Crick Electron Microscopy Opening Symposium
Crick Electron Microscopy Opening Symposium
Organisers: Dr Lucy Collinson, Dr Peter Rosenthal, Dr Raffaella Carzaniga
The Francis Crick Institute in London will host a symposium to celebrate the opening of the new Electron Microscopy Science Technology Platforms, with national and international invited speakers presenting the cutting edge in application of imaging technology to answer critical research questions, from molecules to whole organisms. The symposium will include presentations on new frontiers in imaging and big data analysis.
The symposium will be followed by a day of hands-on workshops and demonstrations, using a wide range of the techniques and technologies housed in the new facilities.
The symposium will be accompanied by an interactive exhibition from our sponsors.
Registration for this event is now closed. If you have any queries, please contact Karina Lang.
Registration Information and Venue
Please note:
Registration on the RMS website is for attendees that are not Crick employees or partner HEIs, the registration fee is £120. You can register by using the Book Now button above and on the right if you are using a desktop.
Registration will close on 21 June 2017.
If you are a Crick employee or from a partner HEI, please register on the Crick eventbrite site
For a password, please see Cricknet or email [email protected]
Venue
The Francis Crick Institute, 1 Midland Road, London, NW1 1AT
Symposium Programme
Workshops
Crick Electron Microscopy Workshops
Registration for the workshops is free, but you must be registered for the symposium to take part. Once you have registered for the symposium, you will receive instructions on how to register for the workshops.
Friday 14th July 2017
09.15 Arrival and registration
Workshop 1: Sample Preparation: microwaves and high pressure freezing
Presentations from world leading experts in EM sample preparation, and demos of
the BioWave microwave from Ted Pella/Agar Scientific, the ICE high pressure freezer
from Leica, and an introduction to the new Light μ high pressure freezer from
CryoCapCell.
Crick host: Julia Konig
Commercial hosts: Cindy Smith (Ted Pella), Ben Hayllar (Agar Scientific), Frederic Leroux (Leica), Xavier Heilegenstein (CryoCapCell)
Invited Speakers: Kent McDonald, Rick Webb
Format: Full day, 9 attendees
09.30 – 10.30: Rick Webb
10.30 – 11.30: Kent McDonald
11.30 – 12.00: Discussion
12.00 – 13.00: Lunch
13.00 – 14.00: Rotation (BioWave/Leica ICE/ Light μ)
14.00 – 15.00: Rotation (BioWave/Leica ICE/ Light μ)
15.00 – 16.00: Rotation (BioWave/Leica ICE/ Light μ)
Workshop 2: ATUMtome
Demonstration of the ATUMtome from RMC, an automated ultramicrotome that can
collect thousands of ultrathin sections onto tape for imaging by array tomography,
originally developed by Jeff Lichtman at Harvard for high resolution volume imaging
of the brain.
Commercial host: Natalie Hackett (RMC/ISS)
Format: 4 x 1 hour sessions, 2 people in each
09.30 – 10.30: Group 1
11.00 – 12.00: Group 2
12.00 – 13.00: Lunch
13.00 – 14.00: Group 3
14.30 – 15.30: Group 4
Workshop 3: CEMOVIS
Demonstration of cryo-sectioning for Cryo-Electron Microscopy of Vitreous Sections
(CEMOVIS) using the new Diatome micromanipulators and the Leica FC7 cryoultramicrotome
Crick host: Matt Russell
Commercial host: Helmut Gnaegi (Diatome), Steve Cham (Labtech)
Format: 2 x 2 hour sessions, 3 people in each
10.00 – 12.00: Group 1
12.00 – 13.00: Lunch
13.00 – 15.00: Group 2
Workshop 4: Plunge freezing and cryo-fluorescence
Demonstration of the Leica plunge freezer and Linkam cryo-fluorescence stage, for
preparing and imaging vitreous cells for cryo-TEM and cryo-soft X-ray tomography.
