Sponsored Content: Revealing life in its full context
Visualizing the dynamics of individual cells to understand the underlying mechanisms shaping complex tissues is an overarching goal in cell and developmental biology. However, visualizing single cells in the innermost layers of large 3D multicellular systems such as developing model organisms or 3D cell culture systems, including spheroids or organoids, is challenging. This is mainly due to light scattering causing poor penetration.
In addition, understanding complex processes in these samples often requires crossing large spatiotemporal biological scales. Due to its high spatiotemporal resolution and low phototoxicity light sheet microscopy should be the method of choice for imaging large living samples1.
Viventis Deep, from Leica Microsystems, is an open-top dual-view and dual-illumination light-sheet microscope purposefully designed for live imaging of large specimens at single-cell resolution. The configuration of objectives together with a customizable multiwell mounting system combines for the first-time to create a dual view light sheet imaging system with multiposition imaging2.
The unique combination of open top configuration, penetration depth into the sample easy sample mounting, throughput and long-term live imaging enables the gain of quantitative single-cell information in large specimens even over extended periods of time.
See it in action at mmc2025, booth #119
Join our TechnoBite Talk: Live imaging of large multicellular systems with open top dual view light sheet microscopy powered by Leica
Session: Live Imaging Across Scales: From Single Cells to Whole Organisms in Development and Disease.
Speaker: Tom Phillips, Advanced Workflow Specialist Lightsheet Microsopy, Leica Microsystems
Date and Time: Wednesday July 2 | 11:00
References:
[1] J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer. Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy. Science, vol. 305, no. 5686, Art. no. 5686, Aug. 2004, doi: 10.1126/science.1100035.
[2] Moos, F., Suppinger, S., de Medeiros, G. et al. Open-top multisample dual-view light-sheet microscope for live imaging of large multicellular systems. Nat Methods 21, 798–803 (2024).
https://doi.org/10.1038/s41592-024-02213-w