The hands-on CLEM course will cover three correlative workflows - 3D CLEM, Integrated Light and EM, and cryo CLEM - using a range of techniques and equipment including Near Infrared Branding, micro CT, Serial Block Face SEM, Focused Ion Beam SEM, high pressure freezing, in-resin fluorescence, ultraLM, integrated light and SEM (SECOM), plunge freezing and cryo-fluorescence. There will also be a CLEM image analysis tutorial session. There are limited places available on the CLEM course. 

Course Information

There are 9 spaces on this course

Cost: £150

Venue: Francis Crick Institute, 1 Midland Road, London, NW1 1AT

Application process: CV and covering letter should be emailed to Raffa Carzaniga ([email protected]). The covering letter should include a statement of motivation for attending the course, how the workshop will impact your research, and whether you plan to train others in your home institute.

Deadline for applications: 30^th April 2019.

Decision: 13^th May 2019.

Course requirements:

Participants should have a working knowledge of the fundamentals of light and electron microscopy.

Participants should bring a laptop, and are encouraged to bring a correlative dataset in case there is time to troubleshoot individual cases.

Participants should not bring their own samples, demonstration specimens will be provided.

Accommodation: To be booked by applicants, we will provide a list of local hotels.

Subsistence: Coffee and lunch will be provided on the Wednesday, Thursday and Friday. Dinner will be provided on Wednesday and Thursday.

Note: Priority will be given to applicants working at UK institutions, to fill a training gap, since the EMBO CLEM Course takes mainly international participants.

Workflows

Workflows:

All participants will take part in all workflows, rotating over three days.

1. 3D Correlative Light and Electron Microscopy

Confocal of cells on gridded coverslips (Tutorial)

Near Infra-Red Branding (Hands-On)

NCMIR ‘megametal’ sample processing (bench and microwave) (Tutorial)

MicroCT (Demo)

Sample mounting (Hands-On)

Serial Block Face SEM set-up, with Focal Charge Compensation and SBEM Image (Demo)

2. Integrated Light and Electron Microscopy

High Pressure Freezing (Hands-On)

In-Resin Fluorescence freeze substitution (Hands-On)

Trimming (Demo)

UltraLM (Hands-On)

Quanta-SECOM (Demo)

Pick-up methods (Tutorial)

ATUMtome (Demo)

Array Tomography (Tutorial)

3. Cryo Correlative Light and Soft X-Ray Tomography

Confocal mapping of cells on grids (Tutorial)

Plunge freezing (Hands-On)

Cryo-fluorescence (Hands-On)

Cryo-soft X-Ray tomography (Tutorial)

4. Correlative Data Clinic (Evening)

Overview of correlative softwares and resources (Tutorial)