Expertise
Imaging Platforms
Keywords - Light Microscopy: Superresolution microscopy | Live cell imaging | Gated-STED | TCSPC-FLIM | Light-sheet microscopy (SPIM) | Frequency-domain FLIM | Confocal microscopy | Widefield microscopy | TIRF | Multi-photon microscopy | CW-STED | FCS/FCCS
The Facility contains over 20 optical microscopes including: Point Scanning, Spinning Disc and Two Photon Confocals, Lattice Lightsheet, SPIM, OPT, and Automated Widefield systems.
Including:
- 3 x Zeiss 710 incl. Airyscan, 2photon and GaAsP detectors
- Zeiss 880 Fast Airyscan
- Leica SP8 STED3X incl. Falcon/ Lightnight/Navigator/Resonant
- Lattice Lightsheet
- Andor Dragonfly Spinning Disc
- Zeiss Spinning Disc
- Multiple Automated Widefield Microscopes
Applications
Keywords – Biological: Cell Biology | Developmental Biology | Plant Biology | Immunobiology | Zebrafish
Sample Preparation
Keywords - Biological: Immunofluorescence | Live cell
Data Analysis
Keywords - Software: ImageJ | Imaris | Huygens | Fiji | Amira | AutoQuant
Analysis virtual machines are high-powered, graphics accelerated computers available to all users of the imaging facility to access, either onsite at the Queensland Bioscience Precinct, or remotely via VPN. These machines are installed with multiple different software packages including all of the relevant vender software (Zen, LAS X, NIS Elements Viewer) as well as advanced visualisation and analysis tools including Imaris 9, and FIJI/ImageJ.
These virtual machines are Big Data Ready! This means they have a large allocation of RAM (1Tb or more), 3 x Volta V100 16Gb GPU processors each, high-speed network connections, a large dedicated scratch workspace (200Tb) and the latest visualisation software, including Imaris 9 and Arivis-4D. For large datasets (Spinning Disc Confocal, Lattice Lightsheet Microscope, Nikon deconvolution) we recommend Imaris 9 for visualisation and quantification.
Funding
Many of the systems within the facility have been funded by the Australian Cancer Research Foundation, Australian Research Council, National Health and Medical Research Council and the University of Queensland