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- Flow Cytometry Course 2023
- Provisional Programme
Clinical Stream
Monday 4 September
09:00 – 09:30
Registration
Administration
×
Registration
09:00 – 09:30 BST, 4 September 2023 ‐ 30 mins
Administration
09:30 – 10:00
Fluorochromes and Fluorescence
Lecture
×
Fluorochromes and Fluorescence
09:30 – 10:00 BST, 4 September 2023 ‐ 30 mins
Lecture
10:00 – 10:25
Antibody Labelling and Immunofluorescence
Lecture
×
Antibody Labelling and Immunofluorescence
10:00 – 10:25 BST, 4 September 2023 ‐ 25 mins
Lecture
10:55 – 11:40
Introduction to the Flow Cytometer: Fluidics, Optics and Lasers
Lecture
×
Introduction to the Flow Cytometer: Fluidics, Optics and Lasers
10:55 – 11:40 BST, 4 September 2023 ‐ 45 mins
Lecture
11:40 – 12:30
Detectors and Signal Processing
Lecture
×
Detectors and Signal Processing
11:40 – 12:30 BST, 4 September 2023 ‐ 50 mins
Lecture
12:30 – 12:50
System Walk-Around
Practical
×
System Walk-Around
12:30 – 12:50 BST, 4 September 2023 ‐ 20 mins
Practical
13:30 – 14:30
The Flow Cytometers (including routine checks of performance - amplifier linearity, sensitivity)
Practical
×
The Flow Cytometers (including routine checks of performance - amplifier linearity, sensitivity)
13:30 – 14:30 BST, 4 September 2023 ‐ 1 hour
Practical
14:30 – 15:00
Quality Control in Flow Cytometry using Commercially Available Beads (8-peak beads and other multipeak/colour)
Practical
×
Quality Control in Flow Cytometry using Commercially Available Beads (8-peak beads and other multipeak/colour)
14:30 – 15:00 BST, 4 September 2023 ‐ 30 mins
Practical
15:30 – 16:30
Multicolour Design and Compensation
Lecture
×
Multicolour Design and Compensation
15:30 – 16:30 BST, 4 September 2023 ‐ 1 hour
Lecture
Tuesday 5 September
09:15 – 10:45
Multiparamater Labelling - Fluorescence Compensation (peripheral blood lymphocytes - colour compensation)
Practical
×
Multiparamater Labelling - Fluorescence Compensation (peripheral blood lymphocytes - colour compensation)
09:15 – 10:45 BST, 5 September 2023 ‐ 1 hour 30 mins
Practical
11:15 – 13:00
Practical Continued
Practical
×
Practical Continued
11:15 – 13:00 BST, 5 September 2023 ‐ 1 hour 45 mins
Practical
13:45 – 15:15
Linear Analysis and Doublet Discrimination - Using Cell Cycle as Examples
Practical
×
Linear Analysis and Doublet Discrimination - Using Cell Cycle as Examples
13:45 – 15:15 BST, 5 September 2023 ‐ 1 hour 30 mins
Practical
Wednesday 6 September
09:00 – 09:15
Registration for Advanced Course Attendees
Administration
×
Registration for Advanced Course Attendees
09:00 – 09:15 BST, 6 September 2023 ‐ 15 mins
Administration
09:15 – 10:15
Clinical Assay Design, Guidelines and Best Practice
Lecture
×
Clinical Assay Design, Guidelines and Best Practice
09:15 – 10:15 BST, 6 September 2023 ‐ 1 hour
Lecture
10:15 – 10:45
Quality Assurance
Lecture
×
Quality Assurance
10:15 – 10:45 BST, 6 September 2023 ‐ 30 mins
Lecture
10:45 – 11:15
Tea/Coffee and Trade Exhibition
Break
×
Tea/Coffee and Trade Exhibition
10:45 – 11:15 BST, 6 September 2023 ‐ 30 mins
Break
11:15 – 11:45
Quality Assurance
Lecture
×
Quality Assurance
11:15 – 11:45 BST, 6 September 2023 ‐ 30 mins
Lecture
11:45 – 12:15
CD4 Counting
Lecture
×
CD4 Counting
11:45 – 12:15 BST, 6 September 2023 ‐ 30 mins
Lecture
12:15 – 12:45
Immunodeficiency
Lecture
×
Immunodeficiency
12:15 – 12:45 BST, 6 September 2023 ‐ 30 mins
Lecture
12:45 – 13:45
Lunch and Trade Exhibition
Break
×
Lunch and Trade Exhibition
12:45 – 13:45 BST, 6 September 2023 ‐ 1 hour
Break
13:45 – 15:00
PNH/FMH
Lecture
×
PNH/FMH
13:45 – 15:00 BST, 6 September 2023 ‐ 1 hour 15 mins
Lecture
15:00 – 15:45
Myeloma/Plasma Cells
Lecture
×
Myeloma/Plasma Cells
15:00 – 15:45 BST, 6 September 2023 ‐ 45 mins
