Faculty of Medicine and Dentistry University of Plymouth

Our researcher’s interests focus on brain cancers, immunology, neurobiology, neurodegeneration, genomics, microbiology, hepatology, haematology and stem cell research. We therefore have a broad expertise in all relevant fields of translational biomedical sciences. To meet the light microscopy needs of our staff, the School invested in a modern Imaging Suite which allows fast documentation of live cell cultures, in situ conventional and fluorescent stains and quantitative image analysis.



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Expertise

Imaging Platforms

Keywords - Light Microscopy: Laser microdissection | Live cell imaging | Confocal microscopy | Widefield microscopy
Keywords - Medical Imaging: in vivo fluorescence imaging 

At the heart of our Imaging Suite are three confocal microscopes a Leica SP8 equipped with three gas lasers, one LED, five detectors (including one HyD) and an environment chamber allowing control of gas composition and temperature for live imaging. A second Leica SPE confocal equipped with three solid state lasers, two LEDs and two detectors which is setup for fast scanning using a galvo stage. A third confocal a Zeiss 510/ 710 with three gas lasers, one LED, four detectors and the availability of a micro manipulator and an environmental chamber. We have four wide field microscopes. A Nikon Eclipse with an Acturus Laser Dissection module. A Nikon Eclipse with Cooled CCD and a second CMOS high resolution camera. A Leica DM6b which is controlled by MBF StereoInvestigator, has a Lumenera camera and can be used for Stereology of brain section. Finally an inverted Leica DMi8 microscope which uses a LeicaAFC camera which can be switched between high sensitive fluorescence/ colour imaging. 

Applications

Keywords - Biological: Cell Biology | Developmental Biology | Microbiology | Histology/Pathology | Neurobiology | Immunobiology | Drosophila | Bio-materials
Keywords -  Physical Sciences: Nanomaterials

Sample Preparation

Keywords - Biological: Serial sectioning | Cryosectioning | Immunofluorescence | Vibrating microtome | Enzyme histochemistry | Immunolabelling | Photoconversion | Cryostat sectioning | H&E staining | Live cell 

Data Analysis

Keywords - Software: ImageJ | Imaris | Fiji | Volocity 


Point of Contact

Faculty of Medicine and Dentistry University of Plymouth
Derriford Research Facility
16 Research Way
Plymouth, PL6 8BU , United Kingdom


Point of Contact

Torsten Bossing, 07929477815