Crick host: Marie-Charlotte Domart
Commercial host: Duncan Stacey and Michael Schwertner (Linkam Scientific)
Format: 2 x 2 hour sessions, 3 people in each
10.00 – 12.00: Group 1
12.00 – 13.00: Lunch
13.00 – 15.00: Group 2
Workshop 5: FEI BioTwin TEM with Gatan OneView camera
Demonstration of the new Gatan OneView camera for fast sensitive imaging of
biological samples in the TEM. Tomogram and montage.
Crick host: Raffa Carzaniga
Commercial host: Neil Wilkinson, Mark Day (Gatan)
Format: 4 x 1 hour sessions, 2 people in each
09.30 – 10.30: Group 1
11.00 – 12.00: Group 2
12.00 – 13.00: Lunch
13.00 – 14.00: Group 3
14.30 – 15.30: Group 4
Workshop 6: FEI Quanta SEM with DELMIC SECOM fluorescence microscope
Demonstration of high accuracy correlative microscopy, using the DELMIC SECOM
integrated fluorescence and electron microscope, which is capable of in-vacuum
PALM/STORM super resolution imaging.
Crick host: Chris Peddie
Format: 2 x 2 hour sessions, 3 people in each
10.00 – 12.00: Group 1
12.00 – 13.00: Lunch
13.00 – 15.00: Group 2
Workshop 7: Zeiss Sigma SEM with Gatan 3View Serial Block Face SEM
Demonstration of 3D correlative imaging of cells using Serial Block Face SEM.
Crick host: Lucy Collinson
Commercial host: Steve Furzeland (Zeiss)
Format: 2 x 2 hour sessions, 2 people in each
10.00 – 12.00: Group 1
12.00 – 13.00: Lunch
13.00 – 15.00: Group 2
Workshop 8: Zeiss Merlin SEM with Gatan 3View Serial Block Face SEM and new
OnPoint Detector
Demonstration of Serial Block Face SEM for volume imaging of brain tissue, with new
Gatan OnPoint detector.
Crick host: Carles Bosch
Commercial host: Steve Furzeland (Zeiss)
Format: 2 x 2 hour sessions, 2 people in each
10.00 – 12.00: Group 1
12.00 – 13.00: Lunch
13.00 – 15.00: Group 2
Workshop 9: Zeiss Crossbeam 540 Focused Ion Beam SEM
Demonstration of Focused Ion Beam SEM for volume imaging of cells and tissues.
Crick host: Chris Peddie / Raffa Carzaniga
Commercial host: Andreas Schertel (Zeiss)
Format: 4 x 1 hour sessions, 2 people in each
09.30 – 10.30: Group 1
11.00 – 12.00: Group 2
12.00 – 13.00: Lunch
13.00 – 14.00: Group 3
14.30 – 15.30: Group 4
Workshop 10: Zeiss Versa 510 microCT
Demonstration of X-ray imaging of large biological samples for high resolution
anatomical studies and localisation of regions of interest for 3D correlative
microscopy.
Crick host: Anne Weston
Commercial host: Rosy Manser (Zeiss)
Format: 4 x 1 hour sessions, 2 people in each
09.30 – 10.30: Group 1
11.00 – 12.00: Group 2
12.00 – 13.00: Lunch
13.00 – 14.00: Group 3
14.30 – 15.30: Group 4
Workshop 11: ultraLM
Demonstration of a new miniaturised fluorescence microscope that integrates into
ultramicrotomes for detection of fluorescent cells during sample preparation.
Crick host: Lizzy Brama
Format: 4 x 1 hour sessions, 2 people in each
09.30 – 10.30: Group 1
11.00 – 12.00: Group 2
12.00 – 13.00: Lunch
13.00 – 14.00: Group 3
14.30 – 15.30: Group 4
Workshop 12: Image analysis
Hands-on training in the use of two open softwares, for correlative microscopy and
semi-automated segmentation of EM and X-ray images.
