Expertise
Imaging Platforms
Keywords - Light Microscopy: Superresolution microscopy | Laser microdissection | Live cell imaging | TCSPC-FLIM | Light-sheet microscopy (SPIM) | Confocal microscopy | Widefield microscopy | TIRF | Multi-photon microscopy | STORM | FCS/FCCS | Structured Illumination Microscopy
Keywords - Electron Microscopy: Scanning EM (FEG) | Focused Ion Beam SEM | Scanning Transmission EM
Volume EM | Correlative imaging.
Electron Microscopy: Zeiss CrossBeam 550 FIB SEM
Point-scanning confocal: Leica Stellaris 8, Nikon AX-R, Zeiss LSM 780
Spinning-disk confocal: Evident (Olympus) SoRa SpinSR
Multi-photon: Nikon AX-R
Super resolution: Nikon N-SIM/N-STORM
TIRF: Nikon Ti-2
Light-sheet: Miltenyi UltraScope Blaze, Leica Viventis Deep
Spatial Proteomics: Miltenyi MACSima
High content: Molecular Devices HT.ai
Long-term live: Sartorius Incucyte SX5
Slide Scanner: Zeiss AxioScan 7
Wide-field: Nikon Ti-2, Olympus cellSens
Laser Micro-dissection: Zeiss MicroBeam
Applications
Keywords – Biological: Cell Biology | Developmental Biology | C.elegans | Histology/Pathology | Neurobiology | Immunobiology | Drosophila
Primarily equipped for studying fixed and live biological specimens. Systems are configured to be as flexible as possible allowing a wide range of sample types.
Sample Preparation
Keywords - Biological: Resin embedding | Serial sectioning | Freeze substitution | Critical point drying | Correlative Light & EM | Immunofluorescence | Vibrating microtome | Ultrathin sectioning | Immunolabelling | High pressure freezing | Negative stain | Cryostat sectioning | H&E staining | Live cell
Keywords - Materials: Focused ion beam (Gallium)
Training on and access to tissue sectioning tools including cryostat, wax embedder, microtome, vibratome.
Electron Microscopy:
Training on and access to high pressure freezer, freeze substitution unit, critical point dryer, ultramicrotome.
Data Analysis
Keywords - Software: ImageJ | Imaris | Huygens | Fiji | Amira
Miltenyi MACS iQ, Leica LAS-X, Nikon Elements, CellProfiler, Napari, MIB
Keywords - Hardware: High spec workstations available for image processing and analysis
Full support and bespoke analysis service provided by Image Analyst
Shared Access
Full access for external users