Imaging ONEWORLD - 'Horses for the courses: strategic adaptation of super-resolution microscopy modalities to solve the structural basis of cell signalling' - Dr Izzy Jayasinghe
7 June 2021
This week will feature Dr Izzy Jayasinghe, The University of Sheffield
Scientific Organisers: Stefanie Reichelt, Alex Sossick, Nick Barry, Alessandro Esposito and Kirti Prakash
The meeting will begin at 1pm BST.
As part of the 'Imaging ONEWORLD' series, the focus of these lectures is on microscopy and image analysis methods and how to apply these to your research. Almost all aspects of imaging such as sample preparation, labelling strategies, experimental workflows, ‘how-to’ image and analyse, as well as facilitating collaborations and inspiring new scientific ideas will be covered. Speakers will be available for questions and answers. The organisers, CRUK CI core facility staff, Gurdon Institute, MRC-LMB, MRC Cancer Unit and NPL will be able to continue the discussion and provide advice on your imaging projects.
Dr Izzy Jayasinghe
Izzy Jayasinghe is a Senior Research Fellow and a UKRI Future Leader Fellow in the Department of Molecular Biology & Biotechnology in the University of Sheffield. Her research has focused on developing new optical microscopy techniques for studying the organisation of the molecules of life, particularly proteins, within the heart. Prior to moving to Sheffield, Izzy completed a PhD in Physiology in Auckland (New Zealand) and two postdoctoral fellowships in Queensland (Australia) and Exeter where she established a track record in developing and applying new optical imaging methods. She established her independent research group in the University of Leeds in 2015 where developed adaptations of optical imaging methods such as DNA-PAINT and Expansion microscopy to study pathological nanoscale remodelling in the failing heart. Her current research focuses on developing more accessible, faster and higher resolution imaging methods for imaging optically-thick (and biologically more complex) samples.
In this seminar, I will outline how the continued improvement and strategic adaptation of super-resolution microscopy tools have enhanced our understanding of the nanoscale structures underpinning life. I will illustrate some of these advances with examples where super-resolution modalities have allowed us to better-visualise ryanodine receptor (RyR) nanodomains in tissues like the heart, muscle and neural tissues. Correlative structure-function imaging has allowed us to resolve, observe and identify the RyR clustering patterns which spatially-encode the fast calcium signals within the cells. I will also introduce some of the newer modalities of localisation microscopy that we are developing, such as sandSTORM, which offer a useful workaround some of the key bottlenecks in super-resolution implementation at present.