Mechanical Meniscus Correction in Phase Contrast Microscopy (infocus #73 March 2024)

Imaging living cell samples is challenging due to the meniscus formed by immersing liquid at container walls.

DOI: 10.22443/rms.inf.1.265

The meniscus distorts the passage of illumination, creating artefacts in
transmitted light microscopy. This is especially a problem with phase contrast microscopy, where the illuminating annulus must match the phase ring to produce phase contrast (as in Figure 1).

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