Advanced Microscopy Facility, WT-MRC Stem Cell Institute

Expertise

Imaging Platforms

Keywords - Light Microscopy: Confocal microscopy | Widefield microscopy | Live cell imaging | Multi-photon microscopy | TCSPC-FLIM | Structured Illumination Microscopy

Leica Stellaris8 FLIM
Leica SP5 and Leica SP8
Nikon spinning disk
Zeiss LSM700 and LSM710 Intune/FCS/FLIM
Zeiss LSM 880 MP Insight DS+ Airyscan and LSM980 Airyscan2
Leica DMI6000
GE Incell Analyser
Nikon Biostation IM
Essen Incucyte Zoom
Zeiss AxioImager Apotome
Leica DMI4000
Olympus IX51 plate scanner

Hitachi 7800 TEM

Tescan Clara SEM, Oxford instruments EDS, Connectomx Katana serial sectioning in chamer microtome

Applications

Keywords - Biological: Cell Biology | Neurobiology | Developmental Biology | Immunobiology | Zebrafish | Drosophila | Bio-materials | Histology/pathology

Sample Preparation

Keywords - Biological: Serial sectioning | Immunolabelling | Immunofluorescence | Cryostat sectioning | Vibrating microtome | H&E staining | Enzyme histochemistry | Live Cell

Data Analysis

Keywords - Software: ImageJ | FIJI | Imaris | Volocity | iQ | AxioVision