Scientific Organisers: Stefanie Reichelt, Alex Sossick, Nick Barry, Alessandro Esposito and Kirti Prakash
The meeting will begin at 1pm BST.
As part of the 'Imaging ONEWORLD' series, the focus of these lectures is on microscopy and image analysis methods and how to apply these to your research. Almost all aspects of imaging such as sample preparation, labelling strategies, experimental workflows, ‘how-to’ image and analyse, as well as facilitating collaborations and inspiring new scientific ideas will be covered. Speakers will be available for questions and answers. The organisers, CRUK CI core facility staff, Gurdon Institute, MRC-LMB, MRC Cancer Unit and NPL will be able to continue the discussion and provide advice on your imaging projects.
Speaker
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Dr Chaitanya Athale
Chaitanya Athale studied M.Sc. Zoology at Pune University in India in 1998. He went on to do a PhD at the University of Heidelberg, Germany with Dr. Roland Eils in modelling and measuring intracellular diffusion in 2003. His first postdoc involved agent based modeling tumour growth at the Massachusetts General Hospital in Boston. For his second postdoc he worked with Eric Karsenti on microtubule regulation by spatial gradients combining experiment and theory. Since 2009 he has been working in IISER Pune, looking a the role of cytoskeleton in cell shape, by a combination of computer simulations, in vitro reconstitution and microscopy and image analysis.
Speaker's Abstract
Label free imaging has undergone further revitalisation with improvements in algorithms and computing power. Combined with fluorescence tagging of molecules the method offers an approach to connect genotype with phenotype and answer long standing questions in cell biology. In this talk I will discuss two aspects of some work we have lately been doing on the detection and measurement of cell size in differential interference contrast (DIC) images and following intracellular flows by combining a filtering and single particle tracking approach. We discuss how the use of fixed images of Escherichia coli cells in DIC counterstained with molecular markers helped us address some of the classic questions in bacterial cell division about the role of replication-division coupling in cell size determination. We also use the movement of high-contrast intracellular lipid granules in single celled embryos of Caenorhabditis elegans to estimate flows due to spindle oscillations. We conclude with how we see these low level methods of DIC structure and shape detection feeding into future developments using learning methods for better quantitative and non-invasive cellular imaging.