Visualising Bruton’s Tyrosine Kinase Inhibitors using Stimulated Raman Scattering Microscopy (infocus #73 March) 2024

Supported by an RMS Summer Studentship award, I spent time in the lab of Professor Duncan Graham at the University of Strathclyde to study the uptake and distribution of Bruton’s tyrosine kinase (Btk) inhibitors, ibrutinib, acalabrutinib and ibrutinib-yne using a combination of Raman and stimulated Raman scattering microscopy.

DOI: 10.22443/rms.inf.1.262

Raman imaging techniques are beginning to shed new insight into drug localisation and drugcell interactions without the use of exogenous tags and labels.

We sought to detect Btk inhibitors at their site of action in the cellular environment to detect differences in uptake concentration and localisation in a panel of cell lines expressing different levels of Btk. We also investigated the impact of Btk inhibition on cellular lipid metabolism.

Return to listing