Babraham Institute Imaging Facility (Cambridge)

The Babraham Institute's Imaging Facility provides supported access to cutting-edge microscopy systems and offers expert assistance for image processing and analysis. The Facility has light and electron microscopy resources, although the main focus is provision of fluorescence-based imaging. Expert advice and training is available on all platforms including confocal, multi-photon, high content and super resolution. External access (commercial and academic) is typically charged at an hourly rate.



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Expertise

Imaging Platforms

Keywords - Light Microscopy: Superresolution microscopy, Laser microdissection, Live cell imaging, Frequency-domain FLIM, Confocal microscopy, Widefield microscopy, TIRF, Multi-photon microscopy, FCS/FCCS, Structured Illumination Microscopy 

High content 

 
Keywords - Electron Microscopy: Scanning EM (FEG), Focused Ion Beam SEM, Scanning Transmission EM 

The BI Imaging Facility has a wide variety of imaging platforms. These are high-specification commercial systems that have been configured to accommodate a broad range of applications with both fixed and live specimens. Systems include point-scanning confocal (Leica Stellaris 8, Nikon A1-R, Zeiss 780), spinning disk confocal (Olympus SpinSR), multi-photon (Zeiss LSM-7 with Coherent Chameleon Vision+MPX), TIRF (Nikon), wide-field (Nikon and Olympus), High/Ultra-High Content (GE InCell 6000 and Miltenyi MACSima), Super Resolution (Nikon N-SIM/N-STORM) and laser micro-dissection (Zeiss MicroBeam). To complement fluorescence imaging capabilities, the Facility has a Zeiss CrossBeam 550 FIB SEM which offers various modalities for electron microscopy, including high resolution volumetric EM.

Applications

Keywords – Biological: Cell Biology, Developmental Biology, C.elegans, Histology/Pathology, Neurobiology, Immunobiology

The BI Imaging Facility has evolved to cater for the Institute's changing research needs, which currently focuses on understanding the molecular mechanisms underlying developmental and ageing processes. BI research is diverse, but grouped into the three main areas of signalling, epigenetics and immunology. The Imaging Facility accommodates a wide variety of applications within these areas and our equipment has been specified with flexibility in mind. 

Sample Preparation

Keywords - Biological: Resin embedding, Cryosectioning, Metal coating, Immunofluorescence, Vibrating microtome, Ultrathin sectioning, Immunolabelling, Negative stain, Photoconversion, Cryostat sectioning, Live cell  
Keywords - Materials: Focused ion beam (Gallium)

The Imaging Facility at Babraham has a limited sample preparation capability, although we can offer advice on what would be appropriate for use with our imaging systems.

Data Analysis

Keywords - Software: ImageJ, Imaris, Huygens, Fiji, Volocity

The BI Imaging Facility offers advice and services for image processing and analysis, including image analysis pipeline development. The Facility has a number of high specification PCs available for image processing, quantification and presentation. Training on a variety of different software packages is available on request. 

Shared Access

External users are welcome with access typically charged at an hourly rate (please contact the Facility for details). Institute users will get priority access to equipment.

Funding

The equipment in the BI Imaging Facility has been funded by the BBSRC. Operating the Facility and maintaining the equipment requires an annual budget which is funded through hourly charges. Please contact the Facility to find out what the current hourly rates. 



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Point of Contact

Babraham Institute Imaging Facility (Cambridge)
Imaging Facility, Babraham Institute
High Street
Babraham Research Campus
Cambridge, CB22 4AT, United Kingdom


Simon Walker, +44 (0)1223 496618

Staff List

Simon Walker (Imaging Facility Manager)
Hanneke Okkenhaug (Deputy Facility Manager)