Lecture
15:45 – 16:15
Tea/Coffee and Trade Exhibition
Break
×
Tea/Coffee and Trade Exhibition
15:45 – 16:15 BST, 6 September 2023 ‐ 30 mins
Break
Thursday 7 September
10:00 – 10:45
Rare Event Analysis
Lecture
×
Rare Event Analysis
10:00 – 10:45 BST, 7 September 2023 ‐ 45 mins
Lecture
11:15 – 12:00
Acute Lymphoblastic Leukaemia
Lecture
×
Acute Lymphoblastic Leukaemia
11:15 – 12:00 BST, 7 September 2023 ‐ 45 mins
Lecture
12:00 – 12:45
Acute and Chronic Myeloid Disorders
Lecture
×
Acute and Chronic Myeloid Disorders
12:00 – 12:45 BST, 7 September 2023 ‐ 45 mins
Lecture
13:45 – 14:30
Lymphoproliferative Disease
Lecture
×
Lymphoproliferative Disease
13:45 – 14:30 BST, 7 September 2023 ‐ 45 mins
Lecture
14:30 – 15:15
Case Studies: Leukaemia and Lymphoma Integrated Reporting
Discussion
×
Case Studies: Leukaemia and Lymphoma Integrated Reporting
14:30 – 15:15 BST, 7 September 2023 ‐ 45 mins
Discussion
16:15 – 16:45
Rare Event Analysis
Practical
×
Rare Event Analysis
16:15 – 16:45 BST, 7 September 2023 ‐ 30 mins
Practical
Friday 8 September
09:30 – 11:00
Data Analysis & Reporting
Workshop
×
Data Analysis & Reporting
09:30 – 11:00 BST, 8 September 2023 ‐ 1 hour 30 mins
Workshop
11:30 – 12:00
Data Presentation Guidelines
Lecture
×
Data Presentation Guidelines
11:30 – 12:00 BST, 8 September 2023 ‐ 30 mins
Lecture
12:00 – 12:45
Spectral Flow Cytometry
Lecture
×
Spectral Flow Cytometry
12:00 – 12:45 BST, 8 September 2023 ‐ 45 mins
Lecture
13:30 – 14:00
Other Applications of Flow Cytometry
Lecture
×
Other Applications of Flow Cytometry
13:30 – 14:00 BST, 8 September 2023 ‐ 30 mins
Lecture
Research Stream
Monday 4 September
09:00 – 09:30
Registration
Administration
×
Registration
09:00 – 09:30 BST, 4 September 2023 ‐ 30 mins
Administration
09:30 – 10:00
Fluorochromes and Fluorescence
Lecture
×
Fluorochromes and Fluorescence
09:30 – 10:00 BST, 4 September 2023 ‐ 30 mins
Lecture
10:00 – 10:25
Antibody Labelling and Immunofluorescence
Lecture
×
Antibody Labelling and Immunofluorescence
10:00 – 10:25 BST, 4 September 2023 ‐ 25 mins
Lecture
10:55 – 11:40
Introduction to the Flow Cytometer: Fluidics, Optics and Lasers
Lecture
×
Introduction to the Flow Cytometer: Fluidics, Optics and Lasers
10:55 – 11:40 BST, 4 September 2023 ‐ 45 mins
Lecture
11:40 – 12:30
Detectors and Signal Processing
Lecture
×
Detectors and Signal Processing
11:40 – 12:30 BST, 4 September 2023 ‐ 50 mins
Lecture
12:30 – 12:50
System Walk-Around
Practical
×
System Walk-Around
12:30 – 12:50 BST, 4 September 2023 ‐ 20 mins
Practical
13:30 – 14:30
The Flow Cytometers (including routine checks of performance - amplifier linearity, sensitivity)
Practical
×
The Flow Cytometers (including routine checks of performance - amplifier linearity, sensitivity)
13:30 – 14:30 BST, 4 September 2023 ‐ 1 hour
Practical
14:30 – 15:00
Quality Control in Flow Cytometry using Commercially Available Beads (8-peak beads and other multipeak/colour)
Practical
×
Quality Control in Flow Cytometry using Commercially Available Beads (8-peak beads and other multipeak/colour)
14:30 – 15:00 BST, 4 September 2023 ‐ 30 mins
Practical
15:30 – 16:30
Multicolour Design and Compensation
Lecture
×
Multicolour Design and Compensation
15:30 – 16:30 BST, 4 September 2023 ‐ 1 hour
Lecture
Tuesday 5 September
09:15 – 10:45
Multiparamater Labelling - Fluorescence Compensation (peripheral blood lymphocytes - colour compensation)
Practical
×
Multiparamater Labelling - Fluorescence Compensation (peripheral blood lymphocytes - colour compensation)
09:15 – 10:45 BST, 5 September 2023 ‐ 1 hour 30 mins
Practical
11:15 – 13:00
Practical Continued
Practical
×
Practical Continued
11:15 – 13:00 BST, 5 September 2023 ‐ 1 hour 45 mins
Practical
