Monday 14 September
14:00 – 14:30 BST
Introduction from tutors and delegates; Overview of course

General

14:30 – 14:40 BST
Techno Bites

Techno Bite

14:45 – 15:00 BST
Basics of software – importing data, plots and gating

Talk

15:00 – 15:45 BST
Viability dyes

Talk

15:45 – 16:45 BST
DNA analysis (PI, Hoechst, BrdU)

Talk

16:45 – 17:00 BST
Q&A, summary and wrap-up

General

Tuesday 15 September
14:00 – 14:15 BST
Introduction from tutors and delegates; Overview of course

General

14:15 – 14:30 BST
Techno Bites

Techno Bite

14:30 – 14:45 BST
Basics of software - importing data, plots and gating

Talk

14:45 – 15:30 BST
Manual compensation using phenotyping data

Talk

15:30 – 16:00 BST
Automatic compensation

Talk

16:00 – 16:30 BST
Analysis of stained samples including FMO

Talk

16:35 – 17:00 BST
Q&A, summary and wrap-up

General

Research Stream

Wednesday 16 September
14:00 – 14:15 BST
Introduction from tutors and delegates; Overview of course

General

14:15 – 15:15 BST
FCSExpress HD analysis

Talk

15:15 – 15:20 BST
Techno Bite

Techno Bite

15:20 – 16:20 BST
FlowJo HD analysis

Talk

16:20 – 16:50 BST
Quantification

Talk

16:50 – 17:00 BST
Q&A, summary and wrap-up

General

Clinical Stream

Wednesday 16 September
09:00 – 10:00 BST
Discussion and feedback about pre-recorded talks

Talk

10:00 – 10:30 BST
Discussion about general laboratory methodology, sample preparation, antibody panels etc.

Talk

10:30 – 11:00 BST
Coffee Break

General

11:00 – 12:30 BST
Concepts of flow cytometry data analysis - gating strategies, illustrated with patient cases

Talk

12:30 – 13:30 BST
Lunch Break

General

13:30 – 15:00 BST
Live data analysis using Kaluza/Diva

Talk

15:00 – 15:30 BST
Coffee Break

General

15:30 – 17:00 BST
Live data analysis using Kaluza/Diva

Talk

Thursday 17 September
14:00 – 14:15 BST
Introduction from tutors and delegates; Overview of course

General

14:15 – 14:30 BST
Techno Bite

Techno Bite

14:30 – 15:00 BST
Proliferation – dye dilution

Talk

15:00 – 16:00 BST
Apoptosis

Talk

16:00 – 16:30 BST
Calcium flux

Talk

16:35 – 17:00 BST
Q&A, summary and wrap-up

General