Super-resolution Workshop 2023

Joerg Bewersdorf

Yale University, USA

Joerg Bewersdorf is a Professor of Cell Biology and of Biomedical Engineering at Yale University.  He received his Master's degree (Dipl Phys, 1998) and his doctoral degree in physics (Dr rer nat, 2002) training with Dr Stefan W Hell at the Max Planck Institute for Biophysical Chemistry in Goettingen, Germany.  After 4 years at The Jackson Laboratory in Bar Harbor, Maine, he relocated his research group to Yale University in 2009.  An optical physicist/biophysicist by training, Dr Bewersdorf has been a long-time contributor to the field of super-resolution light microscopy development and the application of these techniques to cell biological questions.

 

Christian Eggeling

Leibniz Institute of Photonic Technologies, Germany

Dr. Eggeling holds a PhD in Physics from the University of Göttingen, Germany, where he optimized single-molecule fluorescence detection. From 2000 to 2003 he was a research scientist at the biotech company Evotec, Hamburg, Germany, developing advanced fluorescence microscopy techniques for high-throughput drug screening. In 2003, Christian Eggeling joined the Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany as a senior scientist in the department of Professor Stefan Hell, the 2014 Nobel Laureate in Chemistr). Here, he was focused on the field of optical super-resolution microscopy, specifically the biological applicability of stimulated emission depletion (STED) microscopy. 
Since 2012, Christian Eggeling has been a principal investigator in the Human Immunology Unit and the scientific director of the newly established Wolfson Imaging Centre Oxford at the Weatherall Institute of Molecular Medicine, University of Oxford, United Kingdom, and in 2014 he has been appointed Professor of Molecular Immunology. From December 2017 on he in addition started as a Professor of Super-Resolution Microscopy and director of the Institute of Applied Optics and Biophysics (IAOB) at the Friedrich Schiller-University Jena, and as the Head of the Department of Biophysical Imaging at the Leibniz Institute of Photonic Technologies in Jena, Germany. Christian Eggeling’s research is focused on the development of advanced microscopy for the investigation of molecular organization and dynamics in cells, especially on the cellular plasma membrane, e.g. after infection or immune responses.

Juliette Griffié

Stockholm University, Sweden

In 2023, Juliette Griffié will start her research group: the Computational Microscopy for Cell Biology lab (https://www.scilifelab.se/researchers/juliette-griffie/) at SciLifeLab in Stockholm, as a DDLS fellow and assistant Prof. for the department of Biochemistry and Biophysics at the University of Stockholm. She holds a dual Master’s degree (2013), with majors in Engineering and Physics, and a PhD degree in Biophysics (King’s College London, Prof. D. Owen’s laboratory, 2017). Prior to her current position, she was awarded an MSCA post-doctoral fellowship in 2020 at EPFL (Switzerland). Her research interests span from advanced light microscopy techniques to data driven analysis tools aimed at answering fundamental biological issues. The CMCB lab will focus on developing cutting edge technological solutions to better understand immune cells activation.

Sandrine Leveque-Fort

Paris Saclay University, France

She obtained her PhD on the development of a new acousto-optic imaging approach for imaging through scattering media in the Optical Lab of ESPCI in Paris. She then became a postdoctoral fellow at Imperial College, where she started to develop time resolved fluorescence microscopy but also structured illumination strategy. She joined the CNRS in 2001 to develop different strategies to improve spatial and temporal resolution for fluorescence microscopy, by implementing new configurations for FLIM-FRET imaging or by introducing plasmonics substrates to engineer fluorescence emission.  Since 2009, she has proposed various approaches to take advantage of supercritical angle fluorescence (SAF) emission as an alternative intrinsic tool given by the fluorophore itself to access axial information. Since 2012, her research is focused in super-resolution microscopy and in particular she developed  new approaches in single molecule localization microscopy to reveal quantitatively the 3D cellular nano-organization. Since 2016, she introduced a time signature within the localization process, this technique called ModLoc permits to retrieve the fluorophores’ information thanks to the phase of their modulated emission and benefits of an enhanced localization. This approach opens in depth 3D imaging in complex biological samples such as embryos or tissues.  Her current developments aimed at offering both structural and functional information at nanoscale in living cells. 

Lothar Schermelleh

University of Oxford, UK

Dr Lothar Schermelleh studied Biology at the Ludwig Maximilian University Munich, where he obtained his doctorate under the mentorship of Thomas Cremer in 2003. During this time, he established bespoke chromatin labelling and 4D imaging approaches to analyse cell cycle-dependent chromosomal dynamics.
He later joined the group of Heinrich Leonhardt, where he investigated the role and regulation of a key epigenetic factor DNA methyltransferase 1 (Dnmt1), using bespoke quantitative single-cell imaging and FRAP analysis pipelines. During a research stay in the lab of John Sedat (UCSF), he became the first to successfully apply super-resolution 3D structured illumination microscopy (3D-SIM) to study complex biological structures, namely the three-dimensional organisation of the nuclear periphery, published in a landmark 2008 Science paper.
In 2011, Lothar took up a Senior Research Fellow position at the University of Oxford, where his group further advanced super-resolution 3D imaging to study mammalian chromatin domain organisation in the context of epigenetic gene regulation. He also contributed to the discovery of de novo chromatin domain formation required for DNA double-strand break repair, and to elucidate molecular processes underpinning X-chromosome inactivation. Besides, his group developed protocols and computational analysis tools for standardised quality control of super-resolution SIM data.
In 2020, Lothar was named Associate Professor and became Academic Director of the Micron Bioimaging Facility. His publications have been cited >12,000 times (h-index 49; Google Scholar, Feb 2023), demonstrating his significant contribution to the development and cell biological application of advanced microscopy methods.

Hari Shroff

Janelia Research Campus, USA

Dr. Hari Shroff received a B.S.E. in bioengineering from the University of Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed his Ph.D. in biophysics at the University of California at Berkeley in 2006. He spent the next three years performing postdoctoral research in Eric Betzig’s lab at the Howard Hughes Medical Institute's Janelia Research Campus where his research focused on the development of photoactivated localization microscopy (PALM), an optical super-resolution technique that earned Betzig a share of the 2014 Nobel Prize in Chemistry. For the next thirteen years, Shroff headed the Laboratory of High Resolution Optical Imaging at the National Institute of Biomedical Imaging and Bioengineering at the National Institutes of Health, working to improve fluorescence microscopy methods suited for volumetric time-lapse (‘4D’) imaging. Commercialized inventions from this period include dual-view selective plane illumination microscopy (diSPIM) and instant super-resolution microscopy (iSIM). Shroff recently returned to Janelia, where he continues to push the envelope in biological imaging. Current areas of research include adaptive optics, deep learning, and the study of brain development in simple organisms.