13:45 – 15:15
Linear Analysis and Doublet Discrimination - Using Cell Cycle as Examples
Practical
×
Linear Analysis and Doublet Discrimination - Using Cell Cycle as Examples
13:45 – 15:15 BST, 5 September 2023 ‐ 1 hour 30 mins
Practical
Wednesday 6 September
09:00 – 09:15
Registration for Advanced Course Attendees
Administration
×
Registration for Advanced Course Attendees
09:00 – 09:15 BST, 6 September 2023 ‐ 15 mins
Administration
09:15 – 10:00
Analysis of Micro-Organisms and Sub-micron Particles
Lecture
×
Analysis of Micro-Organisms and Sub-micron Particles
09:15 – 10:00 BST, 6 September 2023 ‐ 45 mins
Lecture
10:00 – 10:45
Receptor Quantification
Lecture
×
Receptor Quantification
10:00 – 10:45 BST, 6 September 2023 ‐ 45 mins
Lecture
10:45 – 11:15
Tea/Coffee and Trade Exhibition
Break
×
Tea/Coffee and Trade Exhibition
10:45 – 11:15 BST, 6 September 2023 ‐ 30 mins
Break
11:15 – 11:55
Intracellular Staining (including fixation and permeabilisation)
Lecture
×
Intracellular Staining (including fixation and permeabilisation)
11:15 – 11:55 BST, 6 September 2023 ‐ 40 mins
Lecture
11:55 – 13:15
Studying Bacteria
Practical
×
Studying Bacteria
11:55 – 13:15 BST, 6 September 2023 ‐ 1 hour 20 mins
Practical
13:15 – 14:15
Lunch and Trade Exhibition
Break
×
Lunch and Trade Exhibition
13:15 – 14:15 BST, 6 September 2023 ‐ 1 hour
Break
14:15 – 14:45
Receptor Quantification
Practical
×
Receptor Quantification
14:15 – 14:45 BST, 6 September 2023 ‐ 30 mins
Practical
14:45 – 15:45
Proliferation
Lecture
×
Proliferation
14:45 – 15:45 BST, 6 September 2023 ‐ 1 hour
Lecture
15:45 – 16:15
Tea/Coffee and Trade Exhibition
Break
×
Tea/Coffee and Trade Exhibition
15:45 – 16:15 BST, 6 September 2023 ‐ 30 mins
Break
Thursday 7 September
10:00 – 10:45
Studying Apoptosis by Flow Cytometry
Lecture
×
Studying Apoptosis by Flow Cytometry
10:00 – 10:45 BST, 7 September 2023 ‐ 45 mins
Lecture
11:15 – 11:45
Measurement of Cell Proliferation: The Dye Dilution Method
Lecture
×
Measurement of Cell Proliferation: The Dye Dilution Method
11:15 – 11:45 BST, 7 September 2023 ‐ 30 mins
Lecture
11:45 – 12:15
Measurement of Cell Cycle Parameters using BrdUrd/Anti BrUrd
Practical
×
Measurement of Cell Cycle Parameters using BrdUrd/Anti BrUrd
11:45 – 12:15 BST, 7 September 2023 ‐ 30 mins
Practical
12:15 – 12:45
Cell Proliferation by Dye Dilution
Practical
×
Cell Proliferation by Dye Dilution
12:15 – 12:45 BST, 7 September 2023 ‐ 30 mins
Practical
13:45 – 14:15
Antibody Titration
Practical
×
Antibody Titration
13:45 – 14:15 BST, 7 September 2023 ‐ 30 mins
Practical
14:15 – 15:15
Apoptosis: a/ Live/Dead, b) Sub-G1 Peak, c) Annexin V Labelling, d) Dye Uptake, e) Mitochondrial Membrane Potential
Practical
×
Apoptosis: a/ Live/Dead, b) Sub-G1 Peak, c) Annexin V Labelling, d) Dye Uptake, e) Mitochondrial Membrane Potential
14:15 – 15:15 BST, 7 September 2023 ‐ 1 hour
Practical
15:45 – 16:45
Fluorescent Proteins, FRET and Flow Cytometry
Lecture
×
Fluorescent Proteins, FRET and Flow Cytometry
15:45 – 16:45 BST, 7 September 2023 ‐ 1 hour
Lecture
Friday 8 September
09:30 – 10:00
Imaging Flow Cytometry
Lecture
×
Imaging Flow Cytometry
09:30 – 10:00 BST, 8 September 2023 ‐ 30 mins
Lecture
10:00 – 11:00
High Speed Cell Sorting
Practical
×
High Speed Cell Sorting
10:00 – 11:00 BST, 8 September 2023 ‐ 1 hour
Practical
11:30 – 12:00
Data Presentation Guidelines
Lecture
×
Data Presentation Guidelines
11:30 – 12:00 BST, 8 September 2023 ‐ 30 mins
Lecture
12:00 – 12:45
Spectral Flow Cytometry
Lecture
×
Spectral Flow Cytometry
12:00 – 12:45 BST, 8 September 2023 ‐ 45 mins
Lecture
13:30 – 14:00
Other Applications of Flow Cytometry
Lecture
×
Other Applications of Flow Cytometry
13:30 – 14:00 BST, 8 September 2023 ‐ 30 mins
Lecture