This workshop was organised by Prof Michelle Peckham and Dr Susan Cox and saw Prof Xiaowei Zhuang as one of the Speakers.

Designed to talk about the current challenges in developing and using super-resolution microscopy, this workshop encouraged discussion on topics including the potential for and feasibility of ‘home-built’ set-ups, and the types of analyses used to process and scrutinise the data. Thoughts and ideas were gathered to help define what is good/best practice around these challenging techniques, and give an insight into future potential developments.

The workshop included short practical demonstrations of iSIM, PALM/STORM and the Zeiss Airy Disc Instruments.

Programme

08:45    Registration

Analysis of Single Molecules/Super-Resolution Images

09.00    Ellipsoid Localisation Microscopy
09.15    Roundtable: How to establish a common file format for SR localization data
09.30    Fast nanoscopy with ImageJ through Super- Resolution Radial Fluctuations
09.45    Local dimensionality determines maximum localisation microscopy acquisition speed
10.00    Co-orientation: Quantifying simultaneous co- localization and orientational alignment of filaments in light microscopy
10.15    Sparse systems are no blinking good
10.30    Coffee Break
11.00    Illuminating biology at the nanoscale with Super-Resolution Imaging
12.00    Lunch, Demonstrations of iSIM, PALM/STORM and Airy Scan (Zeiss)
13.30    Correlative STED and AFM imaging of amyloid self-assembly reactions
13.45    Multicolour storm
14.00    iSIM
14.15    Design and characterization of a STED microscope for thick sample imaging
14.30    Interferometric scattering microscopy – from label- free detection of single molecules to Super- Resolution imaging without fluorescence
15.00    Coffee Break
15.30    Putting Super-Resolution to work
15.45    SPADs (Single Photon Avalanche Diodes)
16.00    Using Super-Resolution microscopy to identify human platelet granule disorders
16.15    Implementations of the Muller & Enderlein trick
16.30    General Discussion
17.00    Meeting Close