Crick host: Martin Jones
Invited Speakers: Perrine Paul Gilloteaux (EC-CLEM), Michelle Darrow (SuRVoS)
Format: Full day, 8 attendees
09.30 – 12.30: Perrine Paul Gilloteaux (EC-CLEM)
12.30 – 13.30: Lunch
13.30 – 16.30: Michelle Darrow & Imanol Luengo (SuRVoS)
Workshop 13: FEI Krios
Crick host: Peter Rosenthal
Commercial host: Francis Morrissey (FEI) TBC
Format: 2 x 2 hour sessions, 3 people in each
10.00 – 12.00
12.00 – 13.00: Lunch
13.00 – 15.00
Workshop 14: Carbon coating and Carbon films
Demonstration of the Quorum Technologies GloCube glow discharge unit and Q150T carbon coater for making carbon support films for single particle negative staining and cryoEM.
Crick host: Raffa Carzaniga
Commercial host: Bob Morrison (Quorum Tachnologies)
Format: 2 x 1 hour sessions, 3 people in each
09.30 – 10.30: Group 1
11.00 – 12.00: Group 2
Sponsors
If you are interested in sponsoring this event, please click here.
Confirmed Sponsors
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FEI
See beyond with FEI's leading edge SEM, TEM, ESEM and DualBeam™ solutions. Our 60 year history of pushing the boundaries of microscope innovation has resulted in instruments delivering sub-nm SEM and sub-Å TEM resolution. Whatever your application in materials or life sciences, FEI delivers the highest performance solution, and puts you at the center of a global community of leading researchers and scientists.
Find out more about FEI
fei.com -
ZEISS
Carl Zeiss is an innovative technology leader in the fields of optics, precision engineering and electronic visualisation. Time and time again, we set new, pioneering standards in sophisticated technology for recognising, experiencing, measuring, analysing, structuring and processing a wide spectrum of objects. With professional optics we meet the expectations of even our most critical customers - not only in the fields of research, medicine, industry, but also for use in leisure activities.
Find out more about ZEISS
www.zeiss.com -
Gatan
Gatan is the industry leader in the research, development and manufacturing of SEM and TEM products.
For TEM, Gatan provides an unrivalled range of high performance digital imaging and analytical systems, especially EELS spectrometers and energy filters (EFTEM). For SEM, Gatan are world leaders in cathodoluminescence (CL) technology and complement this with cooling stages and EBIC products.
Gatan is striving for continuous product innovations to increase users' productivity and make electron microscopes more effective and powerful tools. Visit the website for complete information on Gatan's extensive range of products for electron microscopy.
Find out more about Gatan
www.gatan.com -
Leica UK
Leica Microsystems is a leading manufacturer and supplier of high precision optical solutions based on microscopes and related instruments. The company manufactures a comprehensive portfolio of products used in a wide variety of areas requiring vision, measurement and analysis, including applications in the life sciences (such as bio-technology research and medicine) and the material sciences.
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Agar Scientific
Agar Scientific. Microscopy, Lab and Medical supplies at your fingertips
A leading international supplier of scientific instruments and accessories for over 40 years, Agar Scientific specialises in consumables and equipment supporting all forms of microscopy. Agar Scientific provides a fast, efficient online and offline ordering service, allowing customers to purchase with ease from our extensive range of laboratory and medical supplies and equipment. Our technical support staff has a wealth of experience in specimen preparation and microscopy techniques.
Our online catalogue of microscopy products includes:
TEM Grids - Apertures - Calibration standards - SEM stubs - Specimen preparation equipment - Filaments - Tweezers - Slides & cover glasses - Vacuum coating - Fixatives and resins - EM support films - Magnifiers - Specimen storageWe welcome enquiries regarding the use of any of our products, along with any suggestions for additions to our range. Orders ship from our UK headquarters in Stansted, Essex, and through our established network of agents and distributors who provide technical and applications support worldwide.
ISO9001:2008
Agar Scientific maintains a documented quality plan specifying our manufacturing and distribution processes and is approved by SGS to ISO9001:2008.Find out more about Agar Scientific
Web: www.agarscientific.com LinkedIn: Agar Scientific Twitter: @agarscientific -
Bruker
For almost 50 years Bruker has been driven by a single goal: to provide the best technological solution for each analytical task. Today, worldwide, more than 4,000 employees in over 90 locations on all continents work towards this enduring vision.
