  - John Runions 

Tuesday 24th July

09.00 - 09.30    Presentation: Labelling cells for Confocal microscopy - Pippa Hawes
09.30 - 10.00     Practical – Immunofluorescence labelling - Jenny Simpson
10.00 - 10.30     Presentation: Preparation of tissue sections for Confocal microscopy - Pippa Hawes 
10.30 - 11.00     Tea and coffee
11.00 - 13.00     Workshop – Imaging fluorescent specimens - Pippa Hawes, John Runions, Mark Fricker
13.00 - 14.00     Lunch
14.00 - 16.30     Workshop – Imaging fluorescent specimens continues - Pippa Hawes, John Runions, Mark Fricker
16.30 - 17.30     Presentation: Fluorescent proteins and live cell imaging - Mark Fricker 

Wednesday 25th July Live Cell Imaging Day

09.30 - 11.00     Workshops - Live cell imaging - John Runions, Pippa Hawes
11.00 - 11.15     Tea and coffee
11.15 - 13.00     Workshops - Live cell imaging - John Runions, Pippa Hawes
13.00 - 14.00     Lunch
14.00 - 15.30     Workshops - Live cell imaging
15.30 - 16.00     Tea and coffee
16.00 - 17.00     Presentation: Introduction to EM - Pippa Hawes 

Thursday 26th July

09.00 - 09.30     Practical – Antibody labelling of EM Sections - Pippa Hawes 
09.30 - 10.30     Presentation: Introduction to immunogold labelling - Chris Hawes 
10.30 - 10.45     Coffee
10.45 - 12.00     Practical – Contrasting Sections for EM - Pippa Hawes
12.00 - 13.00     Presentation: Electron tomography - Sue Vaughan
13.00 - 14.00     Lunch
14.00 - 15.30     Practical – Imaging grids by EM - Pippa Hawes
Demonstration – Cryosectioning for EM - Gareth Morgan / Gareth Jackson
Demonstration – High Pressure Freezing Barry Martin
15.30 - 16.00     Tea
16.00 - 17.00     Cell Biology Section Annual Lecture 'walking to the ends, discovering howmolecular motors work in cells' - Michele Peckham

Friday 27th September

09.30 - 10.30     Presentation: Introduction to FISH - Emanuela Volpi 
10.30 - 10.45     Coffee
10.45 - 11.45     Presentation: Introduction to image analysis - Paul Sheppard 
11.45 - 12.00     Course Assessment and farewell - Susan Brooks

 



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