infocus #41 March 2016 Isotropic 3D Imaging over Large Volumes with Axially Swept Light-Sheet Microscopy

The authors detail the study of biomedically important phenomena within physiological three-dimensional microenvironments.

DOI: 10.22443/rms.inf.1.134

Dean and Fiolka have developed a SPIM imaging technique, referred to as Axially Swept Light-Sheet Microscopy (ASLM), that enables high-resolution isotropic imaging of cellular processes within large and experimentally tractable 3D microenvironments. ASLM uses aberration-free remote focusing to scan a laser line focus (i.e., a narrow sheet of light) in its propagation direction synchronously with a rolling active-pixel array on a scientific CMOS camera.

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Authors

Kevin Dean

Postdoctoral Fellow, UT Southwestern Medical Centre, Dallas, Texas

Kevin Dean was raised in Northern California, received his B.A. in Chemistry at Willamette University, and completed his Ph.D. in Biochemistry at the University of Colorado. Under the guidance of Prof.Amy Palmer, his doctoral work focused on fluorescent protein spectroscopy and engineering with high-throughput multiparameter microfluidic cell sorters. Following graduation, he served as the Director of the BioFrontiers Advanced Imaging Resource. Today, his research aims to integrate advanced light-sheet microscopy, computer vision, and cell biology to understand cytoskeletal dynamics and signal transduction in 3D extracellular matrix environments.

Reto Fiolka

Instructor in Department of Cell Biology, UT Southwestern Medical Centre, Dallas, Texas.

Dr. Fiolka was born in Switzerland and studied Mechanical Engineering at ETH Zurich, where he also completed his PhD in advanced microscopy techniques involving structured illumination and diffraction tomography. He performed postdoctoral research at HHMI’s Janelia Farm research campus under the guidance of the late Mats Gustafsson on the development of live 3D structured illumination microscopy. In a second postdoctoral stay at Janelia Farm, he worked with Meng Cui on adaptive optics. At UT Southwestern, Reto Fiolka now develops imaging technologies for imaging cells in 3D microenvironments with high spatiotemporal resolution.