Bruker’s drive to develop state-of-the-art technologies and innovative solutions for today’s analytical questions remains constant, evidenced by the many product lines that lead their respective markets. With one of the world’s most comprehensive ranges of scientific instrumentation available under one brand, the Bruker name is synonymous with excellence, innovation and quality.Find out more about Bruker
www.bruker.com -
DiATOME
Diamond Knives - Development, Manufacturing & Customer Service since 1970.
We offer technical assistance in all fields of ultramicrotomy, free sectioning tests for all types of samples, many years of experience in perfecting our knives. With a telephone call or an email we can inform you of any details you require. We are convinced that our future developments will allow diamond knives to be used for many new applications.
Find out more about DiATOME
www.diatome.ch -
Fischione Instruments
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Hitachi High-Technologies
At Hitachi High-Technologies our primary goal is to provide customers with powerful, dependable and easy-to-use microscope solutions for the advancement of science & engineering. Our technologically advanced solutions in SEM, TEM and FIB are developed through a long-term commitment to research and development, drawing upon the resources of the whole Hitachi group.
By employing recognised pioneers and developing long term partnerships with world-renowned authorities in electron microscopy, we are able to provide unique technologies which push the boundaries of science. Our Tabletop Microscope is making electron microscope capabilities accessible to all, whilst key technologies such as cold field emission and Cs-correction are making ultra-low voltage imaging in SEM and atomically resolved imaging and analysis in TEM/STEM a practical reality.
Find out more about Hitachi High-Technologies
www.hitachi-hightech.com/eu/ -
ISS Group Services
The ISS Group is a provider of electron optical systems, solutions and related technologies to visualise and characterise materials from the macro down to the nano scale. Whether this is the supply of the latest instrumentation technology or through training and service support of older systems, ISS offers more than 30 years of experience serving the UK and overseas markets. We represent a number of prestigious manufacturers in the UK and Ireland and continue to grow all aspects of the business.
Our friendly and knowledgeable team is available to help you answer your queries.
Find out more about ISS Group
www.iss-group.co.uk -
JPK Instruments
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Labtech International Ltd
Since 1993 Labtech International has been a leading supplier of innovative instrumentation into the UK and Ireland research markets. The electron microscopy division continues this tradition offering an expanding range of technology-leading instruments, accessories and consumables for SEM, TEM, FIB, LM and probe microscopy.
Our partners include Micro to Nano, who offer an extensive and growing range of microscopy supplies – including SEM stage and stub adaptors. We also represent DiATOME diamond knives, Kleindiek Nanotechnik nanomanipulators, Safematic sputter/carbon coaters, PIE Scientific RF plasma cleaners and from Tousimis fully automatic critical point dryers.
Labtech actively supports its customers through highly qualified teams of field-based sales, service and application specialists.
Find out more about Labtech International
www.labtech.com -
Linkam Scientific Instruments
Linkam develops and manufactures a broad range of heating and freezing stages for both OEM and end users to visualize and explore materials properties. Used in conjunction with light microscopes and other forms of spectroscopy, Linkam stages are found in thousands of laboratories worldwide with the most successful microscope heating stage, the THMS600, selling over 4,000 units alone. Linkam is the market leader in temperature controlled microscopy.
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Molecular Dimensions
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Quantifoil
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Quantum Design UK and Ireland
Quantum Design UK and Ireland (QDUKI) are a leading distributor of high-tech instrumentation and consumables for scientific, academic and industrial research. QDUKI are part of the Quantum Design International Group. Our success in distributing scientific products comes from more than 30 years’ experience in manufacturing and distributing our own industry-leading materials characterisation systems.
Please visit our website www.qd-uki.co.uk to see the Electron Microscopy instrumentation range we can offer which includes Nanomegas Electron Precession Diffraction, GETec Microscopy In-Situ AFM in SEM and SCL Self Sensing Cantilevers.
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Ted Pella
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Thistle Scientific
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Ziath Ltd
