Cancer Research UK Cambridge Institute

We offer a full range of educational and cytometric services including stem cell analysis, immunophenotyping, cell cycle analysis, translocation and co-localisation of cell activation markers, chromatin density, and apoptotic and necrotic analyses. We perform CL1 and CL2 cell sorting, including stem cell purification and rare cell sorting. We also offer expertise in experimental design and provide equipment training for all users.



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Expertise 

Platforms 

Keywords: Analytical Flow Cytometry | Cell sorting | Imaging Flow Cytometry | Mass Cytometry

Analyzers:
BD LSRFortessa SORP - 5L(UV/V/B/YG/R):18 fluorescence parameters, HTS 96-well loader attachment
BD FACSymphony A5 - 6L(UV/V/B/YG/R/IR):30 fluorescence parameters, HTS 96-well loader attachment.
MACSQuant VYB - 3L(V/B/YG):8 fluorescence parameters
CYTEK Aurora - 5L (UV/V/B/YG/R):64 fluorescence parameters
CYTEK Imagestream mkII - 4L(V/B/YG/R):11 fluorescence parameters

Cell Sorters:
BD FACSMelody - 3L(V/B/Y):8 fluorescence parameters
BD FACSymphony S6 - Contained in CL2 cabinet, 6L(UV/V/B/YG/R/IR):30 fluorescence parameters
BD FACSDiscover S8 - Real time Imaging capability, 5L(UV/V/B/YG/R):78 fluorescence parameters+3 Imaging parameters

Mass Cytometers:
Standard Biotools Helios Mass Cytometer
5x Standard Biotols Hyperion XTI Imaging Mass Cytometers

 

Applications 

Keywords: Phenotyping | Cell cycle | Mammalian cells | Bacteria | Fluorescent proteins | Functional studies

Stem cell analysis, immunophenotyping, cell cycle analysis, translocation and co-localisation of cell activation markers, chromatin density, and apoptotic and necrotic analyses.

 

Data Analysis

Keywords - Software: FlowJo | IDEAS

 

Shared Access

Facility is open to access for external users

Funding Overview

Facility operates in a cost recovery basis

 



Point of Contact

Cancer Research UK Cambridge Institute
University of Cambridge Li Ka Shing Centre
Robinson Way
Cambridge, CB2 0RE, United Kingdom


Facilities Contact

Richard Grenfell

Email: flowcytometry@cruk.cam.ac.uk
Website: Cancer Research UK Cambridge Institute

Staff List 

Richard Grenfell - Head of Flow Cytometry
Cantug Bar, PhD - Scientific Associate
Christopher Armstrong - Research Assistant