This two day annual confocal course utilises many different sample types and fluorescent probes (DNA stains, classic antibody labels and fluorescent proteins) which are chosen to best demonstrate particular problems and techniques. Focus is always on the techniques they enable and the problems they generate, which will be applicable to any sample types. The two days consist of short tutorials followed by hands-on practice.
Day 1 takes participants through the basic principles of confocal microscopy and then trains them, through hands-on practice, how to configure and image multicolour, multidimensional samples using a confocal microscope.
Day 2 builds on the experience of Day 1 and enables participants to try FRAP and spectral profiling.
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