Virtual Flow Cytometry Data Analysis Course Autumn 2021
13 – 16 September 2021
The RMS is holding an interactive virtual course on flow cytometric data analysis.
This course, which will run over 4 half-days, will cover the basics of analysis for a number of different applications including antibody phenotyping, DNA and cell cycle kinetics, cell proliferation and death, and functional studies. There will also be the opportunity to explore high-dimensional analysis using dimensionality reduction and clustering techniques.
Pre-recorded lectures will be provided in advance of the meeting with live small group practical sessions in the afternoons. It is run in the afternoon UK time to make it more accessible to those in other time zones (14:00 GMT/15:00 CET/09:00 EST).
Delegates will be able to use data files (which will be provided) and follow along with directed analysis by the course demonstrators. Analysis will be performed in either FlowJo or FCS Express and Applications specialists will also form part of the demonstrator team.
This course is open to all and is suitable for those who are relatively new to flow cytometry and who wish to expand their experience with applications and specific analysis.
Mr Steve Couzens
University of Wales College of Medicine
Steve is the current head of the Immunophenotyping laboratory in the Department of Haematology at the University Hospital of Wales in Cardiff. Having started as a Biomedical Scientist, Steve undertook the STP Equivalence process in 2014 and is now registered as a Clinical Scientist with the HCPC. He passed the RCPath Part 2 examination in 2015. Steve is a member of a number of expert groups including the Multi-Colour Immunophenotyping Group (MIG) and organises the clinical module of the annual RMS International Flow Cytometry Course.
The Francis Crick Institute
Derek runs a large flow cytometry core facility at Cancer Research UK’s London Research Institute which covers all aspects of cell analysis and sorting. He co-organises the section’s annual flow cytometry course at the University of York and is active in promotion of cytometry via focussed meetings and other courses. He is one of the principal organisers of the flowcytometryUK biennial meeting and also the Advances in Cytometry Meeting. Derek is particularly keen to promote cytometry education within the UK and beyond.
Dr Karen Hogg
Flow Cytometry Section Chair
University of York
Karen is currently a Senior Experimental Officer at the University of York, Bioscience Technology Facility. Karen underpins the scientific service within the Imaging and Cytometry Laboratory and takes a lead role in the research, operation and method development of cell sorting and analysis. As such, Karen’s expertise is utilized for a wide range of diverse applications both within and external to the Department of Biology. Karen is also a co-organizer and tutor on multiple flow cytometry courses throughout the UK. She got her BSc, Joint Honours in Biology and Biochemistry at Keele University, U.K. 1991-1995; then obtained her MSc in Applied Parasitology & Medical Entomology from the University of Liverpool, U.K. 1995-1996; then proceeded to obtain her Phd. from the School of Biology, University of Leeds, U.K. 1996-1999
Dr Peter O'Toole
RMS Vice President
University of York
Peter heads the Imaging and Cytometry Labs within the Technology Facility at the University of York which includes an array of confocal microscopes, flow cytometers and electron microscopes. Peter gained his PhD in the Cell Biophysics Laboratory at the University of Essex and has been involved in many aspects of fluorescence imaging. Research is currently focused on both technology and method development of novel probes and imaging modalities.
Peter has ongoing collaborations with many leading microscopy and cytometry companies and his group also provides research support to many academics and commercial organisations. Peter is also heavily involved with teaching microscopy and flow cytometry which includes organising and teaching on both the RMS Light Microscopy Summer School and the RMS Practical Flow Cytometry courses.
Francis Crick Institute