The latest in a long running series of RMS sponsored meetings dating back to the 1960s & the heyday of electron microscopy. Recently the meetings have been re-branded to encompass all aspects of bioimaging relating to modern plant cell biology.

The programme was constructed from key note talks by top class plant scientists and selected talks from submitted abstracts.

Programme

Sunday 19th April    

1700    Registration in Holland Hall
1830    Opening Reception and Dinner

Monday 20th April

0800 – 0900    Late registration at PCCC
    
0900 – 0915    Introduction and Welcome

0915 – 1000    Peter Hepler - Calcium, pH and Secretion in pollen tubes: Which appears first after inhibition of growth?

Session 1    Endomembrane system and the plasma membrane 

1000 – 1045    Invited Talk: Daniel van Damme - The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants
    
1045 – 1105    Tea and Coffee 
    
1105 – 1130    Pengwei Wang - Interactions of plant cytoskeleton, endoplasmic reticulum and plasma membrane mediated by NET and VAP27 proteins 

1130 – 1155    Lawrence Griffing - Lattice light sheet and confocal microscopy of how cytoplasmic microtubules, tip actin, and myosin XI control the distinct form of the endoplasmic reticulum during tip growth in Physcomitrella patens

1155 – 1220    Tijs Ketelaar - Localization and functions of the vesicle tethering exocyst complex in plants
    
1220 – 1430    Lunch

Session 2    Organelles and Interactions

1430 – 1515    Invited Talk: Jaideep Mathur - Photo-convertible fluorescent proteins provide fresh insights on sub-cellular interactions in plants

1515 – 1540    Imogen Sparkes - Using optical tweezers to probe for physical connections between organelles

1540 – 1605    Mistianne Feeny - On the heels of the elusive protein storage vacuole: developing strategies to study vacuole biogenesis in Arabidopsis thaliana

1605 – 1630    David Collings - Characterisation of putative nuclear pore-anchoring proteins in Arabidopsis thaliana
    
1630 – 1650    Tea and Coffee 
    
1650 – 1715    Verena Kriechbaumer - Reticulomics: Fish n' Tell

1715 – 1740    David Logan - The Clu-type protein, FRIENDLY, regulates mitochondrial distribution, fusion, and quality control in Arabidopsis  

Session 3    Cell Walls sponsored by Quorum Technologies

1740 – 1805    Kris van’t Klooster - Cortical microtubules determine when and where secondary cell wall thickenings are deposited

1805 – 1850    Invited Talk: David Erhardt - Beyond the MTOC: Mechanisms for cortical microtubule organization in higher plants
    
1900    Dinner at Holland Hall

Tuesday 21st April

Session 3 cont…    Cell Walls   

0900 – 0925    Rosemary White - Characterising mutants in root-to-shoot mobile silencing in Arabidopsis

0925 – 0950    Joe Mckenna - Across the great divide: The plant cell wall, plasma membrane and cytoskeleton

0950 – 1015    Ulla Neumann - Differential pectin methylesterification in fruits with explosive versus non-explosive pod shatter

Session 4    Cytoskeleton   

1015 – 1100    Invited Talk: Gohta Goshima - Functions of microtubules and motors in plant cells: A live-cell microscopy approach using the moss Physcomitrella patens
    
1100 – 1120    Coffee and Exhibition
    
1120 – 1145    Einat Sadot - A Remorin that cosegregates with myosin VIII in plasma membrane microdomains and colocalizes with microtubules

1145 – 1210    Ingeborg Lang - Dynamic reactions of the cortical cytoskeleton in a plasmolytic cycle

1210 – 1235    Jeroen de Keijzer - Control and function of microtubule overlaps in the bipolar phragmoplast network
    
1235 – 1250    Steve Murray, Hitachi High Technologies GmbH - It's Ionic, but you don't need VP
    
1250 – 1400    Lunch and Exhibition
    
Lunch was kindly sponsored by Carl Zeiss

1400 – 1600    Boat Trip/Free Time
1900    Poster Session, Buffet and Exhibition    

Wednesday 22nd April

Session 5    Techniques and Applications   

0900 – 0945    Invited Talk: Alexis Maizel - Quantitative analysis of lateral root morphogenesis using light sheet microscopy

0945 – 1010    Moritz Nowack - Live and Death Cell Imaging – Analysis of Programmed Cell Death Processes in Plant Development

1010 – 1035    Ton Timmers - Looking inside plant root nodules with Clarity
    
1035 – 1055    Coffee and Exhibition
    
1055 – 1140    Invited Talk: Ursula Luetz-Meindl - Structural stress responses of dictyosomes in algae analyzed by TEM and FIB-SEM tomography

1140 – 1205    Maike Kittleman - Serial block face SEM reveals an interconnected organelle network in plant cells

1205 – 1230    Clair Collins - Advances in Elemental Analysis & Mapping of Botanical Samples in the SEM
    
1230 – 1430    Lunch and Exhibition – UK BioImaging discussion 1330-1430
    
Lunch was kindly sponsored by Carl Zeiss

1430 – 1455    Anja Geitmann - Taking micro-manipulation to the next level: Combining live cell imaging and  Lab-on-Chip technology

1455 – 1520    Tomas Nahlik - Expertomica 3D Reconstruction and Volume Measurement Tool - NARA: a new software for analysis of fluorescent images

1520 – 1540    Tea and Coffee   

Session 6    Host-Pathogen Interface

1540 – 1625    Invited Talk: Francine Govers - Dynamics of the actin cytoskeleton in hyphae and infection structures of the oomycete pathogen Phytophthora infestans

1625 – 1650    Mike Deeks - Reconfiguring plant cell polarity in response to pathogens 

1650 – 1715    Mark Fricker - ROS, redox and pathogenicity in rice blast disease

1715 – 1800    Guest Talk: Rob Kesseler - Convergent Territories - new opportunities in Art & Science
    
1900    Conference Photo (Reed Hall Steps)
1900    Conference Dinner Reception Drinks
2000    Dinner   

Thursday 23rd April   

Session 6 cont…    Host-Pathogen Interface

0900 – 0945    Invited Talk: Hannah Kuhn - Traffic control for plant immunity and receptor kinases 
    
0945 – 1010    Jelle Postma - The Cf-4 receptor-like protein recruits the BAK1 co-receptor to initiate receptor endocytosis and plant immunity

1010 – 1035    George Littlejohn - The switch from biotrophic to necrotrophic growth of Magnaporthe oryzae during  infection of rice is dependent on local environmental conditions and may be characterised by the localisation of effectors and host cell cytoplasmic components
    
1035 – 1100    Tea and Coffee 
    
1100 – 1125    Janice de Almeida Engler - Ectopic expression of cell cycle inhibitor genes interferes with root-knot nematode feeding site development

1125 – 1150    Kathryn Wright - The endophytic lifestyle of Escherichia coli O157:H7: internal localisation and proliferation

1150 – 1215    Elysa Overdijk - A novel pathosystem between Phytophthora and the moss Physcomitrella patens gives insight into cellular plant defence responses
    
1215 – 1230    Closing remarks by organisers
1230 – 1430    Lunch and depart