Super-resolution Workshop 2017

09:00 – 17:00 BST 7 July 2017 + Add to calendar Leeds

Super-resolution Workshop 2017

09:00 – 17:00 BST 7 July 2017 + Add to calendar Leeds

Designed to talk about the current challenges in developing and using super-resolution microscopy with lots of time for discussion, this year the workshop will focus on the topic of labelling. Thoughts and ideas are encouraged to help define what is good/best practice around these challenging techniques, and give an insight into future potential developments.

Registration will close on Friday 23 June.

Programme

Speakers

Scientific Organiser

Professor Michelle Peckham

Executive Honorary Secretary

University of Leeds
Michelle is Professor of Cell Biology in the Faculty of Biological Sciences. She obtained a BA in Physiology of Organisms at the University of York, and a PhD in Physiology at University College London. She moved to King's College London, and started to use a specialised form of light microscopy (birefringence) to investigate muscle crossbridge orientation. She then worked at UCSF, San Francisco for a year, where she used fluorescence polarisation to investigate muscle crossbridges. She moved back to the UK, to the University of York, to work on insect flight muscle. In 1990 she was awarded a Royal Society University Research fellowship, based at King's College London, and began working on the cell and molecular biology of muscle development, and started to use live cell imaging to investigate muscle cell behaviour in cultured cells, and confocal microscopy to investigate their cytoskeleton. She collaborated with Graham Dunn to use Digitally Recorded Interference Microscopy with Automatic Phase Shifting (DRIMAPS) to investigate cell crawling behaviour. She moved to Leeds in 1997 as a Lecturer, and has continued to use a wide range of both light and electron microscopy approaches to investigate the molecular motors and the cytoskeleton.
Dr Susan Cox

King's College London
Susan works at the Randall Centre for Cell and Molecular Biophysics, developing fluorescence microscopy techniques and applying them to discover new cell biology at the nanoscale. In 2011 she was awarded a Royal Society University Research Fellowship, which she used to develop a substantial research program based around localisation microscopy, and methods to extract more information from super-resolution image data. SC is best known as the developer of Bayesian analysis of blinking and bleaching (3B), a method for analysing extremely dense localisation microscopy image series. Its importance has been recognised with the award of the Royal Microscopical Society light microscopy medal and the Society of Experimental Biology Presidents Medal. More recently, she has explored the limits of localisation in terms of speed and accuracy. She mathematically described the role of the size of the point spread function size in limiting information transmission speed and developed a machine learning based approach to remove poor fits from the super resolution image. Since it is obviously more desirable to avoid poor fits in the first place, she developed Haar Wavelet Kernel analysis (HAWK), an approach to localisation microscopy data analysis which avoids artifacts and ensures the results reflect the underlying structure of the sample.

Invited Speakers

Dr Jae-Byum Chang

MIT
Dr Siân Culley

King’s College London
Siân is a Royal Society University Research Fellow at King’s College London, working on developing new image analysis methods driven by real biological questions. Previously she was a postdoctoral research in Ricardo Henriques’ lab at the MRC Laboratory for Molecular Cell Biology, UCL, working on analytical methods for improving super-resolution fluorescence microscopy. Prior to joining Ricardo’s group, she did a PhD in Optical Microscopy and an undergraduate degree in Natural Sciences, both at UCL. In addition to research, Siân is also passionate about creating open source teaching material, outreach work, and driving towards better diversity and representation in the microscopy community.
Dr Alistair Curd

University of Leeds
Dr Ruth Hughes

University of Leeds
Prof Ralf Jungmann

Max Planck Institute of Biochemistry

Ralf Jungmann studied Physics at Saarland University, followed by a one-year diploma research stay with Paul Hansma at UC Santa Barbara where he worked on functional imaging of bone ultrastructure using Atomic Force Microscopy and High-Speed-Photography.
In 2007, Jungmann joined Prof. Friedrich C. Simmel’s lab at TU München as a Ph.D. student and was among the first researchers in Germany to apply and extend the DNA origami technique. During his Ph.D., Jungmann applied single-molecule fluorescence techniques to DNA Nanotechnology, constructing the first nanoscopic DNA origami “rulers” for super-resolution microscopy. He also pioneered a novel type of super-resolution microscopy, termed DNA-PAINT, that uses programmable DNA molecules as imaging probes.
After receiving his Ph.D. at TUM, he moved to the labs of Prof. Peng Yin and Prof. William M. Shih at the Wyss Institute for Biologically Inspired Engineering at Harvard University as an Alexander von Humboldt fellow. At Harvard, Jungmann worked on applications of DNA-PAINT for multiplexed cellular imaging.
In 2014, Jungmann received an Emmy Noether Fellowship from the German Research Foundation (DFG) and since then heads the research group “Molecular Imaging and Bionanotechnology” at the MPI of Biochemistry and the LMU Munich. In 2015, Jungmann co-founded Ultivue, a US-based company commercializing imaging reagents for DNA-PAINT super-resolution microscopy.
In 2016 he received an ERC Starting Grant to bring DNA-based super-resolution imaging from single molecules to whole cells and tissues. Since August 2016, he is Professor of Physics at the LMU Munich.
Dr Romain Laine

University of Cambridge
Romain studied Natural Sciences in at the ESPCI, Paris before moving to Imperial College London for a MSci in Optics and Photonics. He then carried on with an MRes in Chemical biology of health and disease and PhD at the Photonics group of Imperial specialising in advanced FLIM microscopy applied to cellular biology, with Prof. Paul French. He then moved to Cambridge to join Prof. Clemens Kaminski at the Laser Analytics Group and studies virus ultra-structure by applying state-of-the-art super-resolution microscopy and analytical methods.
Sandrine Leveque-Fort

Institut des Sciences Moléculaires d'Orsay
Sandrine Lévêque-Fort is a CNRS Researcher Director at the Institute of molecular science in Orsay (ISMO). She obtained her PhD on the development of a new acousto-optic imaging approach for imaging through scattering media in the Optical Lab of ESPCI. She then became a postdoctoral fellow in the physics department of Imperial College, where she started to develop time resolved fluorescence microscopy. She joined the CRNS in 2001, when she started to develop different strategies to improve fluorescence microscopy such as plasmonics. Since 2009, her research focus on supercritical angle fluorescence microscopy and super-resolution microscopy techniques.
Dr Victoria Lund

University of Sheffield
Dr Lothar Schermelleh

University of Oxford
2003 PhD at the Ludwig Maximilians University Munich (Advisor: Prof. Thomas Cremer)
2003-2011  Postdoctoral Researcher / Lecturer (Epigenetics & Bioimaging); Faculty of Biology, Ludwig Maximilians University Munich (Advisor: Prof. Heinrich Leonhardt).
2005-2007  Visiting Scientist with Prof. John W. Sedat, University of California, San Francisco.
Since 2011  Micron Senior Research Fellow / Principle Investigator at the Department of Biochemistry, University of Oxford
Prof Christian Soeller

University of Exeter

Prof. Christian Soeller obtained his PhD at the Max Planck Institute for Fluid Mechanics in Goettingen. He conducted postdoctoral research at the University of London where he developed and applied multi-photon microscopy to study the cell biology of cardiac contraction. He then took up a faculty position in the School of Medicine at the University of Auckland, New Zealand, where he continued his use of advanced microscopy techniques. As early adopters of super-resolution microscopy his group was able to see arrays of receptors in muscle cells with near molecular resolution for the first time. In 2012 he moved to the University of Exeter, UK, where he is a Professor of Physical Cell Biology in the interdisciplinary Living Systems Institute and continues his research into the use and advancement of super-resolution approaches to enable new biological insight.