Expertise
Imaging Platforms
Keywords - Light Microscopy: Cryo-fluorescence | Superresolution microscopy | Live Cell Imaging | Gated-STED | PALM | TCSPC-FLIM | Light-sheet microscopy (SPIM) | Confocal microscopy | Widefield microscopy | TIRF | Multi-photon microscopy | STORM | FCS/FCCS
OTHER: optical tweezers
Keywords – Electron Microscopy: Transmission EM | Electron tomography | Scanning EM (FEG) | Integrated Light and SEM | CRYO-ET | Scanning TRANSMISSION EM | SERIAL BLOCK FACE SEM | INTEGRATED LIGHT AND TEM | CRYO-TEM
OTHER: SCANNING EM (LAB6)
An extensive range of advanced fluorescence imaging systems (including confocal, widefield, multiphoton, super-resolution, TIRF, FLIM, FCS lightsheet and optical tweezers) are positioned alongside high specification electron microscopes (TEM, SEM, SBF-SEM) to provide exceptional opportunities for microscopy applications.
Applications
KEYWORDS - BIOLOGICAL: CALL BIOLOGY | DEVELOPMENTAL BIOLOGY | PLANT BIOLOGY | C.ELEGANS | MICROBIOLOGY | HISTOLOGY/PATHOLOGY | NEUROBIOLOGY | IMMUNOBIOLOGY | ZEBRAFISH | DROSOPHILA | BIO-MATERIALS
Keywords – Physical Sciences: Bioengineering and biomaterials | Nanomaterials
Sample Preparation
Keywords – Biological: Resin embedding | SERIAL SECTIONING | CRYOSECTIONING | Plunge Freezing | Freeze substitution | Critical Point Drying | Immunofluorescence | ENZYME HISTOCHEMISTRY | IMMUNOLABELLING | HIGH PRESSURE FREEZING | CEMOVIS | NEGATIVE STAIN | PHOTOCONVERSION | PLT | CRYOSTAT SECTIONING | Live Cell
Highly experienced support team can advise, train or provide service for most types of biological (and some material) sample preparation. Histology services are provided via our neighbouring histology facility - contact debbie.martin@bristol.ac.uk
Data Analysis
Keywords - Software: ImageJ | Huygens | FLIMfit | Fiji | Amira | Matlab | VOlocity | PYTHON | ARRIVIS VISION 4D
A data analysis suite provides access to commercial and open-source image processing and analysis tools. A dedicated image analyst - stephen.cross@bristol.ac.uk - provides advice, training and development of bespoke analysis tools.
Shared Acces
The Facility welcomes enquiries from potential external users. We are available for such use where capacity and compliance with the University of Bristol’s research governance and integrity policy allows. We operate on a Full Economic Costing recovery basis.
Funding
The Facility was formed in 2008 by the merger of the MRC Cell Imaging Facility (established 1996) with EM facilities in the Department of Physiology and the acquisition of new laboratory space and equipment (LM and EM systems) funded by the Wolfson Foundation and University of Bristol. The Facility has since grown to house 28 imaging systems funded by MRC, BBSRC, Wellcome Trust, EPSRC and University sources and expanded into additional rooms funded by the Wolfson Foundation.