All timings are Grenwich Mean Time (GMT)

Please note this programme is provisional and may change.

Monday 16 March
09:30 – 10:00
Registration for in-person (Hybrid) delegates

Administration

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10:00 – 10:15
Welcome & Virtual Tour of the nmRC

Administration

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10:15 – 11:00
Sample Preparation for Cryo-Microscopy

Theory

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11:00 – 11:30
Cryo-Scanning Electron Microscopy

Theory

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11:30 – 12:15
Cryo-Transmission Electron Microscopy

Theory

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12:15 – 12:30
Lunch

Break

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12:30 – 12:40
Techno Bite - Linkam Scientific

Lunch/Techno Bite

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12:40 – 12:50
Techno Bite - AMETEK

Lunch/Techno Bite

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12:50 – 13:00
Techno Bite - Quorum

Lunch/Techno Bite

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13:00 – 13:10
Explanation of sessions & groups

Administration

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13:10 – 14:55
High Pressure Freezing, Plunge Freezing & AutoGrid Clipping

Demonstration

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14:55 – 15:15
Break

Break

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15:15 – 17:00
Metal Mirror/Cryo-SEM, Slush Freezing/Cryo-SEM, Cryo-TEM Software & Cryo-TEM Holders/Loading

Demonstration

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Tuesday 17 March
09:45 – 10:00
Review of Day One

Administration

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10:00 – 10:25
Cryo Light Microscopy

Theory

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10:25 – 11:00
Correlative Microscopy (under Cryogenic Conditions)

Theory

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11:00 – 11:40
Cryo-Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)

Theory

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11:40 – 12:15
Cryo Secondary Ion Mass Spectrometry (SIMS)

Theory

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12:15 – 12:30
Lunch

Break

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12:30 – 12:40
Techno Bite - Carl Zeiss

Lunch/Techno Bite

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12:40 – 12:50
Techno Bite - JEOL

Lunch/Techno Bite

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12:50 – 13:00
Techno Bite - Thermo Fisher

Lunch/Techno Bite

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13:00 – 13:10
Description of sessions

Administration

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13:10 – 14:55
Cryogenic Light Microscopy, Correlative Microscopy, Cryo FIB-SEM, Cryo-TEM

Demonstration

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14:55 – 15:00
Break

Break

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15:00 – 16:00
VCT & VCM Transfer, OrbiSIMS, Advanced Cryo-SEM

Demonstration

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16:00 – 17:00
Cryogenic Light Microscopy, Correlative Microscopy, Cryo-TEM, Cryo-FIB

Demonstration

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Wednesday 18 March
09:45 – 10:00
Review of Day Two

Administration

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10:00 – 10:35
Cryo Soft X-Ray Microscopy

Theory

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10:35 – 11:20
Putting it all Together - Overview of Modern Cryo-Microscopy

Theory

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11:20 – 12:00
Artefacts in CryoEM & Next Steps

Theory

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12:00 – 13:00
Lunch

Break

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13:00 – 14:30
Ultramicrotomy, Plunge Freezing to Cryo-TEM/SEM, Cryo-LM to Cryo-FIB

Demonstration

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14:30 – 14:45
Coffee & Certificates

Break

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14:45 – 16:15
Ultramicrotomy, Plunge Freezing to Cryo-TEM/SEM, Cryo-LM to Cryo-FIB

Demonstration

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16:15 – 16:30
Farewell

Administration

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