All Things Cryo - a virtual course
21 – 24 September 2021
There can be no doubt that Cryo-EM continues to be a hot topic following the 2017 Nobel Prize in Chemistry for its development. In fact many cryo-techniques have been around for over 40 years and are in use in both academic and industrial settings being applied to a full range of soft matter and materials samples beyond single particle analysis of proteins. This course looks to educate scientists and engineers to the full range of cryo-techniques, who are looking to characterise, analyse and understand structure / function of their samples where stabilisation of volatiles (often water) is required. Topics covered are expected to include:
- Sample preparation / stabilisation (high-pressure freezing / plunge freezing)
- Freeze-substitution / ultramicrotomy
- Cryo-fluorescence microscopy / correlative microscopy
- Cryo-SEM / Cryo-FIB-SEM
The course will be delivered by an international group of speakers, staff from the NMRC, Nottingham and representatives from many commercial partners. The course is a mix of both lecture style presentations and remote demonstration practical sessions, with plenty of opportunities to ask questions and explore the range of cryo-techniques on offer. By the end of the week attendees will have a good understanding of cryo-principles and appropriate usage of cryo-techniques as well as developing a network of contacts for the future.
We look forward to welcoming you (virtually!) to Nottingham.
Dr Chris Parmenter
University of Nottingham
Christopher is a Research Officer in Cryogenic Electron Microscopy. His PhD is in polymer chemistry through which he developed skills in Cryo-TEM whilst investigating soft and biological systems. He recently moved into SEM, specifically Cryo-SEM and FIB-SEM of any samples where there is a nano-scale problem to solve or understand. He is keen to meet with other microscopists to further his knowledge of EM & other techniques.
Chris is currently the Editor of Microscopy & Analysis.