Webinar: Live Imaging of the Extracellular Matrix With a Glycan-Binding Fluorophore
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Visualizing the extracellular matrix (ECM) in real-time has long been a challenge. Dr. Kayvon Pedra’s group has developed a novel, cell-impermeant fluorophore that red-shifts and activates upon binding glycans. This allows for seamless, wash-free imaging of glycosylated ECM architecture—from in vitro models to in vivo mouse tumors—providing a powerful new tool for live fluorescence microscopy.
Highlights of the presentation
A cell-impermeable small molecule fluorophore turns on and red-shifts upon binding glycosylated biomolecules.
Application of the dye to live biological samples enables wash-free visualization of extracellular matrix structure.
Observations demonstrate no toxicity, a broad substrate profile, deep tissue penetration, and negligible photobleaching in a variety of model organisms.
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- Amanda Jarman
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