Advanced Light Microscopy Core (ALM)

The Advanced Light Microscopy Core at Penn State College of Medicine offers consultation and training for fluorescence imaging and bioimage analysis. Our confocal, super-resolution (STED), light-sheet, and widefield microscopes allow for imaging samples at multiple scales. Image analysis workstations with Imaris and Huygens are excellent tools for image processing, analysis, and visualization. Non-Penn State users are welcome!



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Expertise

Imaging Platforms 

keywords - Light microscopy: Superresolution microscopy, Live cell imaging, Gated-STED, Light-sheet microscopy (SPIM), Confocal microscopy, Widefield microscopy, CW-STED, FCS/FCCS

Laser scanning confocal microscopes include the Leica SP8 FALCON (with FLIM/FCS) and Leica SP8 STED 3X (super-resolution with three depletion lasers).

Light-sheet imaging of large cleared samples (tissue, organs, embryos, etc.) is performed with an UltraMicroscope II.

Widefield microscopy is provided by a DeltaVision Elite.

Applications 

Keywords - biological: Cell Biology, Developmental Biology, C.elegans, Microbiology, Neurobiology, Immunobiology, Drosophila

 

Sample Preparation

Keywords - biological: Immunofluorescence, Immunolabelling, Live cell

We can provide guidance on sample preparation, but we do not offer sample preparation as a service.

 

Data Analysis

keywords - software: ImageJ, Imaris, Huygens, Fiji

The latest analysis workstation is equipped with a 24-core CPU, 512GB of RAM, an NVIDIA 4090 GPU, and 8TB of NVMe storage. It is capable of handling large datasets with licenses for Imaris and Huygens Professional. Other software includes Fiji, ilastik, Qupath, and Napari.



Point of Contact

Advanced Light Microscopy Core (ALM)
500 University Drive
Hershey , PA 17033, USA


 

William Giang

Email: advancedLightMicroscopy@pennstatehealth.psu.edu

Visit the Advanced Light Microscopy Core (ALM) website