Prime Editing Off-Target Seq
Unlike conventional CRISPR-Cas9 nucleases, prime editors operate through a unique mechanism involving a nickase-reverse transcriptase fusion and programmable pegRNA. This fundamental difference means that traditional off-target detection methods—designed to identify double-strand breaks—may fail to capture the full spectrum of unintended prime editing events.
Created: 20 Sep 2026 09:09:49 AM
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